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- Biology (13)
- Science (2)
- Aethina tumida; small hive beetle; haplotyping; apis mellifera (1)
- Amyotrophic Lateral Sclerosi; ALS; neurodegenerative disease; protein aggregation; Cu/Zn Superoxide dismutase 1; SOD1; Drosophilia; (1)
- Amyotrophic lateral sclerosis; ALS; superoxide dimutase 1; Drosophila Melanogaster; protein aggregation; (1)
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- Biochemistry; FRET; polymerase; DNA repair; Fluorescence Resonance Energy Transfer; FRET; (1)
- Biology; Biochemistry; Chemistry; Tau;Protein (1)
- Biology; Neuroscience; hypoxia; ischemia; anti-inflammatory; brain (1)
- Biotechnology (1)
- Clearcutting; Ecosystem function; Biogeochemical; New England; Forest; Legacy effects; Carbon sink; Extracellular enzymes; ecoenzymes (1)
- Genetic engineering; college student viewpoints on genetic engineering; college students; assisted reproductive technology; mass media; ethics; Rhode Island College; Weber’s value-rational action theory; Bourdieu’s theory of capital; (1)
- Hypoxia-ischemia; brain development; neuronal plasticity; (1)
- Intraspecific variability; rushia intricata; aridari; noctiflorum; diospyros austro-africana; chrysocoma ciliata; (1)
- Iridophores; zebrafish; developmental biology; mitfa; GRN (1)
- Lepomis macrochirus; dorsal fin; median fin; innervation; bluegill; AAT; CGRP; CK20; immunohistology; immunohistochemistry; biomechanics; locomotion; proprioception (1)
- Molecular biology; biochemistry; protein aggregation; Tau; neurodegeneration; post-translational modification; (1)
- Molecular biology;PCR technology;polyhydroxyalkanoic acid;PHA;Vibrio; B-18;bacteria;PHA synthesis;antibiotic sensitivity;DNA sequences;Escherichia coli plasmids;electroporation (1)
- Muscle activity; pertubation; motor control; motor impairment; electromyography; fish (1)
- Myogenic regulatory factors; ciona intestinalis; invertebrate; muscle development; (1)
- Natural resources (1)
- Neurodegenerative diseases; Tau; (1)
- Public policy (1)
- Rat studies; laboratory studies; neonatal; encephalopathy; (1)
- Runway replication; plasmid; plasmid pJM9487; vibrio species b-18; polyhydroxyalkanoates; gene replication; temperature; (1)
- Social research (1)
- Sociology (1)
- Ssal; Hsp70; N-terminal acetylation; PTMs; protein purification; Hsp40; Molecular chaperones; Ydj1; Post-translational modification; chaperone modification; one-step purification; (1)
- Tree species;tree height;canopy trees;New England forests;tree growth;forest gap;Acer saccharum;sugar maple trees; Yale-Myers Experimental Forest; Quercus rubra; red oak trees;Acer rubrum; red maple trees (1)
- Ufd2a; VCP/p97; IBMPFD (1)
- Zebra danio;Ubiquitin;UFD2A transcripts;zebrafish;embryology;muscle development (1)
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Dorsal Fin Innervation Of The Bluegill, Lepomis Macrochirus, Amanda Torick
Dorsal Fin Innervation Of The Bluegill, Lepomis Macrochirus, Amanda Torick
Honors Projects
Recent work has shown that the dorsal fins in fish operate not only as locomotive appendages, but also as sensory structures that aid in proprioception. To investigate how fish use these structures for sensing, the innervation of the dorsal fins in bluegill sunfish (Lepomis macrochirus) was assessed through immunohistochemistry assays. I conducted staining using anti-acetylated tubulin (AAT), a non-specific neuron marker, calcitonin-gene related peptide (CGRP) which stains sensory neurons and cytokeratin 20 (CK20), which binds to mechanoreceptor structures. Stained samples of bluegill dorsal fins were imaged under a laser scanning epifluorescence microscope to visualize mechanoreceptors, as well as sensory and …
Legacy Effects Of Forest Clearcutting On Ecosystem Function And Biogeochemical Cycling In A New England Forest, Andre Solomon Gomes
Legacy Effects Of Forest Clearcutting On Ecosystem Function And Biogeochemical Cycling In A New England Forest, Andre Solomon Gomes
Honors Projects
Terrestrial soils comprise the largest reservoirs of carbon (C) in the biosphere holding some 2500 Gt C, which is 4x the amount found in plant biomass and 3x the C content of the atmospheric pool. Approximately 1500 Gt of soil C is in an organic form, thus making it available to mineralization by heterotrophic organisms [10]. Changes to the drivers of mineralization such as climate change and aeration due to anthropogenic land use, such as logging, have the potential to strongly affect global carbon and associated nutrient cycles, plant production, and atmospheric composition. With the aim of better understanding the …
Understanding The Effects Of N-Terminal Acetylation On The Oligomeric State Of Tau, Miguel Jose Martinez Guzman
Understanding The Effects Of N-Terminal Acetylation On The Oligomeric State Of Tau, Miguel Jose Martinez Guzman
Honors Projects
Protein production, purification, and analysis done in order to find the effects of N-terminal acetylation on microtubule associated protein tau.
