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Estimating Wetting Front Coordinates Under Surface Trickle Irrigation, Ahad Molavi, Aliashraf Sadraddini, Amir Hossein Nazemi, Ahmad Fakheri Fard Jan 2012

Estimating Wetting Front Coordinates Under Surface Trickle Irrigation, Ahad Molavi, Aliashraf Sadraddini, Amir Hossein Nazemi, Ahmad Fakheri Fard

Turkish Journal of Agriculture and Forestry

In this study, wetting front or wetted bulb coordinates in soil under surface trickle irrigation were measured for 1 loam soil and 2 sandy loam soils with 2 different emitter discharges of 2 and 4 L h^{-1} by using the trenching method. A model is presented for estimating wetted bulb coordinates with a function of emitter discharge, water application time, average variation in volumetric water content, and saturated hydraulic conductivity of soil. For calculating the distance of the maximum wet surface, relationships are presented based on saturated hydraulic conductivity and water application time. By comparison of measured values of wetting …


Purification And Characterization Of Endoxylanase Xln-2 From Aspergillus Niger B03, Georgi Dobrev, Boriana Zhekova Jan 2012

Purification And Characterization Of Endoxylanase Xln-2 From Aspergillus Niger B03, Georgi Dobrev, Boriana Zhekova

Turkish Journal of Biology

An extracellular multiple form of endoxylanase was isolated from the xylanolytic complex of Aspergillus niger B03. The enzyme was purified to a homogenous form using ultrafiltration, anion exchange chromatography, and gel filtration. It was a nonglycosylated protein with a molecular weight of 20,000 Da as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 21,000 Da as determined by gel filtration. The optimal pH for the enzyme action was 5.0 and the optimal temperature was 55 °C. Endoxylanase stability was significantly improved in the presence of glycerol and sorbitol. The enzyme activity was activated by Mn^{2+} and Co^{2+}, and it was …


Comparison Of Three Yarrowia Lipolytica Strains For Lipase Production: Nbrc 1658, Ifo 1195, And A Local Strain, Özgür Kebabci, Ni̇lüfer Ci̇hangi̇r Jan 2012

Comparison Of Three Yarrowia Lipolytica Strains For Lipase Production: Nbrc 1658, Ifo 1195, And A Local Strain, Özgür Kebabci, Ni̇lüfer Ci̇hangi̇r

Turkish Journal of Biology

Lipase production was examined with 3 strains of Yarrowia lipolytica: NBRC 1658, IFO 1195, and a local strain. All strains were obtained from Hacettepe University's Department of Food Engineering. The addition of nitrogen and carbon sources and the effects of initial pH and temperature parameters were studied. For all 3 strains, the growth temperature range was 20-40 °C, and maximum growth was determined at 30 °C. The initial pH range was 6-9 for growth and 3-6 for lipase production. Maximum growth results were detected as follows: Y. lipolytica NBRC 1658 at 30 °C and pH 8, Y. lipolytica IFO 1195 …


Prevalence Of The Ivs1(+1)G->A And 35delg Mutations In The Gjb2 Gene Of Turkish Patients With Nonsyndromic Hearing Loss, Tüzün Arik Biyikli Jan 2012

Prevalence Of The Ivs1(+1)G->A And 35delg Mutations In The Gjb2 Gene Of Turkish Patients With Nonsyndromic Hearing Loss, Tüzün Arik Biyikli

Turkish Journal of Biology

GJB2 encodes connexin 26, a gap junction protein that is assumed to be a component of the potassium recycling pathway in the inner ear. Loss or malfunction of these gap junctions, as might be reflected by mutations in GJB2, may disrupt potassium movement from the hair cells through the supporting cell network to the endolymph, leading to hearing impairment. One mutation, the deletion of 1 guanosine residue from a stretch of 6 between nucleotide positions 30 and 35 (35delG) at codon 10, is the most common deafness-causing allelic variant of GJB2 in sporadic patients and autosomal recessive families. Mutations in …


Phosphate-Solubilizing Microbes And Their Occurrence In The Rhizospheres Of Piper Betel In Karnataka, India, Padmavathi Tallapragada, Usha Seshachala Jan 2012

Phosphate-Solubilizing Microbes And Their Occurrence In The Rhizospheres Of Piper Betel In Karnataka, India, Padmavathi Tallapragada, Usha Seshachala

