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Engineering Thermostable Regulators For Inducible Gene Expression In Thermophiles, Connor M. Joyce
Engineering Thermostable Regulators For Inducible Gene Expression In Thermophiles, Connor M. Joyce
Honors Theses and Capstones
Thermophilic bacteria have attracted research interest due to their ability to grow at high temperatures ranging from 45 °C to 75 °C with some extreme thermophiles able to survive nearly boiling temperatures. They are valuable sources of thermostable biocatalysts and many have great potential as industrial hosts for biofuel production because fermentation at high temperatures has advantages such as reduced processing costs and lower risk of contamination compared with mesophilic bacteria. For example, some Geobacillus species has been shown to have high solvent tolerance, making them good candidates as host for alcohol production. To develop them as industrial hosts, large …
The Pleiotropic Effects Of Beneficial Mutations Of Adapted Escherichia Coli Populations, Brian Scott Van Dam
The Pleiotropic Effects Of Beneficial Mutations Of Adapted Escherichia Coli Populations, Brian Scott Van Dam
Honors Theses and Capstones
Mutations that improve fitness in one environment can often be beneficial, deleterious, or neutral in alternative environments. When a single mutation effects fitness in multiple environments, it is said to be a pleiotropic, which can have important consequences for niche specialization, niche expansion, speciation, and even extinction in the face of environmental change. While previous studies have revealed that pleiotropy is nearly universal, the role of adaptive history in the spectrum of pleiotropic effects has yet to undergo detailed experimental observation. Using experimental evolution we gathered beneficial mutations in a previously adapted strain of Escherichia coli growing in the same …