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The Microbial Community Structure In Petroleum-Contaminated Sediments Corresponds To Geophysical Signatures, Jonathan P. Allen, Estella A. Atekwana, Eliot A. Atekwana, Joseph W. Duris, D. Dale Werkema, Silvia Rossbach
The Microbial Community Structure In Petroleum-Contaminated Sediments Corresponds To Geophysical Signatures, Jonathan P. Allen, Estella A. Atekwana, Eliot A. Atekwana, Joseph W. Duris, D. Dale Werkema, Silvia Rossbach
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
The interdependence between geoelectrical signatures at underground petroleum plumes and the structures of subsurface microbial communities was investigated. For sediments contaminated with light non-aqueous-phase liquids, anomalous high conductivity values have been observed. Vertical changes in the geoelectrical properties of the sediments were concomitant with significant changes in the microbial community structures as determined by the construction and evaluation of 16S rRNA gene libraries. DNA sequencing of clones from four 16S rRNA gene libraries from different depths of a contaminated field site and two libraries from an uncontaminated background site revealed spatial heterogeneity in the microbial community structures. Correspondence analysis showed …
In-Situ Apparent Conductivity Measurements And Microbial Population Distribution At A Hydrocarbon-Contaminated Site, Estella A. Atekwana, D. Dale Werkema, Joseph W. Duris, Silvia Rossbach, Eliot A. Atekwana, William A. Sauck, Daniel P. Cassidy, Jay Means, Franklyn D. Legall
In-Situ Apparent Conductivity Measurements And Microbial Population Distribution At A Hydrocarbon-Contaminated Site, Estella A. Atekwana, D. Dale Werkema, Joseph W. Duris, Silvia Rossbach, Eliot A. Atekwana, William A. Sauck, Daniel P. Cassidy, Jay Means, Franklyn D. Legall
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
We investigated the bulk electrical conductivity and microbial population distribution in sediments at a site contaminated with light nonaqueous-phase liquid (LNAPL). The bulk conductivity was measured using in-situ vertical resistivity probes; the most probable number method was used to characterize the spatial distribution of aerobic heterotrophic and oil-degrading microbial populations. The purpose of this study was to assess if high conductivity observed at aged LNAPL-impacted sites may be related to microbial degradation of LNAPL. The results show higher bulk conductivity coincident with LNAPL-impacted zones, in contrast to geoelectrical models that predict lower conductivity in such zones. The highest bulk conductivity …
Concentrations And Isotope Ratios Of Dissolved Inorganic Carbon In Denitrifying Environments, C. Nascimento, Eliot A. Atekwana, R. V. Krishnamurthy
Concentrations And Isotope Ratios Of Dissolved Inorganic Carbon In Denitrifying Environments, C. Nascimento, Eliot A. Atekwana, R. V. Krishnamurthy
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
We measured the concentration and isotope ratio of dissolved inorganic carbon (DIC) in groundwater associated with denitrification (Corg + NO3- = CO2 + N2) in an agriculturally impacted site in southwestern Michigan. Samples with the lowest nitrate levels also had low dissolved oxygen content and were more depleted in δ13C than background groundwater. All the samples had DIC concentrations in excess of titratable alkalinity. The magnitude of this DIC in excess of alkalinity correlated with a decreasing δ13C attesting to the presence of CO2 derived from organic carbon. Carbon …