Turkish Journal of Biology

Low phosphate solubility is one of the most important factors limiting the plant growth in Indian soils. Many microorganisms can enhance phosphate solubility, but little is known about the magnitude of their phosphorus-solubilizing ability. The native populations of phosphate-solubilizing bacteria and fungi were studied in different rhizospheric soil samples obtained from betel vine plants (Piper betel L.) in order to compare the results. The present study focuses on the phosphate-solubilizing capacity of bacteria and fungi in rhizospheric soil samples obtained from betel vine plants, revealing the dominance of Aspergillus species (26.1 mm) as major phosphate solubilizers, along with Bacillus subtilis …


Anti-Inflammatory Effects Of Different Extracts From Three Salvia Species, Eli̇f Çadirci, Hali̇s Süleyman, Peri̇han Gürbüz, Ayşe Uz, Zühal Güvenalp, Lütfi̇ye Ömür Demi̇rezer Jan 2012

Anti-Inflammatory Effects Of Different Extracts From Three Salvia Species, Eli̇f Çadirci, Hali̇s Süleyman, Peri̇han Gürbüz, Ayşe Uz, Zühal Güvenalp, Lütfi̇ye Ömür Demi̇rezer

Turkish Journal of Biology

Salvia L. species have been used for the treatment of various inflammatory ailments in traditional medicine. In order to evaluate this ethnobotanical information, water, methanol, n-butanol, acetone, and chloroform extracts from 3 Salvia species (S. fruticosa, S. verticillata, and S. trichoclada) were screened for their anti-inflammatory activity using in vivo experimental models in rats. For this purpose a carrageenan-induced inflammatory paw edema model was used. All extracts demonstrated anti-inflammatory activities; however, n-butanol extract of Salvia fruticosa (syn. S. triloba), which is known as Turkish sage, was found to be the most active. It can be expected that the active flavonoids, …


Protective Effects Of \Beta-Carotene And Silymarin On Human Lymphocytes, Erkan Yurtcu, Ezgi̇ Kasapoğlu, Feri̇de İffet Şahi̇n Jan 2012

Protective Effects Of \Beta-Carotene And Silymarin On Human Lymphocytes, Erkan Yurtcu, Ezgi̇ Kasapoğlu, Feri̇de İffet Şahi̇n

Turkish Journal of Biology

Beta-carotene and silymarin have antioxidant properties against oxidative damage and are used as dietary supplements. The aim of this study was to assess the protective effects of \beta-carotene and silymarin on healthy human lymphocytes exposed to L-arginine-induced oxidative damage. Study samples were lymphocyte cultures set up from venous blood obtained from 6 healthy individuals (3 males and 3 females). Oxidative DNA damage was induced by L-arginine. \beta-Carotene and silymarin were added to the cultures as antioxidants to observe their protective effects, if any. Alkaline single-cell gel electrophoresis (SCGE) was used to evaluate DNA damage. While L-arginine generated genotoxic damage in …


Folk Medicines In The Villages Of Ilıca District (Erzurum, Turkey), Ufuk Özgen, Yusuf Kaya, Peter Houghton Jan 2012

Folk Medicines In The Villages Of Ilıca District (Erzurum, Turkey), Ufuk Özgen, Yusuf Kaya, Peter Houghton

Turkish Journal of Biology

The villages of Ilıca District in Erzurum Province, Turkey, were surveyed in terms of plants and animals used traditionally for medicinal purposes. Plants were compiled according to their families, botanical names, local names, usages, and applications. A total of 70 plant species belonging to 29 families are used by village people to treat a wide range of complaints, such as hemorrhoids, urinary system infections, stomach disorders, respiratory diseases, skin diseases, rheumatism, and jaundice. In addition, 3 animal species are used for the treatment of some diseases. The collected data were compared with those formerly recorded.


