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Use Of Co-Immunoprecipitations And 2d Gel Electrophoresis To Identify Protein-Protein Interactions Of Maturase K, Lauren Angello
Use Of Co-Immunoprecipitations And 2d Gel Electrophoresis To Identify Protein-Protein Interactions Of Maturase K, Lauren Angello
Honors Theses
Maturase K (MatK) is the only group II intron encoded protein in the chloroplast of land plants. Maturases are prokaryotic enzymes that catalyze formation of the lariat structure needed for intron removal from precursor RNAs. The chloroplast maturase MatK, is a descendant of prokaryotic maturases, however, unlike its prokaryotic relatives, MatK is thought to catalyze excision of, not only its own intron, but also the introns of other group II introns in the plastome. Similar to the multiprotein and snRNA spliceosomal complex of the nucleus, it is postulated that MatK is not working alone to excise these introns but most …
A Comparison Of Phytoplankton Nutrient Limitation Between The Marsh And Beach Environments Of Waties Island, Sc, Raymond Sgambati, George Boneillo
A Comparison Of Phytoplankton Nutrient Limitation Between The Marsh And Beach Environments Of Waties Island, Sc, Raymond Sgambati, George Boneillo
Honors Theses
The aim of this study was to investigate the differences in nutrient limitation within the beach and marsh environments at Waties Island, SC. Conducting the experiment on Waties Island allowed most physical factors to be held as constant, meaning the marsh and beach environments would serve as the only variables. Experiments were performed in the winter, spring, summer and fall of 2018 to determine temporal changes in limitation to phytoplankton and cyanobacteria growth. Nutrient additions included dissolved inorganic nitrogen (nitrate, nitrite, and ammonium), dissolved organic nitrogen (urea), and phosphate. To test for co-limitation, a nitrate & phosphate treatment was also …