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- Department of Food Science and Technology: Dissertations, Theses, and Student Research (10)
- Department of Food Science and Technology: Faculty Publications (6)
- Department of Biological Systems Engineering: Dissertations and Theses (2)
- Department of Civil and Environmental Engineering: Faculty Publications (1)
- Honors Theses (1)
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Evaluating Salmonella Cross Contamination In Raw Chicken Thighs In Simulated Post-Chill Tanks, Raziya Sadat
Evaluating Salmonella Cross Contamination In Raw Chicken Thighs In Simulated Post-Chill Tanks, Raziya Sadat
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Salmonella contamination poses a significant risk to food safety, particularly in poultry products and public health. This study aims to investigate the effectiveness of peracetic acid (PAA) treatment in mitigating Salmonella cross-contamination in chicken thighs within simulated post-chill tanks, considering different inoculum concentrations and organic matter (OM) levels.
The experiment involved the use of five distinct Salmonella serovars to inoculate chicken thighs, which were categorized into two groups: higher inoculation level (ca. 7 log CFU/mL) and lower inoculation level (ca, 4 log CFU/mL). These groups underwent treatment with four different solutions, including 540 ppm PAA with 1.5% OM, 540 ppm …
A New Method For Reducing Salmonella In Poultry Meat, Regis Yizerwe
A New Method For Reducing Salmonella In Poultry Meat, Regis Yizerwe
Honors Theses
An important approach to decreasing the transmission of Salmonella into the food chain is to treat raw chicken after processing. This can be, and has been done, by using antibiotics and chemical disinfectants. Using antibiotics is not a viable option due to the issue of antimicrobial resistance. Although some chemical disinfectants have been used effectively, they are restricted due to the amounts that can be used safely, the acceptance of their use and resistance to implementation in various parts of the world. This study has revealed that using a naturally occurring saponin–Quillaja Saponaria– in small concentrations under certain conditions of …
Use Of Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba) To Mitigate Salmonella Enterica, Shiga Toxin-Producing Escherichia Coli (Stec) And Aspergillus Flavus In Pet Food Kibbles, Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Use Of Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba) To Mitigate Salmonella Enterica, Shiga Toxin-Producing Escherichia Coli (Stec) And Aspergillus Flavus In Pet Food Kibbles, Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Department of Food Science and Technology: Faculty Publications
Post-processing operations of extruded pet food kibbles involve coating the product with fats and flavorings. These processes increase the risk for cross-contamination with food-borne pathogens such as Salmonella and Shiga toxin-producing Escherichia coli (STEC), and mycotoxin-producing molds such as Aspergillus spp. after the thermal kill step. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WD-MAX™, against Salmonella enterica, STEC and Aspergillus flavus when used as a coating on pet food kibbles were evaluated. Using canola oil and dry dog digest as fat and flavor …
Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Department of Food Science and Technology: Faculty Publications
Contaminated surfaces can transmit pathogens to food in industrial and domestic food-handling environments. Exposure to pathogens on food contact surfaces may take place via the cross-contamination of pathogens during postprocessing activities. Formaldehyde-based commercial sanitizers in recent years are less commonly being used within food manufacturing facilities due to consumer perception and labeling concerns. There is interest in investigating clean-label, food-safe components for use on food contact surfaces to mitigate contamination from pathogenic bacteria, including Salmonella. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WDMAX …
Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Department of Food Science and Technology: Faculty Publications
Contaminated surfaces can transmit pathogens to food in industrial and domestic food-handling environments. Exposure to pathogens on food contact surfaces may take place via the cross-contamination of pathogens during postprocessing activities. Formaldehyde-based commercial sanitizers in recent years are less commonly being used within food manufacturing facilities due to consumer perception and labeling concerns. There is interest in investigating clean-label, food-safe components for use on food contact surfaces to mitigate contamination from pathogenic bacteria, including Salmonella. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WD-MAX™, …
Antimicrobial Efficacy Of A Citric Acid/Hydrochloric Acid Blend, Peroxyacetic Acid, And Sulfuric Acid Against Salmonella On Inoculated Non-Conventional Raw Chicken Products, Emma Nakimera
Department of Food Science and Technology: Dissertations, Theses, and Student Research
The utilization of non-conventional chicken parts as human food varies widely across countries. The high prevalence of Salmonella, especially in the giblets, poses a high risk to public health. Poultry processors have implemented multiple hurdle technology to reduce this gram-negative pathogen in chicken parts. This study was conducted to evaluate the antimicrobial efficacy of a commercial blend of citric acid/ hydrochloric acid (CP), peroxyacetic acid (PAA), and sulfuric acid (SA) in reducing Salmonella inoculated on two chicken giblets: livers and hearts. Chicken hearts and livers were inoculated (6 log CFU/ml of rinsate) by individually immersing them in a cocktail …
