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The Rna-Binding Site Of Bacteriophage Qβ Coat Protein, Francis Lim, Marc Spingola, David S. Peabody Dec 1996

The Rna-Binding Site Of Bacteriophage Qβ Coat Protein, Francis Lim, Marc Spingola, David S. Peabody

Marc Spingola

The coat proteins of the RNA bacteriophages Qβ and MS2 are specific RNA binding proteins. Although they possess common tertiary structures, they bind different RNA stem loops and thus provide useful models of specific protein-RNA recognition. Although the RNA-binding site of MS2 coat protein has been extensively characterized previously, little is known about Qβ. Here we describe the isolation of mutants that define the RNA-binding site of Qβ coat protein, showing that, as with MS2, it resides on the surface of a large β-sheet. Mutations are also described that convert Qβ coat protein to the RNA binding specificity of MS2. …


Taxonomic Status Of The Enigmatic Cryptotis Avia (Mammalia: Insectivora: Soricidae), With Comments On The Distribution Of The Colombian Small-Eared Shrew, Cryptotis Colombiana, Neal Woodman Oct 1996

Taxonomic Status Of The Enigmatic Cryptotis Avia (Mammalia: Insectivora: Soricidae), With Comments On The Distribution Of The Colombian Small-Eared Shrew, Cryptotis Colombiana, Neal Woodman

Neal Woodman

Cryptotis avia G. M. Allen 1823, from the Eastern Andean Cordillera of Colombia, is a small-eared shrew previously known from, at most, five specimens. Review of the holotype, consisting of an understuffed skin and a partial skull, and three potential topotypes indicates that they are refereable to Cryptotis thomasi Merriam, 1897, and C. avia should be considered ajunior synonym of that name. Cryptotis thomasi is restricted to highlands around Bogotá in the Eastern Cordillera. Another specimen previously referred to C. avia is the second known specimen of Cryptotis colombiana Woodman & Timm 1993. This second record expands the known distribution …


Emergence And Overwintering Brood Of Douglas-Fir Beetle Seven Years After The Clover Mist Fire On The Clarks Fork Ranger District, Shoshone National Forest, Wyoming, Judith E. Pasek Aug 1996

Emergence And Overwintering Brood Of Douglas-Fir Beetle Seven Years After The Clover Mist Fire On The Clarks Fork Ranger District, Shoshone National Forest, Wyoming, Judith E. Pasek

Judith E Pasek

Emergence patterns and population levels of Douglas-fir beetles (DFB), Dendroctonus pseudotsugae Hopkins, in the seventh year of infestation following the Clover Mist Fire were evaluated on the Clarks Fork Ranger District of the Shoshone National Forest. Adult emergence in 1995 returned to a single peak beginning in mid-June, about a week later than normal likely due to cold, wet spring weather. Adults of both the '93-'94 and '94-'95 generations emerged, demonstrating for the second year in a row that DFB can extend its life cycle up to two years. Almost half of the total emergence of the '93-'94 generation was …


Improved Cultivation Systems For Isolation Of The Colorado Potato Beetle Spiroplasma., Meghnad Konai, K. J. Hackett, D. L. Williamson, J. J. Lipa, J. D. Pollack, G. E. Gasparich, E. A. Clark, D. C. Vacek, R. F. Whitcomb Aug 1996

Improved Cultivation Systems For Isolation Of The Colorado Potato Beetle Spiroplasma., Meghnad Konai, K. J. Hackett, D. L. Williamson, J. J. Lipa, J. D. Pollack, G. E. Gasparich, E. A. Clark, D. C. Vacek, R. F. Whitcomb