Expression And Purification Of N-Terminally Acetylated Microtubule Binding Protein Tau, Abigail Fleurima
Expression And Purification Of N-Terminally Acetylated Microtubule Binding Protein Tau, Abigail Fleurima
Honors Projects
The microtubule-associated protein Tau (MAPT) plays a critical role in many neurodegenerative diseases. Tau functions to stabilize microtubule structures that are essential for transport within the neuron, and transport disruption leads to loss of neuronal function. Tau binding is regulated by phosphorylation with the help of kinases that add phosphate groups which block microtubule binding sites, and phosphatases that remove phosphate groups and expose the microtubule-binding regions. Tau can be found in hyper-phosphorylated states, which causes Tau to self-assemble into aggregates and prevent microtubule binding. It's clear that post-translational modifications (PTMs) of Tau play a key role in the dysregulation …
Function Of Spiny Dorsal Fin Erector Muscles In The Bluegill, Lepomis Macrochirus, Zakiyat Djabakatie
Function Of Spiny Dorsal Fin Erector Muscles In The Bluegill, Lepomis Macrochirus, Zakiyat Djabakatie
Honors Projects
Local motor control directly contributes to stability, which can be compromised by injury or multiple neuromuscular disorders. In addition, lack of sensory perception as experienced by decreased limb sensation can further deteriorate one‚Äôs quality of life. The goal of this study is to use bluegill (Lepomis macrochirus) fins as model systems to study and gain insights on local motor control and sensory perception to improve stability and locomotion in humans, especially in a rehabilitative state. We hypothesize that 1) when exposed to turbulence (T), bluegill will use the spiny dorsal fin to recover stability and muscle intensity and duration will …
Investigating The Role Of Alx4a In Mitfa Repression And Iridophore Fate Biasing, Melanie Cragan
Investigating The Role Of Alx4a In Mitfa Repression And Iridophore Fate Biasing, Melanie Cragan
Honors Projects
Investigating the role of the transcription factor alx4a on chromatophore fating in zebrafish.
