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Advancing Biological Applications Through Microfluidic-Based Tool Development, K.M. Taufiqur Rahman
Advancing Biological Applications Through Microfluidic-Based Tool Development, K.M. Taufiqur Rahman
Electronic Theses and Dissertations
This research undertook an interdisciplinary approach, integrating bioengineering, microbiology, molecular biology, and systems biology to investigate bacterial dynamics behavior. Specifically, it delved into the development of microfluidic devices for biological applications such as bacterial cell counts, real-time observation of plant roots (here, specialized lectin-coated microbeads are used that mimic root characteristics), and soil microbe interactions. Furthermore, Next-Generation Sequencing and systems biology methodologies were employed to explore the intricate, multifaceted survival mechanisms of Escherichia coli persister population. Studying and quantifying persisters or testing for the existence of VBNC (viable but nonculturable) is challenging. These experiments require precise counts. It has been …
Nutrient Intake, Performance, And Body Composition In Preseason Wrestlers, Gregory Coapstick
Nutrient Intake, Performance, And Body Composition In Preseason Wrestlers, Gregory Coapstick
Electronic Theses and Dissertations
BACKGROUND: College athletes, especially in weight class sports, often experience energy deficits. Weight class sports such as wrestling are at greatest risk for deficiencies, and little is known about the relationships between body composition, nutrient intake, and performance in these athletes.
OBJECTIVES: The purposes of this study were to quantify macronutrient and micronutrient intake of pre-season male collegiate wrestlers and compare to recommendations, and examine relationships among nutritional intakes, body composition, and performance measurements of strength, anaerobic, and aerobic capacity.
METHODS: Male Division I wrestlers (n=11, mean ± SD age: 21.3±1.7 years, wrestling experience: 14.9±2.5 years) were recruited during preseason. …
Nitrogen Application Timing And Cereal Rye (Secale Cereale L.) Cover Crop Influence Greenhouse Gas Emissions, Graig Reicks
Nitrogen Application Timing And Cereal Rye (Secale Cereale L.) Cover Crop Influence Greenhouse Gas Emissions, Graig Reicks
Electronic Theses and Dissertations
Dryland contributions to greenhouse gas (GHG) emissions are increased by N fertilizer applications and high soil water contents. Fertilizer timing and spring growth of cover crops prior to cash crop planting were investigated in separate studies to examine impacts on overall GHG emissions using a near continuous measurement system. There was a significant interaction between N fertilizer rate (0 vs. 224 kg N ha-1 surface-applied as urea) and application date (p= 0.01) for CO2 emissions. This interaction occurred because N fertilizer increased CO2 emissions by 35% for the 21 d interval following early spring application. When application was delayed until …
Physicochemical And Functional Properties Of Different Varieties Of Spring And Winter Peas Of South Dakota, Prakriti Dhakal
Physicochemical And Functional Properties Of Different Varieties Of Spring And Winter Peas Of South Dakota, Prakriti Dhakal
Electronic Theses and Dissertations
Peas are a good source of nutrients for both humans and animals, and they are the world's second most significant and sustainable legume. There have been studies on different kinds of pea grown in other parts of the world, but there is no information on the compositional and functional characteristics of pea varieties grown specifically in South Dakota. The objective of the study was to evaluate the proximate composition and functional properties of various pea varieties and determine if the pea variety impacted cookie properties. The proximate composition (moisture, protein, starch, and amylose), functional capacities (water holding, oil holding capacity, …
Evaluation Of Soybean Meal Versus Distillers Co-Products As A Protein Supplement For Finishing Beef Cattle: Effects On Growth Performance, Carcass Characteristics, And Efficiency Of Dietary Net Energy Utilization, Cassidy R. Ross
Electronic Theses and Dissertations
Two randomized complete block design experiments were conducted to: 1) examine the effects of soybean meal with or without additional soybean hulls in replacement of modified corn distillers grains plus (MDGS) solubles on growth performance efficiency of dietary net energy utilization and carcass traits responses in finishing beef steers, 2) determine if partial or complete replacement of dried distillers grains plus solubles (DDGS) with solvent-extracted soybean meal (SBM) in diets based upon high-moisture ensiled corn influences growth performance, efficiency of dietary net energy utilization, sera urea-N (SUN) concentrations, or carcass traits in finishing beef steers. Experiment 1 was a 118-d …