Inactivation Of Salmonella In Black Peppercorn By Fluidization With Hydrogen Peroxide Vapor, Edel Summers
Inactivation Of Salmonella In Black Peppercorn By Fluidization With Hydrogen Peroxide Vapor, Edel Summers
Department of Biological Systems Engineering: Dissertations and Theses
The purpose of the present study was to assess the effectiveness of hydrogen peroxide vapor (HPV) at inactivating Salmonella inoculated in whole black pepper when used as fluidizing gas media. HPV treatment was conducted at two different temperatures (45°C and 60°C) and two dwell times (30 min and 60 min). Microbial reduction and residual hydrogen peroxide were measured at three storage times: 0 h (immediately after treatment), 24 h and 48 h post-treatment. The effect of HPV on the quality of whole black peppercorn was evaluated 48 h post-treatment based on changes in piperine content, total phenolics, antioxidant activity, total …
Evaluating The Microbial Quality And Use Of Antimicrobials In Raw Pet Foods, Leslie Pearl Cancio
Evaluating The Microbial Quality And Use Of Antimicrobials In Raw Pet Foods, Leslie Pearl Cancio
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Raw meat-based diets (RMBDs) are emerging pet foods that pose food safety risks because of the potential presence of pathogens that could cause illness to humans. In this research, the microbial quality of select RMBD products sold by pet food companies online and the use of chemical antimicrobials to reduce the microbial load in chicken liver, a common RMBD ingredient, were evaluated.
Ground meat blends and livers from four animal species (beef, pork, chicken, turkey) were purchased from four online companies that delivers directly to consumers through parcel businesses. Products were procured at three different times during one year and …
Thermal Inactivation Of Salmonella On Chicken Wings Cooked In Domestic Convection And Air Fryer Ovens, Carmen Cano, Xinyao Wei, Cyril A. Etaka, Byron D. Chaves
Thermal Inactivation Of Salmonella On Chicken Wings Cooked In Domestic Convection And Air Fryer Ovens, Carmen Cano, Xinyao Wei, Cyril A. Etaka, Byron D. Chaves
Department of Food Science and Technology: Faculty Publications
Chicken wings are among the most popular poultry products for home and foodservice consumption. Poultry products must be handled and cooked safely to decrease the risk of foodborne salmonellosis for consumers. This study aims to validate the use of domestic appliances (convection and air fryer ovens) for the thermal inactivation of Salmonella on chicken wings. Wings (n = 3, 46.5 ± 4.3 g) were inoculated with a five-strain cocktail of Salmonella (ca. 8 log10 CFU/wing) and cooked in a convection oven (179.4ºC) or an air fryer (176, 190, or 204ºC) for 2, 5, 10, 15, 20, 22, or 25 …
Microbial Challenge Studies Of Radio Frequency Heating For Dairy Powders And Gaseous Technologies For Spices, Xinyao Wei
Microbial Challenge Studies Of Radio Frequency Heating For Dairy Powders And Gaseous Technologies For Spices, Xinyao Wei
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Persistence, thermal resistance, and survival of Salmonella in low moisture foods (LMF) have resulted in several foodborne illness outbreaks. Both existing and novel pasteurization technologies need to be validated for microbial safety of LMF. In this dissertation, a framework for using radio frequency (RF) heating to enhance microbial safety of milk powders and egg white powder was established. Thermal inactivation kinetics of Salmonella in milk powders were determined to guide the dairy industry for identifying thermal processing conditions for pasteurization. Storage time showed no effect on the thermal resistance of Salmonella, which can simplify the process validation study in …
Process Interventions For Improving The Microbiological Safety Of Low Moisture Food Ingredients, Tushar Verma
Process Interventions For Improving The Microbiological Safety Of Low Moisture Food Ingredients, Tushar Verma
Department of Food Science and Technology: Dissertations, Theses, and Student Research
The recurrence of Salmonella in low moisture foods and the implementation of the FSMA rule requires a need to validate legacy and novel processing technologies. In this dissertation, a legacy thermal (extrusion), a novel thermal (radiofrequency (RF) heating), and a non-thermal (chlorine dioxide) technology, were evaluated as intervention technologies for Salmonella in low moisture foods. The twin-screw extruder was performed at different levels of screw speeds, temperatures, moisture contents, and fat contents to understand the impact of processing conditions on Salmonella inactivation in oat flour. At temperature >65°C, the Salmonella population was below the detection limit. At 55°C, Salmonella reduction …
Improving Microbiological Safety Of Low Moisture Food Products Using Radio Frequency And Ethylene Oxide, Long Chen
Department of Biological Systems Engineering: Dissertations and Theses
Recent foodborne illness outbreaks in US associated with consumption of low-moisture foods (LMF) have heightened concerns of their microbial safety. Salmonella is a pathogen of major concern in LMF due to its ability to persist in low water activity (aw) environments. The disadvantages of existing decontamination technologies for LMF call for novel and efficient intervention technologies. Radio frequency (RF) and ethylene oxide (EtO) were evaluated in this dissertation for improving microbial food safety and quality of LMF. Cumin seeds and inshell hazelnuts were selected as model foods.