Gail Gasparich

In North America, the Colorado potato beetle, Leptinotarsa decemlineata, is often infected with the host-specific, gut-inhabiting Colorado potato beetle spiroplasma (CPBS). CPBS is apparently a commensal, but it may be useful in biocontrol if it can be transformed to express an insect-lethal gene. Difficulty in cultivating the organism, however, has hindered the development of a suitable transformation system. In this study, we eliminated the need for coculturing CPBS with insect cells. CPBS was reliably isolated with the BBL Anaerobic GasPak Jar system (low redox, enhanced CO(inf2)), which was easier to use and less expensive than insect cell coculture methods. A …


Pollen Dispersal In Low-Density Populations Of Three Neotropical Tree Species, E. A. Stacy, J. L. Hamrick, J. D. Nason, S. P. Hubbell, R. B. Foster, R. Condit Jul 1996

Pollen Dispersal In Low-Density Populations Of Three Neotropical Tree Species, E. A. Stacy, J. L. Hamrick, J. D. Nason, S. P. Hubbell, R. B. Foster, R. Condit

John Nason

Studies of mating patterns of tropical trees, typically involving common species, have revealed that most species are outcrossed and that, in some cases, a significant fraction of outcross pollen moves long distances. We evaluated mating systems and effective pollen dispersal for three hermaphroditic, insect-pollinated Neotropical tree species, Calophyllum longifolium, Spondias mombin, and Turpinia occidentalis, all of which occurred at low adult densities at the study site. Mating patterns were estimated for each maternal tree within 84-ha populations of C. longifolium and S. mombin in 1992 and 1993 and within a 50-ha population of T. occidentalis in 1993. Each population was …


Developmental And Tissue-Specific Expression Of Mouse Pelle-Like Protein Kinase, Marina Trofimova, Amy B. Sprenkle, Melissa Green, Thomas W. Sturgill, Mark G. Goebl, Maureen A. Harrington Jul 1996

Developmental And Tissue-Specific Expression Of Mouse Pelle-Like Protein Kinase, Marina Trofimova, Amy B. Sprenkle, Melissa Green, Thomas W. Sturgill, Mark G. Goebl, Maureen A. Harrington

Amy Sprenkle

The NF-kappaB/c-Rel proteins are a family of evolutionarily conserved transcription factors activated during development that in the adult, mediate many processes including the immune response. A high degree of sequence similarity is shared between the NF-kappaB/c-Rel family of transcription factors and the Drosophila Dorsal protein as well as between its cytoplasmic inhibitor, IkappaBalpha, and the Drosophila Cactus protein. Genetic analyses of Dorsal have defined components of a signaling pathway for Dorsal activation, including a serine/threonine kinase, Pelle, placed upstream of Dorsal and Cactus. We demonstrate that this pathway is likely to be conserved in mammals by the isolation of a …


Spatial Distribution And Sequential Sampling Plans For The Banded Sunflower Moth (Lepidoptera: Cochylidae) Eggs On Sunflower, C Peng, Gary Brewer Dec 1995

Spatial Distribution And Sequential Sampling Plans For The Banded Sunflower Moth (Lepidoptera: Cochylidae) Eggs On Sunflower, C Peng, Gary Brewer

Gary J. Brewer

No abstract provided.


Natural Selection And Self-Organization: Dynamical Models As Clues To A New Evolutionary Synthesis, Bruce Weber, David Depew Dec 1995

Natural Selection And Self-Organization: Dynamical Models As Clues To A New Evolutionary Synthesis, Bruce Weber, David Depew

David J Depew

The Darwinian concept of natural selection was conceived within a set of Newtonianbackground assumptions about systems dynamics. Mendelian genetics at first did not sit well with the gradualist assumptions of the Darwinian theory. Eventually, however. Mendelism and Darwinism were fused by reformulating natural selection in statistical terms. This reflected a shift to a more probabilistic set of background assumptions based upon Boltzmannian systems dynamics. Recent developments in molecular genetics and paleontology have put pressure on Darwinism once again. Current work on self-organizing systems may provide a stimulus not only for increased problem solving within the Darwinian tradition, especially with respect …


Montandon Marsh: A Vegetation Description Of A Potentially Endangered Wetland, E. Hochman, W. Abrahamson, J. Clark Dec 1995

Montandon Marsh: A Vegetation Description Of A Potentially Endangered Wetland, E. Hochman, W. Abrahamson, J. Clark

Warren G. Abrahamson, II

No abstract provided.