Species Distribution Modeling Of Aquilegia Brevistyla (Ranunculaceae): A Critically Imperiled Black Hills Disjunct Species, Eric Daniel Puetz
Species Distribution Modeling Of Aquilegia Brevistyla (Ranunculaceae): A Critically Imperiled Black Hills Disjunct Species, Eric Daniel Puetz
Electronic Theses and Dissertations
Unchecked human activity is contributing to rising levels of greenhouse gas emissions, changes in land use, altered disturbance/fire regimes, spread of invasive species, and loss of biological diversity and related breakdown of ecosystem services. Additionally, climatic shifts may lead to phenological mismatches between species and their environments if these changes outpace a species’ ability to adapt or migrate to a more suitable habitat. Isolated mountain populations are particularly threatened by unpredictable climatic conditions, as they may have limited migration corridors and often lower levels of genetic diversity to move or adapt, respectively. As these negative feedbacks compound on the landscape, …
Integrating Early Season Clover Cover Crops As A Living Mulch In Broccoli And Transitional Organic Winter Squash Production In Eastern South Dakota, Alexis Rose Barnes
Integrating Early Season Clover Cover Crops As A Living Mulch In Broccoli And Transitional Organic Winter Squash Production In Eastern South Dakota, Alexis Rose Barnes
Electronic Theses and Dissertations
Managing weeds, improving soil health, and reducing the use of plastic mulch continue to be priorities for South Dakota vegetable farmers. Farmers have expressed an interest in integrating cover crops into their farm systems. Clover cover crops used as a living mulch within and along cash crop rows may aid in weed suppression, nitrogen fixation, and prevent soil erosion. However, prior research has shown challenges of incorporating living mulch due to yield decreases. Research conducted in 2022 and 2023 in eastern South Dakota investigated the effects of four clover and four in-row soil management treatments on broccoli and organic winter …
The Hutton Project: Long-Term Agricultural Impacts On Soil Loss And Carbon Dynamics In Eastern South Dakota, Eli Halverson
The Hutton Project: Long-Term Agricultural Impacts On Soil Loss And Carbon Dynamics In Eastern South Dakota, Eli Halverson
Electronic Theses and Dissertations
Long-term and intensified agricultural land management has resulted in increased rates of soil erosion and has altered much of the carbon cycle at regional and global scales. Anthropogenic degradation of soil resources is a barrier to sustainable production, soil functioning, and ecosystem services. It is difficult to quantify the scope of pedogenic changes due to the lack of legacy data and short temporal scales. This study utilized decades to century-old soil information to quantify historical soil erosion losses and changes in soil carbon pools of eastern South Dakota soils. The results show that soils in the region have been significantly …
The Spatial Ecology Of Plains Spotted Skunks In South Dakota: Insights From Species Distribution, Resource Selection, And Co-Occurrence Models, Kara M. White
Electronic Theses and Dissertations
The plains spotted skunk (Spilogale interrupta) is a data-deficient small carnivore native to the central plains of North America that has experienced significant population declines. My dissertation investigates the species’ distribution, habitat associations, and interspecific interactions in South Dakota, providing crucial information for conservation actions. In Chapter 1, I developed ensemble distribution models on a broad scale, incorporating species location data from 1985–2020, environmental factors, and six predictive modeling algorithms. I identified key predictors including mean temperature diurnal range and proportion of area as pasture, and estimated ~31,300 km2 of potential habitat, predominately in eastern South Dakota. In Chapter 2, …
Utilizing Soil Sensors To Assess Soil Health And Investigating Cover Crops Impact On Methane Emission, Shailesh Pandit
Utilizing Soil Sensors To Assess Soil Health And Investigating Cover Crops Impact On Methane Emission, Shailesh Pandit
Electronic Theses and Dissertations
Soil health is influenced by climate change, cultural practices, topography, crop rotations, cover crops, soil parent materials, and soil biota. Interactions between these factors can improve or reduce soil health. This thesis investigates two topics, using soil sensors to provide a rapid assessment of soil health, and the impact of cover crops on soil biological activity and greenhouse gas emissions. Chapter 1 explores the use of two types of sensors to measure apparent electrical conductivity (ECa) and provides examples on the use of these sensors. Even though both sensors measure ECa using different processes, they provide useful information about temporal …