It took < 2 min of stationary RF heating to achieve > 5 log reductions of Salmonella in cumin seeds without …
Validation Of Extrusion Processing For The Safety Of Low-Moisture Foods, Tushar Verma
Validation Of Extrusion Processing For The Safety Of Low-Moisture Foods, Tushar Verma
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Salmonella in low-moisture foods is an emerging challenge due to numerous food product recalls and foodborne illness outbreaks. The new Food Safety Modernization Act requires the food processors to validate their process controls that indeed kills the desired number of bacteria. This research had two major objectives: 1) Develop a response surface model for Salmonella inactivation during the extrusion of low-moisture food 2) Evaluate the use of Enterococcus faecium NRRL B-2354 as an adequate surrogate for Salmonella during the extrusion of low-moisture food. Oat flour was selected as a low-moisture food model. For inoculation, cocktail of five different strains of …
Simulation And Validation Of Radio Frequency Heating Of Shell Eggs, Soon Kiat Lau
Simulation And Validation Of Radio Frequency Heating Of Shell Eggs, Soon Kiat Lau
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Finite element models were developed with the purpose of finding an optimal radio frequency (RF) heating setup for pasteurizing shell eggs. Material properties of the yolk, albumen, and shell were measured and fitted into equations that were used as inputs for the model. When the egg was heated by itself, heating tend to be focused at the air cell to result in a “coagulation ring.” The focused heating near the air cell of the egg prevented satisfactory pasteurization of the egg, but deeper analysis of the simulation results offered a new perspective on how non-uniform RF heating could occur in …
Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt
Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt
Department of Civil and Environmental Engineering: Faculty Publications
The use of wastewater for irrigation may introduce antimicrobials and human pathogens into the food supply through vegetative uptake. The objective of this study was to investigate the uptake of three antimicrobials and Salmonella in two lettuce cultivars. After repeated subirrigation with synthetic wastewater, lettuce leaves and soil were collected at three sequential harvests. The internalization frequency of Salmonella in lettuce was low. A soil horizon-influenced Salmonella concentration gradient was determined with concentrations in bottom soil 2 log CFU/g higher than in top soil. Lincomycin and sulfamethoxazole were recovered from lettuce leaves at concentrations as high as 822 ng/g and …
Salmonella Enterica Serovar Typhimurium-Infected Pigs With Different Shedding Levels Exhibit Distinct Clinical, Peripheral Cytokine And Transcriptomic Immune Response Phenotypes, Susan Knetter, Shawn Bearson, Ting-Hua Huang, Dason Kurkiewicz, Martine Schroyen, Dan Nettleton, Daniel Berman, Valorie Cohen, Joan Lunney, Amanda Ramer-Tait, Michael Wannemuehler, Christopher Tuggle
Salmonella Enterica Serovar Typhimurium-Infected Pigs With Different Shedding Levels Exhibit Distinct Clinical, Peripheral Cytokine And Transcriptomic Immune Response Phenotypes, Susan Knetter, Shawn Bearson, Ting-Hua Huang, Dason Kurkiewicz, Martine Schroyen, Dan Nettleton, Daniel Berman, Valorie Cohen, Joan Lunney, Amanda Ramer-Tait, Michael Wannemuehler, Christopher Tuggle
Department of Food Science and Technology: Faculty Publications
Foodborne salmonellosis costs the US $2.7 billion/year, including $100.0 million in annual losses to pork producers. Pigs colonized with Salmonella are usually asymptomatic with varied severity and duration of fecal shedding. Thus, understanding the responses that result in less shedding may provide a mechanism for control. Fifty-four pigs were inoculated with Salmonella enterica serovar Typhimurium (ST) and clinical signs, fecal ST shedding, growth performance, peripheral cytokines and whole blood gene expression were measured. Persistently shedding (PS) pigs had longer pyrexia and elevated serum IL-1β, TNF-α and IFN-ϒcompared with low shedding (LS) pigs, while LS pigs had brief pyrexia, less shedding …