Detecting Aggregated Superoxide-Dismutase Protein Using Aggregate Specific Antibodies, Helen Magana
Detecting Aggregated Superoxide-Dismutase Protein Using Aggregate Specific Antibodies, Helen Magana
Honors Projects
Amyotrophic lateral sclerosis is neurodegenerative disease characterized by late-onset, loss of motor neurons, paralysis, and eventual death. SOD1 is a free radical scavenger that normally resides in the cytoplasm, nucleus, and intermembrane space of the mitochondria. Mutations in SOD1, a ubiquitously expressed free-radical scavenger, are one cause of ALS. Degeneration of motor neurons is thought to be triggered by aggregation of mutant SOD1 followed by propagation to adjacent cells though cell-cell contacts. Here, we present immunohistochemical findings on tissues of a Drosophila melanogaster expressing mutant dsod1 alleles at the endogenous locus. Aggregate-specific antibodies were used to assess the presence of …
The Purification And Characterization Of N-Terminally Acetylated Ssal From Saccharomyces Cerevisiae, Alijah A. Griffith
The Purification And Characterization Of N-Terminally Acetylated Ssal From Saccharomyces Cerevisiae, Alijah A. Griffith
Honors Projects
Molecular chaperones are highly conserved, ubiquitous enzymes that maintain proteostasis by mediating protein structure in vivo. Post-translational modifications (PTMs) can act as a functional switch between chaperone pathways, allowing for cells to enact a coordinated response to cellular perturbations. Several PTMs are well characterized in the context of chaperone function, though the role of N-terminal acetylation is commonly overlooked, leading to an overwhelming lack of scientific understanding in this area of study. Ssa1, a Hsp70 class chaperone endogenous to Saccharomyces cerevisiae, is likely N-terminally acetylated, though in vitro studies of Ssa1 are limited by current purification methods which are resource-intensive. …
Building An In Vivo Model Of Tau Aggregation In Saccharomyces Cerevisae, Jessica Anderson
Building An In Vivo Model Of Tau Aggregation In Saccharomyces Cerevisae, Jessica Anderson
Honors Projects
Protein aggregates, which can result in cellular toxicity, are found in the brains of people afflicted with neurodegenerative diseases. One of the proteins found to aggregate is Tau, a protein that stabilizes microtubules in neuronal cells. Based on its amino acid sequence, Tau is likely to be N-terminally acetylated by the complex NatA, which is responsible for the acetylation of a majority of proteins with this post-translational modification. Mutations in the NatA complex result in a variety of pleiotropic detrimental phenotypes, showing that acetylation is a crucial modification for many proteins. Without the presence of NatA – and therefore without …
Monitoring Of Dna Polymerase Theta Movements Through Fret Analysis, Ashley Rebelo
Monitoring Of Dna Polymerase Theta Movements Through Fret Analysis, Ashley Rebelo
Honors Projects
DNA polymerases are enzymes used for DNA replication during cell division and can be specialized for DNA repair. DNA Polymerase Theta (Pol θ) is the predominant polymerase involved in alternative double-stranded break repair and is upregulated in breast cancer. It is errorprone as it does not accurately match the nucleotide on a DNA template with the correct complementary base. This inaccuracy affects the overall fidelity of the enzyme, a biochemical process that looks at the ability of a polymerase to “read” the template DNA and select the right nucleotide before polymerization. Polymerization for most DNA polymerases involves a global conformational …
Haplotyping Small Hive Beetle (Aethina Tumida) Reveals Na1 And Na2 Distribution In Rhode Island Apis Mellifera Hives, Katelyn St. George
Haplotyping Small Hive Beetle (Aethina Tumida) Reveals Na1 And Na2 Distribution In Rhode Island Apis Mellifera Hives, Katelyn St. George
Honors Projects
The small hive beetle (Aethina tumida) is a parasite of honeybee hives (Apis mellifera) and native to South Africa. Invasive in North America since 1996, the species has spread to hives throughout the continent, including many in Rhode Island and nearby states. To better understand migration patterns for this invasive species, we haplotyped small hive beetles (SHB) based on mitochondrial Cytochrome Oxidase Part 1 (COI) gene sequences. To analyze spatial and temporal haplotype distribution, we mapped haplotype data along with previously analyzed population numbers using ArcGIS. All identified beetles fell into one of two previously described haplotypes, either NA1 or …