Evaluation Of A Novel Corn Fermented Protein Ingredient, Probiotics, And Postbiotics On Nursery Pig Growth Performance And Gut Health, Juan F. Castillo Zuniga
Evaluation Of A Novel Corn Fermented Protein Ingredient, Probiotics, And Postbiotics On Nursery Pig Growth Performance And Gut Health, Juan F. Castillo Zuniga
Electronic Theses and Dissertations
Weaning is a necessary step in pig production. After weaning, pigs are subjected to various nutritional, psychological, and environmental stresses. The weaning period is an important period for the intestinal development of the pig. During this time, digestion, immunity, metabolism, and various other aspects of the pig change rapidly. Therefore, providing high-quality feed ingredients is crucial to encourage the newly weaned pig to start consuming solid feed. Two independent studies were conducted to determine if the inclusion of: 1) corn fermented protein (CFP) or 2) probiotics or postbiotics impact nursery pigs' growth performance and gut health. The first experiment utilized …
Role Of Production Systems On Carcass And Palatability Attributes Of Bison, Lydia Margaret Hite O'Sullivan
Role Of Production Systems On Carcass And Palatability Attributes Of Bison, Lydia Margaret Hite O'Sullivan
Electronic Theses and Dissertations
The overall objective of this dissertation was to determine the impact of finishing systems on carcass and palatability attributes of bison. The specific objectives were 1) determine the influence of finishing system (grain-finished vs. grass-finished) on the mechanisms of tenderness formation in the longissimus lumborum of bison bulls, 2) determine the influence of diverse finishing systems on carcass characteristics and proximate composition of bison heifers, and 3) determine the influence of diverse finishing systems on palatability of striploin steaks from bison heifers. Results indicate that finishing systems differentially influence carcass characteristics, tenderness, and flavor attributes of bison. Specifically, this is …
Beef Hot Carcass Weight Influences Proteolysis, Clay J. Carlson
Beef Hot Carcass Weight Influences Proteolysis, Clay J. Carlson
Electronic Theses and Dissertations
The objective of this thesis was to determine the influence of beef hot carcass weight on proteolysis to better understand early postmortem proteolysis. Beef tenderness formation is influenced by various factors, such as temperature decline and proteolysis. Limited focus has been given to the effects of carcass weight on proteolysis. This study aimed to investigate the influence of increased beef hot carcass weight (HCW) on proteolysis of four muscles (serratus ventralis, longissimus thoracis, longissimus lumborum, and semitendinosus) during early postmortem aging times. Beef steers (n =12) were harvested and categorized into two weight groups: lightweight (LW; HCW = 349 ± …
Improving Stream Connectivity In South Dakota, Colton Rainier Curtis
Improving Stream Connectivity In South Dakota, Colton Rainier Curtis
Electronic Theses and Dissertations
Stream connectivity is crucial for fish movement and genetic diversity in fragmented landscapes. Road-crossings often act as barriers, disrupting hydrology and isolating fish populations. The Southeast Aquatic Resources Partnership (SARP) has developed rapid road-crossing assessment protocols and tools to prioritize restoration projects. However, managers lack tools for planning, directing, and initiating roadcrossing assessments. Managers also require modeling approaches to effectively identify and monitor road-crossings across dynamic stream networks. Presented here is a case study offering a roadmap for effective implementation of collaborative road-crossing assessments and prioritization of remediation projects, alongside an exploration of predictive modeling approaches to identify problematic crossings …
The Influence Of By-O-Reg+ Beef, An Oregano Essential Oil Supplement, On Growth Performance, Carcass Traits And Meat Quality Of Finishing Beef Steers, Bergin Mackenzie Debruin
The Influence Of By-O-Reg+ Beef, An Oregano Essential Oil Supplement, On Growth Performance, Carcass Traits And Meat Quality Of Finishing Beef Steers, Bergin Mackenzie Debruin
Electronic Theses and Dissertations
The objective of this thesis was to determine the influence of an oregano-based essential oil (OEO; By-O-Reg+ Beef), on growth performance, carcass traits, and meat quality of beef steers finished in an all-natural program. Yearling steers were allotted to 16 pens (n = 8 pens per treatment, 8 steers per pen). Treatments included: 1) control (CON) fed no oregano-based essential oil and 2) group fed 4 g/steer daily of By-O-Reg+ Beef. Steers were harvested on day 149 of the feeding period. Standard carcass data was collected and instrumental color (L*, a*, b*) was recorded. Strip loins were collected from a …
The Relationship Between Soil Test Phosphorus And Soil Biology - A Natural Management Of Soil Phosphorus Through Conservation Practices, Clarence Frederick Winter