Cytotoxic And Antibacterial Activities Of Leaf Extracts Of Astragalus Gombiformis Pomel (Fabaceae) Growing Wild In Tunisia, Hassen Teyeb, Nahla Zanina, Mohamed Neffati, Wahiba Douki, Mohamed Fadhel Najjar Jan 2012

Cytotoxic And Antibacterial Activities Of Leaf Extracts Of Astragalus Gombiformis Pomel (Fabaceae) Growing Wild In Tunisia, Hassen Teyeb, Nahla Zanina, Mohamed Neffati, Wahiba Douki, Mohamed Fadhel Najjar

Turkish Journal of Biology

Many species of the genus Astragalus have been long used in folk medicine because of their biological properties. The aim of this study was to investigate the antibacterial and cytotoxic activities of extracts from the leaves of wild Astragalus gombiformis Pomel. Antibacterial activity was assayed against several common human pathogenic bacterial strains using the paper disk-agar diffusion method. Cytotoxicity was measured against human lung carcinoma cells using the colorimetric MTT assay. The strongest cytotoxic activity against the human A549 lung epithelial carcinoma cell line was detected for dichloromethane extracts at IC_{50} = 85 ± 21.7 µg/mL. The best incubation time …


Antimicrobial And Antioxidant Properties Of Artemisia L. Species From Western Anatolia, Şüra Baykan Erel, Gottfried Reznicek, Serdar Gökhan Şenol, Nefi̇se Ülkü Karabay Yavaşoğlu, Si̇bel Konyalioğlu, Ahmet Ulvi̇ Zeybek Jan 2012

Antimicrobial And Antioxidant Properties Of Artemisia L. Species From Western Anatolia, Şüra Baykan Erel, Gottfried Reznicek, Serdar Gökhan Şenol, Nefi̇se Ülkü Karabay Yavaşoğlu, Si̇bel Konyalioğlu, Ahmet Ulvi̇ Zeybek

Turkish Journal of Biology

Artemisia L. taxa (A. absinthium L., A. arborescens L., A. campestris L., A. scoparia Waldst. & Kit., A. santonicum L., and A. vulgaris L.) naturally distributed through western and southwestern Turkey were chosen as experimental materials in this study. Essential oils of the aerial parts of these Artemisia species were isolated by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The major components were identified as sabinene in A. absinthium (17.56%), camphor in A. arborescens (33.39%), 1,2-dehydro acenaphthylene in A. campestris and A. scoparia (20.71% and 11.80%, respectively), and \alpha-thujone in A. vulgaris and A. santonicum (56.13% and 39.46%, respectively). Essential …


Antimicrobial Activity Of Poplar Propolis On Mutans Streptococci And Caries Development In Rats, Soley Arslan, Si̇bel Si̇li̇ci̇, Duygu Perçi̇n, Ayşe Nedret Koç, Özgür Er Jan 2012

Antimicrobial Activity Of Poplar Propolis On Mutans Streptococci And Caries Development In Rats, Soley Arslan, Si̇bel Si̇li̇ci̇, Duygu Perçi̇n, Ayşe Nedret Koç, Özgür Er

Turkish Journal of Biology

The influence of the different extracts of propolis and chlorhexidine on mutans streptococci and the development of dental caries in rats was examined in this study. Specifically, the antimicrobial effects of propolis extracts (including ethanol, methanol, chloroform, hexane, propylene glycol, and ethyl acetate) on Streptococcus mutans UA159 and Streptococcus sobrinus 6715 were studied. Minimal inhibitory concentration and minimal bactericidal concentration were determined using the National Committee for Clinical Laboratory Standards broth macrodilution method. In the animal study, the rats were infected with S. sobrinus and fed a cariogenic diet. They were treated topically twice a day with both ethanol extract …


Determination Of Mutagenic Potencies Of Aqueous Extracts Of Thermopsis Turcica By Ames Test, Recep Li̇man, Yasi̇n Eren, Di̇lek Akyil, Muhsi̇n Konuk Jan 2012

Determination Of Mutagenic Potencies Of Aqueous Extracts Of Thermopsis Turcica By Ames Test, Recep Li̇man, Yasi̇n Eren, Di̇lek Akyil, Muhsi̇n Konuk

Turkish Journal of Biology

Thermopsis turcica is an endemic species of Turkey popularly known as Eber Sarısı or Piyam; it is a member of the Thermopsis genus, which contains plants considered to be medicinal throughout the world. In this study, the mutagenic effect of aqueous extracts obtained from the leaves, root, stem, and flowers of T. turcica were evaluated by the Ames test, using Salmonella typhimurium TA97, TA98, TA100, and TA102 strains with and without an S9 activation system. The results obtained were analyzed statistically using SPSS for Windows, and the Mann-Whitney test was performed for comparisons. The results showed that all of the …