Creating A Drosophila Sod1 Mutant Through Homologous Recombination, Saman Nayyab Stilwell
Creating A Drosophila Sod1 Mutant Through Homologous Recombination, Saman Nayyab Stilwell
Honors Projects
This works describes the creation and partial phenotypic analysis of a point mutation within the endogenous Drosophila sod gene. The sodS111C allele was generated through a process of mutagenesis and homologous recombination in vivo. SodS111C stocks were created to analyze the mutation’s influence on protein aggregation, if any. We were able to test the effects of this mutant by assessing the function of the protein using genetic assays. To examine the effect of sodS111C on the second chromosome in the presence of sodG85R on the third chromosome appropriate genetic crosses were performed and it was determined that there was no …
Cimrf Suppression Of Endogenous Tissue Developments In Ciona Intestinalis Embryos, Emmanuel Kofi Asieidu
Cimrf Suppression Of Endogenous Tissue Developments In Ciona Intestinalis Embryos, Emmanuel Kofi Asieidu
Honors Projects
Trans-differentiation of non-muscle tissue to muscle tissue is a well-established property of vertebrate myogenic regulatory factors (MRFs). For instance the vertebrate MRF, MyoD, has been shown to convert nonmuscle cells to muscle cells. During this trans-differentiation, however, endogenous gene activity of the nonmuscle cells is down regulated. To test if this property is also found in invertebrate MRFs our lab has developed a system to analyze the ability of the MRF of Ciona intestinalis, CiMRF, to suppress endogenous gene activity during trans-differentiation. We did this by misexpressing CiMRF in the notochord and endoderm of Ciona embryos, two nonmuscle …
Intraspecific Variation In Leaf Traits Across An Environmental Gradient In The Cape Floristic Provence Of South Africa, Bruce H. Ramos
Intraspecific Variation In Leaf Traits Across An Environmental Gradient In The Cape Floristic Provence Of South Africa, Bruce H. Ramos
Honors Projects
Intraspecific variability has been an overlooked and under-investigated driving force behind biodiversity. Both interspecific and intraspecific variability contribute to species community assembly, and the South African Cape Floristic Region (CFR), as an area of remarkable biodiversity, provides the perfect opportunity to the latter. The objective of this research is to study intraspecific variability in leaf traits across three biomes that span rainfall and temperature gradients, and to assess the partitioning of that variability across biomes, across shrubs within biomes, and within shrubs of four focal species - Rushia intricata, Aridaria noctiflorum, Diospyros austro-africana and Chrysocoma ciliata. All …
Effects Of Early Experience And Plasticity On Neuronal Morphology Within The Prefrontal Cortex In A Rodent Model Of Hypoxia-Ischemia, Zarah M. Melendez
Effects Of Early Experience And Plasticity On Neuronal Morphology Within The Prefrontal Cortex In A Rodent Model Of Hypoxia-Ischemia, Zarah M. Melendez
Honors Projects
Hypoxia-ischemia (HI) is low oxygenation to the brain paired with low blood supply that can disrupt normal patterns of brain development. HI injury is characterized by many long-term cognitive and behavioral deficits including working memory. Neuronal plasticity due to early sensory or learning experience has been suggested to facilitate recovery of function after neonatal brain injury. Plasticity is the ability for the nervous system, more specifically neurons, and their synapses to modify their function and morphology due to experiences, which in turn correlate with changes in behavior. The objective of the present study was to investigate the effects of neonatal …
Examining Escherichia Coli Plasmid Gene Expression In Vibrio Species B-18, Roberto Ramirez
Examining Escherichia Coli Plasmid Gene Expression In Vibrio Species B-18, Roberto Ramirez
Honors Projects
The goal of this experiment was to determine if lower temperatures could serve to induce runaway replication for the expression of the lacZ gene in Vibrio species B-18 carrying a different runaway replication vector, pJM9487. One experiment compares β-galactosidase activity in cells incubated at 35, 37, and 39°C while the second experiment examines the enzyme levels for cells incubated at either a low temperature or a high temperature in the presence and absence of the chemical inducer used for the promoter (ptrp) that controls the expression of the lacZ gene on this plasmid.