The Relationship Between Soil Test Phosphorus And Soil Biology - A Natural Management Of Soil Phosphorus Through Conservation Practices, Clarence Frederick Winter
Electronic Theses and Dissertations
Phosphorus (P) management is an ongoing challenge in modern agriculture. Due to the importance of P in agriculture, P fertilizer additions are made throughout the U.S. to avoid nutrient-depleted soil. However, the application of P is problematic, as an excess of P in an agricultural system elevates the risk of environmental degradation, and current synthetic P sources are a fleeting resource. Additionally, a majority of P found in agricultural soils are insoluble, in the form of secondary and primary P minerals, making this large pool of P unavailable to plants. Fortunately, soil fungi organisms, such as arbuscular mycorrhizal fungi (AMF) …
Identification And Characterization Of Three Novel Rumen Bacterial Species In Sheep Fed A Concentrate Diet, Reid Frederick Anema
Identification And Characterization Of Three Novel Rumen Bacterial Species In Sheep Fed A Concentrate Diet, Reid Frederick Anema
Electronic Theses and Dissertations
Sheep production in the United States is a popular venture for both small farmers and large enterprise owners, as there is a market for end products such as wool and meat. One major factor of raising sheep is nutrition as an adequate diet will allow an animal to reach its full genetic potential for growth. With that in mind, the composition of the diet must be formulated to benefit the animal. Ruminants have the ability to use a variety of plants that are often inedible for humans to create products that humans can consume. These varying feedstuffs, whether they include …
Elucidating The Role Of Sialic Acid In Tumorigenic Pathways, Kakali Das
Elucidating The Role Of Sialic Acid In Tumorigenic Pathways, Kakali Das
Electronic Theses and Dissertations
Hypersialylation is a prognostic biomarker in cancer cells. The upregulated sialic acid expression on cancer cells facilitates tumorigenesis by playing a critical role in cancer cell proliferation and growth, immune evasion, cell signaling, and metastasis by interacting with various carbohydrate-binding molecules. Sialic acids on cancer cell surface undergo various modifications like O-acetylation at positions 4,7,9 de-acetylation and addition of glycolyl group at position 5. In our first chapter we analyzed the effect of de-acetylated sialic acid on migration via selectin binding in colon cancer cell line HCT116 and in lung cancer cell line A549. Selectins are calcium-dependent cell adhesion molecules …
Plant Pathways Associated With Competition For Soybean Root Colonization By Bradyrhizobium Strains, Athira Sethu Madhavan
Plant Pathways Associated With Competition For Soybean Root Colonization By Bradyrhizobium Strains, Athira Sethu Madhavan
Electronic Theses and Dissertations
Nitrogen is a crucial nutrient for the growth and development of plants. Legume crops like soybeans develop symbiotic associations with soil bacteria from the genus, Bradyrhizobium to obtain fixed nitrogen. In this mutualistic association, rhizobia are released into root nodules that provide a compatible environment for rhizobia to grow and fix nitrogen (Symbiotic Nitrogen Fixation (SNF)). A caveat in such beneficial associations is the competition between different rhizobium strains in soil for infection and nodule occupancy. The elite inoculant strains encounter competition by indigenous rhizobial population in the soil to infect and nodulate a susceptible legume host, because of which …
Ecophysiology Of Bradyrhizobium Diazoefficiens Usda 110, Armaan Kaur Sandhu
Ecophysiology Of Bradyrhizobium Diazoefficiens Usda 110, Armaan Kaur Sandhu
Electronic Theses and Dissertations
With increasing demand for soybean, maximizing plant yield is important yiel is dependent on nitrogen availability, but nitrogen fertilizers can meet the plant's needs but have severe environmental impacts. An economically and environmentally sustainable approach is to enhance nitrogen availability through Biological Nitrogen Fixation by symbiotic rhizobia, Bradyrhizobium, an efficient nitrogen fixer for soybean. This symbiosis begins with attachment of rhizobia to legume roots. Root and rhizobia surface properties play a significant role in this attachment. Another major factor affecting root colonization is bacterial competition ability. There is a lack of knowledge on the role of bacterial surface properties, competition …
Estimating South Dakota’S Capacity To Meet K-12 School Meal Fruit And Vegetable Requirements With State-Grown Produce, Bridgette Bienias
Estimating South Dakota’S Capacity To Meet K-12 School Meal Fruit And Vegetable Requirements With State-Grown Produce, Bridgette Bienias
Electronic Theses and Dissertations
Objective: Quantify South Dakota (SD)’s capacity to procure locally-sourced fruit and vegetables (FVs) for K-12 school meals.