Cartoons About Science.Pdf, Mark Coyne Dec 1995

Cartoons About Science.Pdf, Mark Coyne

Mark Coyne

A tongue-in-cheek look at the world of Natural Science.


So You Want To Be A Soil Microbiologist.Pdf, Mark Coyne Dec 1995

So You Want To Be A Soil Microbiologist.Pdf, Mark Coyne

Mark Coyne

A tongue-in-cheek look at soil microbiology.


In-Situ Imaging Of Ionic Crystal Dissolution Using An Integrated Electrochemical/Afm Probe, Julie V. Macpherson, Patrick R. Unwin, Andrew C. Hillier, Allen J. Bard Dec 1995

In-Situ Imaging Of Ionic Crystal Dissolution Using An Integrated Electrochemical/Afm Probe, Julie V. Macpherson, Patrick R. Unwin, Andrew C. Hillier, Allen J. Bard

Andrew C. Hillier

The kinetics and mechanism controlling dissolution from the (100) cleavage face of potassium bromide single crystals in acetonitrile solutions have been identified using a novel integrated electrochemical/AFM probe and a scanning electrochemical microscope (SECM). With both techniques, dissolution is induced by perturbing the dynamic dissolution/growth equilibrium at the crystal/solution interface through the electrochemical oxidation of bromide ions. SECM measurements demonstrate that the dissolution reaction is diffusion-limited under the experimental conditions, suggesting that the surface reaction is characterized by a rate constant in excess of 5 cm s-1 (assuming a first-order dissolution process). The topography of the dissolving surface has been …


Homology Among Nearly All Plasmids Infecting Three Bacillus Species, Piotr Zawadzki, Margaret A. Riley, Frederick M. Cohan Dec 1995

Homology Among Nearly All Plasmids Infecting Three Bacillus Species, Piotr Zawadzki, Margaret A. Riley, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.


Assessment And Decision Making In Animals: A Mechanistic Model Underlying Behavioral Flexibility Can Prevent Ambiguity, Amos Bouskila, Daniel T. Blumstein Dec 1995

Assessment And Decision Making In Animals: A Mechanistic Model Underlying Behavioral Flexibility Can Prevent Ambiguity, Amos Bouskila, Daniel T. Blumstein

Amos Bouskila

Understanding how animals make decisions is a fundamental question in behavioral ecology which has cascading effects on how animals respond to environmental variation. An explicit model of the mechanisms of information processing and decision making can help prevent conflated definitions and ambiguous interpretations. Unambiguous definitions are crucial for clear communication between theoreticians and empiricists and for the rapid advancement of studies of decision making, Moreover, employing a clear model of underlying proximal processes will help bridge the gap between cognitive psychology and behavioral ecology and should aid scientific advancement. We present a simple model to guide studies of assessment and …


Food-Limited Growth And Condition Index In The Eastern Oyster, Crassostrea Virginica (Gmelin, 1791), And The Bay Scallop, Argopecten Irradians Irradians (Lamarck, 1819), Michael A. Rice, Robert B. Rheault Jr. Dec 1995

Food-Limited Growth And Condition Index In The Eastern Oyster, Crassostrea Virginica (Gmelin, 1791), And The Bay Scallop, Argopecten Irradians Irradians (Lamarck, 1819), Michael A. Rice, Robert B. Rheault Jr.