Analyzing Cellular Internalization Of Nanoparticles And Bacteria By Multi-Spectral Imaging Flow Cytometry, Yashdeep Phanse, Amanda E. Ramer-Tait, Sherree L. Friend, Brenda Carrillo-Conde, Paul Lueth, Carrie J. Oster, Gregory J. Phillips, Balaji Narasimhan, Michael J. Wannemuehler, Bryan H. Bellaire
Analyzing Cellular Internalization Of Nanoparticles And Bacteria By Multi-Spectral Imaging Flow Cytometry, Yashdeep Phanse, Amanda E. Ramer-Tait, Sherree L. Friend, Brenda Carrillo-Conde, Paul Lueth, Carrie J. Oster, Gregory J. Phillips, Balaji Narasimhan, Michael J. Wannemuehler, Bryan H. Bellaire
Department of Food Science and Technology: Faculty Publications
Nanoparticulate systems have emerged as valuable tools in vaccine delivery through their ability to efficiently deliver cargo, including proteins, to antigen presenting cells1-5. Internalization of nanoparticles (NP) by antigen presenting cells is a critical step in generating an effective immune response to the encapsulated antigen. To determine how changes in nanoparticle formulation impact function, we sought to develop a high throughput, quantitative experimental protocol that was compatible with detecting internalized nanoparticles as well as bacteria. To date, two independent techniques, microscopy and flow cytometry, have been the methods used to study the phagocytosis of nanoparticles. The high throughput …
Identification And Characterization Of Salmonella Serotypes Isolated From Pork And Poultry From Commercial Sources, Yulie Edith Meneses-GonzáLez
Identification And Characterization Of Salmonella Serotypes Isolated From Pork And Poultry From Commercial Sources, Yulie Edith Meneses-GonzáLez
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Salmonella is one of the leading causes of foodborne illness worldwide, and it is estimated that 1.4 million infections occur annually in the U.S. alone. The Premi®Test Salmonella (PTS), is a potential tool for rapid detection and identification of Salmonella serovars. The objective of this project was to evaluate the use of the PTS system as a serotyping tool to identify pork and poultry isolates obtained from vertically integrated operations and to characterize their antibiotic resistance. Two hundred isolates were evaluated. All isolates were serotyped using the traditional Kaufmann-White scheme and the PTS system. Among the isolates 63 different serotypes …
The Effects Of High Pressure Processing On Peanut Sauce Inoculated With Salmonella, Tara K. Stiles
The Effects Of High Pressure Processing On Peanut Sauce Inoculated With Salmonella, Tara K. Stiles
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Recent Salmonella outbreaks have prompted the need for new processing options for peanut products. Traditional heating kill-steps have shown to be ineffective in lipid-rich matrices such as peanut products. High pressure processing is one such option for peanut sauce because it has a high water activity, which has proved to be a large contributing factor in microbial lethality due to high pressure processing. Four different formulations of peanut sauce were inoculated with a five strain Salmonella cocktail and high pressure processed. Results indicate that increasing pressure or increasing hold time increases log10 reductions. The Weibull model was fitted to …
Dynamic Predictive Model For The Growth Of Salmonella Spp. In Liquid Whole Egg And Risk Evaluation Of Egg White Hydrolysates Manufacturing Process For Spore Formers: Bacillus Cereus And Clostridium Perfringens., Aikansh Singh
Department of Food Science and Technology: Dissertations, Theses, and Student Research
A dynamic model for the growth of Salmonella spp. in liquid whole egg under continuously varying temperature was developed and validated. A primary model (Baranyi model) was fitted to each temperature growth data and corresponding maximum growth rates were estimated. Pseudo-R2 values were greater than 0.97 for primary models. The maximum growth rates obtained from each primary model were then plotted against temperature and modeled using the modified Ratkowsky model. The pseudo-R2 and root mean square error were 0.99 and 0.06 h-1, respectively for the secondary model. A dynamic model for the prediction of Salmonella spp. …