Changes In Seed Reserve Composition During Germination And Initial Seedling Development Of Safflower (Carthamus Tinctorius L.), Muhammet Tonguç, Ruzi̇ye Elkoyunu, Sabri̇ Erbaş, Yaşar Karakurt Jan 2012

Changes In Seed Reserve Composition During Germination And Initial Seedling Development Of Safflower (Carthamus Tinctorius L.), Muhammet Tonguç, Ruzi̇ye Elkoyunu, Sabri̇ Erbaş, Yaşar Karakurt

Turkish Journal of Biology

The present study was carried out to determine the changes in some reserve molecules of safflower seeds during germination and early seedling growth. The seeds of 2 safflower cultivars were used as plant material (Dinçer 5-118 and Montola 2000). Seeds were germinated for 72 h and observations were taken every 12 h throughout the study. Changes in total oil content (%), total protein content (%), total soluble sugar contents (TSS, mg g^{-1}), and reducing sugar contents (RS, mg g^{-1}) were determined for each cultivar during germination and seedling growth. The oil content of the cultivars decreased from 54.7% and 57.4% …


The Growth And Reproduction Of White Bream (Blicca Bjoerkna L. 1758) In An Oligo-Mesotrophic Lake In Northwest Anatolia (Sapanca, Turkey), Hacer Canan Okgerman, Mahmut Elp, Si̇bel Atasağun Jan 2012

The Growth And Reproduction Of White Bream (Blicca Bjoerkna L. 1758) In An Oligo-Mesotrophic Lake In Northwest Anatolia (Sapanca, Turkey), Hacer Canan Okgerman, Mahmut Elp, Si̇bel Atasağun

Turkish Journal of Biology

Growth parameters and the reproduction period of white bream (Blicca bjoerkna L. 1758) sampled from Sapanca Lake were determined monthly between November 2000 and October 2001. Three hundred and fifty white bream specimens caught ranged in age from 1^+ to 9^+ years, and the majority of the samples consisted of individuals of the 5^+-year-old age group. The von Bertalanffy equation was determined as Lt = 22.169 (1 - exp {-0.215[t + 0.986]}), Ø': 2.02 for males and Lt = 31.917 (1 - exp {-0.122[t + 1.087]}), Ø': 2.10 for females. The length-weight relationship was estimated as W = 0.0046 TL …


The Effects Of Seasonal Variation On The Fatty Acid Composition Of Total Lipid, Phospholipid, And Triacylglycerol In The Dorsal Muscle Of Capoeta Trutta Found In The Tigris River (Turkey), Eli̇f İpek Satar, Ersi̇n Uysal, Erhan Ünlü, Mehmet Başhan, Ali̇ Satar Jan 2012

The Effects Of Seasonal Variation On The Fatty Acid Composition Of Total Lipid, Phospholipid, And Triacylglycerol In The Dorsal Muscle Of Capoeta Trutta Found In The Tigris River (Turkey), Eli̇f İpek Satar, Ersi̇n Uysal, Erhan Ünlü, Mehmet Başhan, Ali̇ Satar

Turkish Journal of Biology

The effects of seasonal variation on the fatty acid composition of total lipid, triacylglycerol, and phospholipid in the dorsal muscle of Capoeta trutta were assessed by gas chromatographic method. A total of 18 different fatty acids were determined in the composition of C. trutta. The variations in fatty acid composition of total lipid, triacylglycerol, and phospholipid were recorded throughout the seasons. The total polyunsaturated fatty acids (PUFA) and monounsaturated fatty acids (MUFA) were the most important groups of total lipid. Total saturated fatty acid (SFA) and total MUFA were seen to increase from spring to summer while total PUFA decreased …


Effects Of Compatibilizer And Nanolayered Silicate On Physical And Mechanical Properties Of Pp/Bagasse Composites, Behzad Kord Jan 2012

Effects Of Compatibilizer And Nanolayered Silicate On Physical And Mechanical Properties Of Pp/Bagasse Composites, Behzad Kord

Turkish Journal of Agriculture and Forestry

Bagasse, an agricultural waste, was investigated for wood plastic nanocomposite production. The incorporation of nanolayered silicate has been considered in recent years for improving the properties of thermoplastic composite. In this study a composite of polypropylene (PP) and bagasse fiber (BF), with different concentrations of nanoclay and maleic anhydride (MA) as compatibilizer, were fabricated by injection molding. Then physical and mechanical properties of the composites were tested according to ASTM standards. The results indicated that tensile modulus and flexural strength increased with an increase in clay loading; however, impact strength and water absorption decreased with an increase in nanolayered silicates. …