Methods: Secondary analysis of 2017 SD agricultural census and school meal data. Yields were converted to edible cups of FVs using school meal guidelines provided by the U.S. Department of Agriculture. Student enrollment and meal redemptions were used to estimate the number of FV cups needed.
Results: Estimated SD FV production met minimum total K-12 cup requirements, except for total fruit (66%) and dark green vegetables (80%). Legumes supplied 77% of the total cups of vegetables produced in 2017, whereas dark green vegetables …
The Effects Of Temperature, Flooding, And Goose Feces Addition On Greenhouse Gas Emissions And Ammonification In Four High-Latitude Soils From Western Alaska, Jenna M. Ross
Electronic Theses and Dissertations
The large carbon (C) stock of wetlands is vulnerable to climate change, especially in high latitudes that are warming at a disproportional rate. Likewise, low-elevation Arctic coastal areas will flood more frequently under climate change and sea-level rise, which may alter goose herbivory and fecal deposition patterns if geese move inland. While temperature, flooding, and feces impact soil C emissions, their interactive effects have been rarely studied. Here, I explore the impact of these interactions on CO2 and CH4 emissions and nitrogen (N) mineralization (ammonification) in soils collected from four plant communities in the Yukon-Kuskokwim (Y-K) Delta, a high latitude …
Effects Of Storage Regimes On Chemistry, Functional Properties, And Vitamin Profile Of Different Varieties Of Chickpea, Shirin Kazemzadeh Pournaki
Effects Of Storage Regimes On Chemistry, Functional Properties, And Vitamin Profile Of Different Varieties Of Chickpea, Shirin Kazemzadeh Pournaki
Electronic Theses and Dissertations
Chickpea (cicer arietinum) samples from five distinct varieties (crown, royal, orion, sierra, and frontier) were subjected to varying storage conditions to investigate the impact of temperature and relative humidity (rh) on their chemical, functional, physical properties, and vitamin b profile. over 360 days, samples were stored at two temperatures (21°c and 40°c) and three rh levels (40%, 55%, 65%), with collections made every 90 days. initial observations focused on key chemical properties including moisture, protein, fat, and total starch content. subsequently, protein and starch fractions were isolated for further analysis, employing techniques such as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) …
Portable X-Ray Fluorescence Spectrometry For Sensing Salinity And Sodicity In Glacial Northern Great Plains Soils With Machine Learning Models, Adam Devlin
Electronic Theses and Dissertations
Saline and sodic soils are an increasing concern across the Northern Great Plains (NGP) due to factors of climate change and land management that are drawing geologically derived salts to the land surface. Traditional laboratory assessments, such as electrical conductivity (EC) and sodium adsorption ratio (SAR), are time and resource consumptive. Portable X-ray fluorescence (PXRF) may be a viable proximal sensing alternative, as it is able to provide elemental data in minutes, in situ or ex situ, and can directly quantify salinity-associated elements like Ca, Mg, and S. PXRF paired with predictive models has proven useful for a range of …
The Relationship Of Macronutrient Intake With Growth In Children With Type 1 Diabetes Mellitus, Jessica L. Freeling
The Relationship Of Macronutrient Intake With Growth In Children With Type 1 Diabetes Mellitus, Jessica L. Freeling
Electronic Theses and Dissertations
Child growth, a sensitive metric of overall health, results from the intricate interplay of nature and nurture. While the importance of nutrition in child growth is well established, growth trajectories exhibit substantial individual variability, influenced by sex and age, and often characterized by nonlinear patterns. Inadequate nutrition or disease can hinder growth, with potential for recovery upon proper nutrition or acute disease resolution. However, chronic disease or persistent malnutrition may lead to permanent growth perturbation. Notably, before the advent of insulin, chronic stunting and wasting were hallmarks of Type 1 diabetes mellitus (T1DM), though the specific impacts under modern standard …
Longevity In Afrikaner Cattle: A Survival Analysis, Allison M. Bot Steffl
Longevity In Afrikaner Cattle: A Survival Analysis, Allison M. Bot Steffl