Michael A Rice

The growth response of the eastern oyster, Crassostrea virginica, and the bay scallop, Argopecten irradians irradians, to varying degrees of food limitation was evaluated. Under conditions of low current speed, dense assemblages of shellfish can rapidly deplete ambient food concentrations, resulting in measurable effects on growth and condition index. A flume study demonstrated significant growth and condition index responses to resource competition after reductions as small as 27% in relatively high ambient food concentrations (—4.6 u.g/1 chlorophyll). Growth rates and condition index are linearly correlated with the average chlorophyll ration consumed. A field study demonstrated similar growth responses when the …


A Survey Of Disease In The Oyster Crassostrea Virginica (Gmelin, 1791) In Rhode Island Coastal Estuaries, Retno Andamari, Michael A. Rice, Paul P. Yevich Dec 1995

A Survey Of Disease In The Oyster Crassostrea Virginica (Gmelin, 1791) In Rhode Island Coastal Estuaries, Retno Andamari, Michael A. Rice, Paul P. Yevich

Michael A Rice

The presence of potential pathogens and lesions in American oysters, Crassostrea virginica (Gmelin, 1791), from coastal estuaries in Rhode Island was studied. Oysters were collected by hand or tongs from three stations in the Pawcatuck River, two stations in Narrow River, three stations in Charlestown Pond, and three stations in Green Hill Pond, during July/August 1991, November 1991, March 1992, and May 1992. Oysters were processed for histologic examination and determination of condition index. MSX, Haplosporidium nelsoni (Haskin, Stauber et Mackin, 1966), was detected in four of 480 oysters (0.8%); Sphenophyra sp., 15 of 480 (3.1%); Bucephalus sp. 16of 480 …


Brain Activation Modulated By Sentence Comprehension, Marcel Adam Just, Patricia A. Carpenter, Timothy A. Keller, William F. Eddy, Keith R. Thulborn Dec 1995

Brain Activation Modulated By Sentence Comprehension, Marcel Adam Just, Patricia A. Carpenter, Timothy A. Keller, William F. Eddy, Keith R. Thulborn

Marcel Adam Just

No abstract provided.


Measurement Of Double-Layer Forces At The Electrode/Electrolyte Interface Using The Atomic Force Microscope: Potential And Anion Dependent Interactions, Andrew C. Hillier, Sunghyun Kim, Allen J. Bard Dec 1995

Measurement Of Double-Layer Forces At The Electrode/Electrolyte Interface Using The Atomic Force Microscope: Potential And Anion Dependent Interactions, Andrew C. Hillier, Sunghyun Kim, Allen J. Bard

Andrew C. Hillier

The forces between a silica probe and silica and gold substrates were measured with an atomic force microscope in the presence of a series of alkali-halide electrolyte solutions. The interaction between two silica surfaces was repulsive and could be accurately predicted by Derjaguin-Landau-Verwey-Overbeek theory. The silica surface was negatively charged at a pH of 5.5 and the effective surface potential increased in magnitude with decreasing electrolyte concentration. In contrast, the force between the silica probe and a gold substrate was attractive at open circuit. This interaction was a strong function of the potential applied to the gold and the nature …


The Potential For Ontogenetic Vertical Migration By Larvae Of Bathyal Echinoderms, William Jaeckle, Craig M. Young, Michael G. Devin, Suki U.K. Ekaratne, Sophie B. George Dec 1995

The Potential For Ontogenetic Vertical Migration By Larvae Of Bathyal Echinoderms, William Jaeckle, Craig M. Young, Michael G. Devin, Suki U.K. Ekaratne, Sophie B. George

William Jaeckle

Planktotrophy is a relatively common developmental mode among bathyal and abyssal echinoderms, but the sources of food used by deep-sea planktotrophic larvae remain generally unknown. Very few deep-sea echinoderm larvae have been collected in plankton samples, so we do not know whether larvae migrate to the euphotic zone to feed or if they rely on bacteria or detritus at greater depths. We approached this question indirectly by investigating whether larvae of bathyal echinoids can tolerate the temperatures they would encounter in the euphotic zone and whether they possess sufficient energy stores to migrate to the euphotic zone without feeding. Twenty-four …