Larval Parasitoids And Larval Diseases Of Malacosoma Neustria L. (Lepidoptera: Lasiocampidae) Detected In Erzurum Province, Turkey, Hi̇kmet Özbek, Sali̇ha Çoruh Jan 2012

Larval Parasitoids And Larval Diseases Of Malacosoma Neustria L. (Lepidoptera: Lasiocampidae) Detected In Erzurum Province, Turkey, Hi̇kmet Özbek, Sali̇ha Çoruh

Turkish Journal of Zoology

The larval parasitoids and larval diseases of Malacosoma neustria (L.) (Lepidoptera: Lasiocampidae) in various host plants were investigated in Erzurum Province, Turkey, during 2004 and 2005. The larval parasitoids were reared in a laboratory, and a total of 29 parasitoid species were established in association with the larvae of M. neustria in the families Tachinidae (Diptera), Braconidae, Ichneumonidae, and Chalcidoidea (Hymenoptera). Of the 6 tachinid parasitoids, Pales pavida (Meigen) was the most prevalent species and accounted for 25% of the tachinid records. Among the tachinids, there were 2 new records for the Turkish fauna: Exorista amoena Mesnil and Palesisa nudioculata …


Additional Notes On The Spider Fauna Of Turkey (Araneae), Kadi̇r Buğaç Kunt, Rahşen Saltanat Kaya, Recep Sulhi̇ Özkütük, Tarik Danişman, Ersen Aydin Yağmur, Mert Elveri̇ci̇ Jan 2012

Additional Notes On The Spider Fauna Of Turkey (Araneae), Kadi̇r Buğaç Kunt, Rahşen Saltanat Kaya, Recep Sulhi̇ Özkütük, Tarik Danişman, Ersen Aydin Yağmur, Mert Elveri̇ci̇

Turkish Journal of Zoology

This paper presents the results of recent field surveys and provides new data on the spider fauna of Turkey. The following 9 taxa are new records for Turkey: Larinia chloris (Audouin, 1826); Macrothele sp.; Arctosa tbilisiensis Mcheidze, 1946; Pisaura consocia (O. P.-Cambridge, 1872); Prodidomus redikorzevi Spassky, 1940; Macaroeris flavicomis (Simon, 1884); Scytodes velutina Heineken & Lowe, 1832; Achaeridion conigerum (Simon, 1914); and Phoroncidia paradoxa (Lucas, 1846). New locality data are provided for the following previously but rarely recorded species: Spermophora senoculata (Dugès, 1836); Ariadna insidiatrix Audouin, 1826; and Segestria senoculata (Linnaeus, 1758). Taxonomic, zoogeographical, and ecological data are given for …


Agrobacterium-Mediated Transformation Of Grapefruit With The Wild-Type And Mutant Rna-Dependent Rna Polymerase Genes Of Citrus Tristeza Virus, Bayram Çevi̇k, Richard F. Lee, Charles L. Niblett Jan 2012

Agrobacterium-Mediated Transformation Of Grapefruit With The Wild-Type And Mutant Rna-Dependent Rna Polymerase Genes Of Citrus Tristeza Virus, Bayram Çevi̇k, Richard F. Lee, Charles L. Niblett

Turkish Journal of Agriculture and Forestry

Citrus paradisi Macfad. ‘Duncan’ was transformed with constructs coding for the wild-type and mutant RNA-dependent RNA polymerase (RdRp) of Citrus tristeza virus (CTV) for exploring replicase-mediated pathogen-derived resistance (RM-PDR). The RdRp gene was amplified from a CTV genome and used to generate the wild-type and 2 mutant RdRp constructs for plant transformation. One mutant had the key amino acids GDD changed to AAA (RdRp-mGDD), and the second mutant had a deletion encompassing the GDD domain (RdRp-\DeltaGDD). Etiolated epicotyl segments of Duncan grapefruit (Citrus paradisi Macfad. 'Duncan') were transformed with each of these constructs using the Agrobacterium-mediated transformation method. From 4540 …