Effects Of Hypoxia-Ischemia And Anti-Inflammatory Prophylactic Treatment On Cortical And Hippocampal Volumes In The Developing Rat Brain, Llian Mabardi
Honors Projects
Very low body weight as a result of premature birth is a common problem all around the world. Many of these infants have medical issues that arise as a direct result of their very low body weight. One of the biggest issues is a lack of oxygen, which is also known as hypoxia. Hypoxia and ischemia (lack of blood flow) are a problem at any age, but they can be especially devastating to infants who have not undergone critical periods of brain development. Hypoxia-ischemia (or HI) can cause problems that start as inflammation and end with large-scale cell death in …
Living In An Age Of Technology: College Students' Perception Of The Role Of Genetic Engineering And Reproductive Technology In Today's Society, Kayla Quadros
Honors Projects
This study examines attitudes about prevalent issues in genetics and reproduction among the college population. Eighteen interviews were conducted with students at Rhode Island College. Respondents were asked questions about their moral standpoints concerning utilizing genetic engineering for disease control and aesthetic purposes, employing assisted reproductive technologies (ARTs), regulation of such procedures, and also about media sources for which they have heard of these topics. Findings suggest that, generally, participants felt that genetic engineering for health issues is permissible, yet reprehensible for the purpose of aesthetically "designing" a baby, though four students (three of which were female) found the enhancement …
Ufd2a Isoforms With Vsp/P97 And Its Ibmpfd Mutants, Amanda St. Germain
Ufd2a Isoforms With Vsp/P97 And Its Ibmpfd Mutants, Amanda St. Germain
Honors Projects
Using the Yeast2Hybrid system, the binding affinities between Ufd2a variants and VCP/p97 mutants were analyzed. Several VCP/p97 mutants are associated with Inclusion body myositis in association of Paget's disease of the bone and fronto temperal dementia (IMBPFD). It was found that Ufd2a bound strongest to the VCP disease causing mutant A232E. It was also determined that the muscle specific isoform of Ufd2a, Ufd2a III, did not interact with VCP/p97.
Species Vs. Height As Predictors Of Increased Growth Rate In New England Canopy Trees, Kiely Schultz
Species Vs. Height As Predictors Of Increased Growth Rate In New England Canopy Trees, Kiely Schultz
Honors Projects
As part of a larger study on the effect of an experimentally-created gap within a forest on adult canopy trees, focuses on the possible effect of tree height versus type of species on radial growth response to gap creation. Sample includes fifty trees within four transects next to a gap in the Yale-Myers Experimental Forest in Connecticut, with results showing that type of species affects radial growth response.
Localization Of Ufd2a Transcripts In Adult And Fry Zebrafish, Serena J. Kankash
Localization Of Ufd2a Transcripts In Adult And Fry Zebrafish, Serena J. Kankash
Honors Projects
Examines the spacio-temporal expression of the three alternative splice forms of UFD2A in zebrafish to further understanding of UFD2A's role in muscle development
Expression Of Three Novel Alternative Splice Forms Of Ufd2a During Zebrafish Development, Nicole Gadbois
Expression Of Three Novel Alternative Splice Forms Of Ufd2a During Zebrafish Development, Nicole Gadbois
Honors Projects
Ufd2a is a ubiquitylation enzyme that is involved in protein degradation, cell division, and apoptosis signaling. Data has shown that cardiac and skeletal muscle cells of adult rodents and humans express alternatively spliced isoforms of Ufd2a. Ufd2a I (exons 1-6, 8-27) has been found to be expressed in murine myoblasts, but, upon differentiation, myotubes began to express Ufd2a II (includes exon 7), followed by Ufd2a III (includes exon 7a). Since zebrafish are an ideal in vivo model organism, this study examines the spatial-temporal expression patterning of Ufd2a and its isoforms in zebrafish embyros during development.
A Tale Of Two Investigations In Molecular Biology: The Use Of Pcr Technology To Identify Bacteria Containing Genes For Pha Synthesis And The Antibiotic Sensitivity Profile Of Vibrio Species B-18, Diana B. Lizarazo
Honors Projects
The Vibrio species, B-18, produces PHA, a bacterial storage material with application as a biodegradable plastic. Phase one of this project aimed at determining if PCR technology could be used to find other bacteria that produce a similar type of PHA. Results showed that the primer sets utilized were not able to identify bacteria with PHA genes. Phase two hoped to discover if it would be possible to introduce Escherichia coli plasmids into B-18 using electroporation. Results demonstrated that electroporation was not useful for introducing plasmids into B-18.