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Ph Homeostasis In Lactic Acid Bacteria, Robert W. Hutkins, Nancy L. Nannen
Ph Homeostasis In Lactic Acid Bacteria, Robert W. Hutkins, Nancy L. Nannen
Department of Food Science and Technology: Faculty Publications
The ability of lactic acid bacteria to regulate their cytoplasmic or intracellular pH is one of the most important physiological requirements of the cells. Cells unable to maintain a near neutral intracellular pH during growth or storage at low extracellular pH may lose viability and cellular activity. Despite the importance of pH homeostasis in the lactic acid bacteria, however, an understanding of cytoplasmic pH regulation has only recently begun to emerge. This review describes the specific effects of low pH on lactic acid bacteria, reports recent research on the physiological role of intracellular pH as a regulator of various metabolic …
United States Patent: Composition And Method For Inhibiting Pathogens And Spoilage Organisms In Food, Robert W. Hutkins, Elaine D. Berry, Michael B. Liewen
United States Patent: Composition And Method For Inhibiting Pathogens And Spoilage Organisms In Food, Robert W. Hutkins, Elaine D. Berry, Michael B. Liewen
Department of Food Science and Technology: Faculty Publications
The method of the invention uses live cells of non-fermenting and/or non-growing lactic acid bacteria to deliver bacteriocin into edible food substances to inhibit the growth of food spoilage and/or food-borne pathogenic organisms. The method of the invention may be used to inhibit growth of these organisms in raw food substances and finished food products after processing. The lactic acid bacteria within the food mixture are capable of producing bacteriocin in the desired microbial- inhibiting amounts under conditions of non-growth and non-fermentation
Regulation Of ΣB Levels And Activity In Bacillus Subtilis, Andrew K. Benson, William G. Haldenwang
Regulation Of ΣB Levels And Activity In Bacillus Subtilis, Andrew K. Benson, William G. Haldenwang
Department of Food Science and Technology: Faculty Publications
The sigB operon of Bacilus subtilis encodes (σB plus three additional proteins (RsbV, RsbW, and RsbX) that regulate σB activity. Using an anti-σB monoclonal antibody to monitor the levels of (σB protein, PSPAC to control the expression of the sigB operon, and a ctc-lacZ reporter system to monitor σB activity, we observed that the rsbV and rsbW products control (σB activity at the ctc promoter independently of their effects on (σB levels. In contrast, RsbX was found to have no effect on expression of ctc when the sigB operon was controlled by …
Bacillus Subtilis ΣB Is Regulated By A Binding Protein (Rsbw) That Blocks Its Association With Core Rna Polymerase, Andrew K. Benson, William G. Haldenwang
Bacillus Subtilis ΣB Is Regulated By A Binding Protein (Rsbw) That Blocks Its Association With Core Rna Polymerase, Andrew K. Benson, William G. Haldenwang
Department of Food Science and Technology: Faculty Publications
σB is a secondary σ factor of Bacillus subtilis. RNA polymerase containing cr transcribes a subset of genes that are expressed after heat shock or the onset of the stationary phase of growth. Three genes (rsbV, rsbW, and rsbX), cotranscribed with the σB structural gene (sigB), regulate o dependent gene expression. RsbW is the primary inhibitor of this system with the other gene products acting upstream of RsbW in the σB regulatory pathway. Evidence is now presented that RsbW inhibits σB-dependent transcription by binding to crB and blocking …
The ΣB-Dependent Promoter Of The Bacillus Subtilis Sigb Operon Is Induced By Heat Shock, Andrew K. Benson, W. G. Haldenwang
The ΣB-Dependent Promoter Of The Bacillus Subtilis Sigb Operon Is Induced By Heat Shock, Andrew K. Benson, W. G. Haldenwang
Department of Food Science and Technology: Faculty Publications
σB, a secondary sigma factor of Bacillus subtilis, was found to increase 5- to 10-fold when cultures were shifted from 37 to 48°C. Western blot (immunoblot) analyses, in which monoclonal antibodies specific for the sigB operon products RsbV, RsbW, and σB were used to probe extracts from wild-type and mutant B. subtilis strains, revealed that all three proteins increased coordinately after heat shock and that this increase was dependent on σB but not RsbV, a positive regulator normally essential for σB-dependent sigB expression. Nuclease protection experiments of RNA synthesized after heat shock supported …