Effect Of Wetted Soil Area On Trunk Growth, Yield, And Fruit Quality Of Drip-Irrigated Sour Cherry Trees, Osman Yildirim, Hati̇ce Dumanoğlu, Nurdan Güneş, Murat Yildirim, Ahmet Aygün, Beki̇r Şan Jan 2012

Effect Of Wetted Soil Area On Trunk Growth, Yield, And Fruit Quality Of Drip-Irrigated Sour Cherry Trees, Osman Yildirim, Hati̇ce Dumanoğlu, Nurdan Güneş, Murat Yildirim, Ahmet Aygün, Beki̇r Şan

Turkish Journal of Agriculture and Forestry

The effect of wetted soil area on trunk growth, yield, and fruit quality in drip-irrigated Kütahya sour cherry (Prunus cerasus L.) trees grafted on mahaleb (Prunus mahaleb L.) seedlings was investigated over 3 seasons in a semiarid zone of Central Anatolia, Turkey. Percentages of wetted soil area ranging from 8.8% to 35.6% were obtained by applying 7 treatments consisting of 1 or 2 lateral drip lines per tree row and various dripper numbers per tree. Adequate irrigation was applied to all treatments based on daily evapotranspiration values estimated by the FAO-modified Penman-Monteith equation. The highest yield was generally obtained at …


The Expression Of Gst Isoenzymes In Acinar Adenocarcinoma, Intraepithelial Neoplasia, And Benign Prostate Tissue: Correlation Of Clinical Parameters With Gst Isoenzymes, Gülçi̇n Şi̇mşek, Serpi̇l Oğuztüzün, Servet Güreşçi̇, Murat Kiliç, Ömer Faruk Bozkurt, Ali̇ Ünsal Jan 2012

The Expression Of Gst Isoenzymes In Acinar Adenocarcinoma, Intraepithelial Neoplasia, And Benign Prostate Tissue: Correlation Of Clinical Parameters With Gst Isoenzymes, Gülçi̇n Şi̇mşek, Serpi̇l Oğuztüzün, Servet Güreşçi̇, Murat Kiliç, Ömer Faruk Bozkurt, Ali̇ Ünsal

Turkish Journal of Biology

This study investigated the immunohistochemical staining characteristics of glutathione-S-transferase (GST) alpha, pi, mu, and theta in prostatic acinar adenocarcinoma (PCA), prostatic intraepithelial neoplasia (PIN), and benign prostatic tissues from 19 patients. Relationships between GST isoenzyme expression in benign, PIN, and PCA tissue were examined by the Wilcoxon signed-rank test and clinicopathological data were examined by the Spearman correlation rank test. When the benign, PIN, and PCA tissues from these cases were compared according to their staining intensity, GST alpha, pi, mu, and theta expressions in tumor cells were significantly lower than in benign epithelial cells (P < 0.05). The GST alpha class displayed the lowest level of expression in PIN and PCA. Expression of GST pi was lower in PCA tissue than in PIN and benign epithelial tissue (P < 0.05). We hypothesize that carcinogenesis in the prostate results from impaired cellular handling of mutagenic agents owing to reduction or loss of expression of multiple GST isoenzymes and other detoxifying and antimutagenesis agents. This study confirms the down-regulation of GST isoenzymes in PCA of the prostate and shows that the loss of GST isoenzyme expression is a phenotype associated with malignant transformation. There was no statistical relationship between GST isoenzyme expression and the clinicopathological data (age, Gleason score, and total serum prostate-specific antigen levels) (P > 0.05).


Induction Of Salt-Tolerant Potato (Solanum Tuberosum L.) Mutants With Gamma Irradiation And Characterization Of Genetic Variations Via Rapd-Pcr Analysis, Orkun Yaycili, Sema Ali̇kamanoğlu Jan 2012

Induction Of Salt-Tolerant Potato (Solanum Tuberosum L.) Mutants With Gamma Irradiation And Characterization Of Genetic Variations Via Rapd-Pcr Analysis, Orkun Yaycili, Sema Ali̇kamanoğlu

Turkish Journal of Biology

Salt-tolerant mutants of potato (Solanum tuberosum L. 'Marfona') were obtained via gamma irradiation. Node explants of the Marfona potato plant were treated with various dosages of gamma irradiation, and the M_1V_2 and M_1V_3 clonal generations were developed. Selection of salt-tolerant mutants was accomplished by in vitro selection media containing 50, 100, and 125 mM NaCl. Molecular-level differences between the control and mutant plants were elucidated using RAPD-PCR method, and the polymorphism rate according to the selected primers was calculated as 89.66%. Genetic distances between the controls and mutants were also calculated, and related dendrograms were produced. On average the mutants …