Electronic Theses and Dissertations
The Afrikaner breed of cattle is indigenous to South Africa and due to their hardiness was once the most popular breed amongst farmers. The objectives for this study were to: (1) estimate environmental effects (age at first calving, season) and (2) genetic variance affecting longevity in Afrikaner cattle using survival analysis and a Weibull model and (3) evaluate the effect of the Infusion Project on longevity utilizing a Cox model. For the first objective, age at first calving impacted longevity as younger age at first calving was associated with decreased longevity. Season of birth and year of birth also impacted …
Evaluation Of Dietary Fiber Technologies In Sow Diets, Garrin Shipman
Evaluation Of Dietary Fiber Technologies In Sow Diets, Garrin Shipman
Electronic Theses and Dissertations
Various technologies and alternative ingredients are being developed to increase the nutrient value of fibrous feedstuffs, improve animal performance, and promote sustainable swine production. Fiber-degrading enzymes have been extensively studied in growing pigs, with few studies focused on gestating sows. When considering gestating sows have greater energy digestibility of fibrous feedstuffs than grower pigs, the efficacy of fiber-degrading enzymes needs to be determined for implementation in commercial sow diets. As fiber inclusion in gestation diets benefits sow performance, corn dried distiller grains with solubles (DDGS) are a candidate fiber source for gestation alternative energy ingredient. Functional fibers, such as yeast …
Influence Of Reduced Salt In Fully Cooked Ham And Sausage Cured With Natural Celery Powder On The Growth Of Listeria Monocytogenes At 4⁰C, Anne Sherod
Electronic Theses and Dissertations
This research evaluated the influence of reducing salt on outgrowth of L. monocytogenes on ready to eat (RTE) deli-style sliced ham cured with natural celery powder, fully cooked sausage links cured with traditional sodium nitrite and sausage links cured with natural celery powder, vacuum packaged and stored at 4⁰C on the outgrowth of L. monocytogenes. Experiment 1 evaluated L. monocytogenes outgrowth on ham over 153 days on the following treatments, current retail ham (HC) 2.0% salt; reduced salt 1 (HRS1) 1.5% salt; reduced salt 2 (HSR2) 1.35% salt; and reduced salt 2 without antimicrobials (HRS2WA) 1.35% salt without potassium lactate …
Development Of Innovative Flocculation Technologies For Agricultural Water Treatment, Noor Haleem
Development Of Innovative Flocculation Technologies For Agricultural Water Treatment, Noor Haleem
Electronic Theses and Dissertations
The development of innovative flocculation technologies is essential for addressing the challenges of agricultural water treatment. These technologies play a crucial role in removing contaminants such as suspended solids and nutrients, thereby ensuring safer water for irrigation and livestock consumption. By enhancing water quality and wastewater management, they contribute significantly to environmental sustainability and public health in agricultural communities. This comprehensive thesis extensively explores various dimensions of flocculation, with a focused effort on methodologies and resources aimed at strengthening sustainability and efficiency. A pivotal aspect of the research involves synthesizing cationic starch (CS), a flocculant derived from an underutilized resource, …
Developing Machine Learning Models For Selection Of Management Zones, Sravanthi Bachina
Developing Machine Learning Models For Selection Of Management Zones, Sravanthi Bachina
Electronic Theses and Dissertations
Soil sampling and analyses play a crucial role in optimizing nutrient management and enhancing crop productivity. However, collecting representative samples across diverse landscapes is challenging due to knowledge gaps about spatial variability of soil properties, large fields, multiple samples, and analysis costs. Collecting soil samples based on the management zones can help farmers gather precise information about soil properties with fewer samples. Recent developments in precision agriculture and machine learning. This study aimed to develop machine learning models that can learn, analyze, and refine landscape and soil properties data for automated selection of soil sampling zones and generating prediction maps. …