Trigeminal Repellents Do Not Promote Conditioned Odor Avoidance In European Starlings, Larry Clark Dec 1995

Trigeminal Repellents Do Not Promote Conditioned Odor Avoidance In European Starlings, Larry Clark

Larry Clark

Birds, and in particular European Starlings (Stumus vulgaris), avoid con­ sumption of fluid and food treated with the natural plant products, methyl anthranilate and o-aminoacetophenone. Avoidance is an unlearnedresponse most likely mediated via chem­ ically sensitive fibers of the trigeminal nerve. The trigeminal nerve codes for chemical irritation and pain. Starlings are not repelled by the odor of the compounds, nor is olfaction important in the avoidance response. Moreover, starlings fail to learn to avoid the odor of the repellents, even after direct oral contact with liquid repellent. While trigeminal irritants can be powerful repellents, the absence of associative learning …


Population Genetics And Gene Variation In Screwworm From Brazil (Diptera: Calliphoridae)., David B. Taylor, Richard D. Peterson Ii, Gonzalo E. Moya-Borja Dec 1995

Population Genetics And Gene Variation In Screwworm From Brazil (Diptera: Calliphoridae)., David B. Taylor, Richard D. Peterson Ii, Gonzalo E. Moya-Borja

David B. Taylor

Allozyme variation in New World screwworm, Cochliomyia hominivorax (Coquerel), populations from Brazil was examined. Variability was observed in 8 of 13 enzyme loci and the frequency of the most common allele was <0.95 for seven loci. Observed and expected heterozygosities were 0.159 and 0.165, respectively. Comparisons of the Brazilian populations with previously studied populations from Costa Rica resulted in Nei's genetic distances of between 0.000 and 0.006, with the greatest distance being between populations within Brazil. Comparisons with Mexican populations using only three loci resulted in genetic distances <0.031. Goodness-of-fit statistics for Hardy–Weinberg equilibrium and Wright's F statistics indicated small deviations from expected equilibrium genotype frequencies and low levels of differentiation between populations within Brazil. Differentiation among screwworm populations from Brazil, Costa Rica, and Mexico was minimal.


Concaveation And Maintenance Of Maternal Behavior In A Burrower Bug (Sehirus Cinctus): A Comparative Perspective, Scott Kight Dec 1995

Concaveation And Maintenance Of Maternal Behavior In A Burrower Bug (Sehirus Cinctus): A Comparative Perspective, Scott Kight

Scott Kight

This study investigates 2 patterns of maternal behavior typical of mammals, using a heterop- teran insect as the study animal. Sehirus cinctus, a burrower bug (Heteroptera: Cydnidae), exhibits relatively well-developed maternal behavior that includes guarding eggs and provi- sioning offspring. Mothers remained maternally responsive to stimulus eggs for 24-48 hr following removal of their own eggs, but the response grew weaker with longer separation times. A proportion of nulliparous females also exhibited maternal responsiveness when presented with stimulus eggs. Males, however, never responded parentally to eggs. The results of this study highlight similarities in general female responsiveness to stimulus young …


Mapping The Space Of Time: Temporal Representation In The Historical Sciences, Robert J. O’Hara Dec 1995

Mapping The Space Of Time: Temporal Representation In The Historical Sciences, Robert J. O’Hara

Robert J. O’Hara

William Whewell (1794–1866), polymathic Victorian scientist, philosopher, historian, and educator, was one of the great neologists of the nineteenth century. Although Whewell’s name is little remembered today except by professional historians and philosophers of science, researchers in many scientific fields work each day in a world that Whewell named. “Miocene” and “Pliocene,” “uniformitarian” and “catastrophist,” “anode” and “cathode,” even the word “scientist” itself—all of these were Whewell coinages. Whewell is particularly important to students of the historical sciences for another word he coined, one that was unfortunately not as successful as many of his others because it is difficult to …