Dats Suppresses Invasion Of Oral Squamous Cell Carcinoma Cell Lines By Reducing Matrix Metalloproteinase-9 Via Pi3k/Akt, Jian Bin Yang, Dong Yi Wei, Zhi Yan Wu, Su Hua Xu Jan 2012

Dats Suppresses Invasion Of Oral Squamous Cell Carcinoma Cell Lines By Reducing Matrix Metalloproteinase-9 Via Pi3k/Akt, Jian Bin Yang, Dong Yi Wei, Zhi Yan Wu, Su Hua Xu

Turkish Journal of Biology

Oral squamous cell carcinomas (OSCCs) are the most common type of oral cancers, and high morbidity and mortality are largely attributable to late-stage diagnosis. Despite significant advances in therapeutic strategies, the 5-year survival rate still remains low. Diallyl trisulfide (DATS), an oil-soluble organosulfur compound of garlic, was recently reported for its anticancer effect on several types of cancer cells in vitro; however, these anticancer effects are still unknown in human OSCC cells. In the present study, the effects of DATS on OSCC cell invasion in vitro were investigated. Human OSCC cell lines (HSC-2 and Ca9-22 cells) were treated with DATS …


Morphological And 18s Rrna Analysis Of Coccoid Green Algae Isolated From Lakes Of Kızılırmak Delta, Eli̇f Neyran Soylu, Ari̇f Gönülol Jan 2012

Morphological And 18s Rrna Analysis Of Coccoid Green Algae Isolated From Lakes Of Kızılırmak Delta, Eli̇f Neyran Soylu, Ari̇f Gönülol

Turkish Journal of Biology

Molecular techniques are used today in many areas and have increased the confidence level of studies. The aim of the study was to compare the classical morphological characters of coccoid green algae under field and culture conditions by using modern molecular analysis. To achieve this aim, we first isolated the coccoid green algae from Liman and Cernek lakes. The morphological variations of green algae were observed under culture conditions and the phylogenetic relationships of these strains were defined according to the 18S rRNA gene sequences. According to sequence analysis, 2 of the isolated species had high similarity to Scenedesmus subspicatus …


Application Of Data Mining On Evaluation Of Energy Dissipation Over Low Gabion-Stepped Weir, Farzin Salmasi, Mohammad Taghi Sattari, Mahesh Pal Jan 2012

Application Of Data Mining On Evaluation Of Energy Dissipation Over Low Gabion-Stepped Weir, Farzin Salmasi, Mohammad Taghi Sattari, Mahesh Pal

Turkish Journal of Agriculture and Forestry

Results of a study designed to examine the behavior of gabion-stepped weirs for energy dissipation are presented in this paper. Laboratory tests were conducted with 8 physical models consisting of 3 different porosities (38%, 40%, and 42%) and 2 slopes (1:1 and 1:2). An iron plate was also placed on each horizontal and vertical step to study and classify the effect of step porosity on the energy dissipation rate. A decision tree technique was used to derive if-then rules in order to classify the energy dissipation through the weir models. Results from this study suggest that a decision tree model …


Combined Treatment Of Modified Atmosphere Packaging And 1-Methylcyclopropene Improves Postharvest Quality Of Japanese Plums, Mustafa Erkan, Hakan Eski̇ Jan 2012

Combined Treatment Of Modified Atmosphere Packaging And 1-Methylcyclopropene Improves Postharvest Quality Of Japanese Plums, Mustafa Erkan, Hakan Eski̇

Turkish Journal of Agriculture and Forestry

Two Japanese-type plum (Prunus salicina Lindell) cultivars, 'Autumn Giant' and 'Black Beauty', were harvested at commercial maturity stage and either packed in nonperforated modified atmosphere packaging (MAP) or treated with a 500 ppb dose of 1-methylcyclopropene (1-MCP) alone or given a combination of 1-MCP and MAP. After these treatments, the plum samples were stored at 0 °C for 60 days. Treatment with either 1-MCP or modified atmosphere packaging (MAP) alone or with the 1-MCP+MAP combination reduced fruit softening, ethylene production, respiration rate, weight loss, and flesh browning, and resulted in higher flesh firmness, titratable acidity, and total soluble solids as …


