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Significance Of Hemolytic Activity Of Some Radiation- Resistant Micrococci In Food, Ardyce Welch, R. Burt Maxcy
Significance Of Hemolytic Activity Of Some Radiation- Resistant Micrococci In Food, Ardyce Welch, R. Burt Maxcy
Department of Food Science and Technology: Faculty Publications
Micrococci resistant to 1 Mrad ofgamma radiation were isolated from irradiated chicken. Three isolates were hemolytic on blood agar plates and were selected for further study.Two other radiation-resistant micrococci, Micrococcus radiodurans and Micrococcus radiophilus, were included in the study because there is only a very limited amount of information regarding hemolytic activity of these organisms and their potential role of public health importance. Tests to determine hemolytic patterns, hemolytic activity of extracellular substances, leukocytic activity, presence of enzymes commonly associated with pathogenicity (coagulase, deoxyribonuclease, phosphatase), and pathogenicity for laboratory animals all suggested that the organisms would not be of …
Isolation Of Radiation-Resistant Bacteria Without Exposure To Irradiation, Scott Sanders, R. Burt Maxcy
Isolation Of Radiation-Resistant Bacteria Without Exposure To Irradiation, Scott Sanders, R. Burt Maxcy
Department of Food Science and Technology: Faculty Publications
Resistance to desiccation was utilized in the selection of highly radiation-resistant asporogenous bacteria from non-irradiated sources. A bacterial suspension in phosphate buffer was dried in a thin film at 25°C and 33% relative humidity. Storage under these conditions for 15 days or more reduced the number of radiation-sensitive bacteria. Further selection for radiation-resistant bacteria was obtained by irradiation of bacteria on velveteen in the replication process, thereby avoiding the toxic effect of irradiated media. The similarity of radiation resistance and identifying characteristics in irradiated and non-irradiated isolates should allay some concerns that highly radiation-resistant bacteria have been permanently altered by …
Patterns Of Cell Division, Dna Base Compositions, And Fine Structures Of Some Radiation-Resistant Vegetative Bacteria Found In Food, S. W. Sanders, R. Burt Maxcy
Patterns Of Cell Division, Dna Base Compositions, And Fine Structures Of Some Radiation-Resistant Vegetative Bacteria Found In Food, S. W. Sanders, R. Burt Maxcy
Department of Food Science and Technology: Faculty Publications
Representative highly radiation-resistant Moraxella-Acinetobacter (M-A), Pseudomonas radiora, Micrococcus radiodurans, and Micrococcus radiophilus exhibited a wide variety of division systems and cell wall characteristics. However, the most resistant M-A possessed unusually thick cell walls, indicating a possible role of the cell wall in radiation resistance in the M-A. Thick septation was present in most of the bacteria studied, but was absent in P. radiora, thus excluding this as a necessity for high resistance. Reliable determination of the number of division planes of the M-A for use as a taxonomic criterion was achieved by the direct observation of dividing cells. …
Quantitation Of Proteinaceous Soil Removal From Clean-In-Place Systems, L. M. Rasmussen, R. B. Maxcy
Quantitation Of Proteinaceous Soil Removal From Clean-In-Place Systems, L. M. Rasmussen, R. B. Maxcy
Department of Food Science and Technology: Faculty Publications
The protein of milk residue can be quantitated to evaluate a cleaning process by monitoring the accumulation of protein in a test-wash solution. The test for protein is based on precipitation of hard water cations with orthophosphate and flocculation with a coagulant for rapid sedimentation of the protein. The sediment is collected on filter paper and stained with xylene brilliant cyanin G. Color reaction on the filter paper is proportional to protein content of the original test solution and is estimated visually. For more precise quantitation, the stain is released into a solution for optical measurements. The lower limit of …