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A Study Of The Effects Of Consanguinity At The Genomic Level In Two Pakistani Bradaris, Sheena Sullivan
A Study Of The Effects Of Consanguinity At The Genomic Level In Two Pakistani Bradaris, Sheena Sullivan
Theses : Honours
The purpose of the project was to assess the effects of inbreeding on the genetic constitution of two Pakistani bradaris (literally defined as brotherhoods). Both bradaris contain children born to consanguineous (first cousin) and nonconsanguineous marriages. DNA samples have been supplied by Dr Subaib Ahmed of the Armed Forces Institute of Pathology in Rawalpindi, for a total of 91 individuals. The specific allele frequencies, and levels of homozygosity of each bradari, were determined using twenty fluorescence-labelled microsatellite markers for chromosomes 13 and 15. Amplification of the DNA was performed using the polymerase chain reaction (PCR). The PCR products were separated …
Homozygosity Levels In Consanguineous Populations: A Study Of The Sankethi Community, Karnataka, India, Janet Cahill
Homozygosity Levels In Consanguineous Populations: A Study Of The Sankethi Community, Karnataka, India, Janet Cahill
Theses : Honours
The project has studied the inheritance of microsatellite alleles across four chromosomes within two multi-generation families from a highly endogamous community, the Sankethi of Karnataka, India. The two families each have members from four generations participating in the study. Finger prick blood samples and detailed pedigree information were collected from subjects in India. DNA was extracted from the blood spot samples using a phenol-chloroform technique, and analysed by spectrophotometry to determine DNA concentrations. Fluorescent markers from chromosomes 15, 16, 17 & 18 were amplified using polymerase chain reaction (PCR) techniques on a Perkin Elmer 96 well thermocycler. The PCR products …