Phylogenetic Characterization, Antimicrobial Susceptibilities, And Mechanisms Of Resistance In Bacteria Isolates From A Poultry Waste-Polluted River, Southwestern Nigeria, Olawale Olufemi Adelowo, Obasola Ezekiel Fagade Jan 2012

Phylogenetic Characterization, Antimicrobial Susceptibilities, And Mechanisms Of Resistance In Bacteria Isolates From A Poultry Waste-Polluted River, Southwestern Nigeria, Olawale Olufemi Adelowo, Obasola Ezekiel Fagade

Turkish Journal of Biology

Bacteria resistance to antibiotics has become a worldwide problem impacted partly by antibiotics use in livestock production. The present study investigated the pattern and mechanisms of resistance in 21 bacteria species isolated from a river used as a waste-dump by a poultry farm in Ogbomoso, southwestern Nigeria. Bacteria were isolated on nutrient agar supplemented with 50 µg/mL of tetracycline, their identities were determined by PCR amplification, and sequencing of their 16s rRNA genes and their susceptibility to 6 antimicrobials determined by broth dilution. The molecular basis of resistance among the isolates was also investigated by the PCR amplification of class …


Root Length, Root Mass, And Distribution Of Dry Matter In Different Parts Of Thompson Seedless Grapevine Grafted On Different Rootstocks In Heavy Soil Of Maharashtra, Ramhari G. Somkuwar, Pravin B. Taware, Devanand D. Bondage, Supriya Navale Jan 2012

Root Length, Root Mass, And Distribution Of Dry Matter In Different Parts Of Thompson Seedless Grapevine Grafted On Different Rootstocks In Heavy Soil Of Maharashtra, Ramhari G. Somkuwar, Pravin B. Taware, Devanand D. Bondage, Supriya Navale

Turkish Journal of Agriculture and Forestry

The root distribution pattern of 3 grape rootstocks, Dog Ridge, Salt Creek, and St. George, grafted with Thompson Seedless grapevine was studied at the National Research Centre for Grapes in Pune, India. The soil is heavy black cotton soil (vertisol) with a pH of >8.0. Roots of different thicknesses (10 mm) were examined at horizontal distances of 0-30 cm, 31-60 cm, 61-90 cm, 91-120 cm, and 121-150 cm away from the trunk and also to depths of 0-30 cm, 31-60 cm, and 61-90 cm from the surface. Among the different categories of roots, Dog Ridge put forth the maximum root …


Optimizing Water And Nitrogen Use For Maize Production Under Semiarid Conditions, Hafiz Mohkum Hammad, Ashfaq Ahmad, Farhat Abbas, Wajid Farhad Jan 2012

Optimizing Water And Nitrogen Use For Maize Production Under Semiarid Conditions, Hafiz Mohkum Hammad, Ashfaq Ahmad, Farhat Abbas, Wajid Farhad

Turkish Journal of Agriculture and Forestry

Water and nitrogen are among the most important crop inputs for optimum production of maize (Zea mays L.). A comprehensive experiment was conducted during 2009 and 2010 under the semiarid conditions of Pakistan to evaluate the effects of water and N applications on the growth and yield of irrigated maize. The objective was to formulate water and N best management practices (BMPs) for the above conditions. Three irrigation regimes (525, 450, and 375 mm ha^{-1}) with 5 N rates (0, 75, 150, 225, and 300 kg ha^{-1}) were tested using a split plot with a randomized complete block design. The …


Use Of Discriminant Analysis For Selection Of Hybrid Maize Parent Lines, Ahmet Öz Jan 2012

Use Of Discriminant Analysis For Selection Of Hybrid Maize Parent Lines, Ahmet Öz

Turkish Journal of Agriculture and Forestry

This study was conducted to investigate the opportunity to utilize discriminant analysis in the selection of parent lines in hybrid maize breeding. For this purpose, top crosses were obtained by top-crossing 31 inbred maize lines with the FRMo.17 line in 2003. These hybrids were subjected to preyield trials in 2004. The same lines were subjected to observation tests in 2003 and 2004, and yield and yield components were determined. The obtained data were analyzed using the discriminant analysis method. The distances between the lines were determined according to D2 and canonical values. The TK-108, TK-110, TK-13, TK-347, TK-175, TK-157, and …