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Simultaneous Determination Of 6 Flavonoids In Health Care Products By High Performance Liquid Chromatography, Wu Yi-Qi, Liang Zhi-Sen, Chen Yu-Zhen, Zhou Rui, Lin Xiao-Jun
Simultaneous Determination Of 6 Flavonoids In Health Care Products By High Performance Liquid Chromatography, Wu Yi-Qi, Liang Zhi-Sen, Chen Yu-Zhen, Zhou Rui, Lin Xiao-Jun
Food and Machinery
Objective: To establish a method for simultaneous determination of wogonoside, 4'-hydroxywogonin, norwogonin, baicalein, wogonin and chrysin in health care products by high performance liquid chromatography (HPLC). Methods: The samples were extracted with acetone, C18 solid phase extraction column was used for purification. The target compounds were determined by HPLC, quantified by matrix-matched standard curve method. Phenomenex Luna®C18(250 mm×4.6 mm, 5 μm)column was applied, mobile phase was acctonitrile-acetic acid, using gradient elution)at the flow rate of 1.0 mL/min. The temperature of column was 30 ℃ and the detection wavelength was set at 280 nm. …
Determination Of Four Kinds Of Illegally-Added Artificial Sweeteners In Syrups By High Performance Liquid Chromatography, Zhong Fei-Fei, Lei De-Qing, Zhou Jin-Sha, Liu Bo, Wang Zi-Qing
Determination Of Four Kinds Of Illegally-Added Artificial Sweeteners In Syrups By High Performance Liquid Chromatography, Zhong Fei-Fei, Lei De-Qing, Zhou Jin-Sha, Liu Bo, Wang Zi-Qing
Food and Machinery
Objective: A high performance liquid chromatography method was established for determination of four artificial sweeteners in syrups, i.e., acesulfame, saccharin sodium, aspartame and advantame.Methods:Acetonitrile was used as mobile phase A, and 0.02 mol/L ammonium dihydrogen phosphate (pH value adjusted to 5.5 by triethylamine) was used as mobile phase B. The gradient elution was conducted, with flow rate at 1.0 mL/min,and the column temperature was at 35 ℃; the detection wavelength was 214 nm, and sample size was 10 μL was loaded on the was Shiseido AQ-C18 column (4.6 mm×250 mm, 5 μm) A total of 45 …
Evaluation On Uncertainty Of Advantame In Syrups Determined By High Performance Liquid Chromatography, Lei De-Qing, Zhong Fei-Fei, Zhou Jin-Sha, Liu Bo, Wang Zi-Qian, Zhou Ya-Min
Evaluation On Uncertainty Of Advantame In Syrups Determined By High Performance Liquid Chromatography, Lei De-Qing, Zhong Fei-Fei, Zhou Jin-Sha, Liu Bo, Wang Zi-Qian, Zhou Ya-Min
Food and Machinery
Objective: This study aimed to evaluate the uncertainty of determination by high performance liquid chromatography (HPLC) for the content of adevantame, a synthetic sweetener illegally added in syrup drugs. Methods: The sample is diluted with solvent, and the content of adevantame in the sample was determined by HPLC. Through the analysis of the factors affecting the uncertainty, such as the preparation of the reference solution, curve fitting, sample weighing, sample volumetric determination and instrument precision, the uncertainty was synthesized of each component, and the extended uncertainty is calculated. Results: The expanded uncertainty is 1.8 μg/mL. The influence …
Study On Extraction Process Of Methylated Catechin From Zijuan Tea By Response Surface Methodology, Li Yu-Qian, Gao Chuang-Chuang, Li Ming-Chao, Li Xiao-Lei, Hao Qian
Study On Extraction Process Of Methylated Catechin From Zijuan Tea By Response Surface Methodology, Li Yu-Qian, Gao Chuang-Chuang, Li Ming-Chao, Li Xiao-Lei, Hao Qian
Food and Machinery
Objective:optimizing the extraction process of methylated catechin from Zijuan tea.Methods:Using acid ethanol as extraction solvent, based on three single factor experiments, the extraction time, extraction temperature and liquid-solid ratio were used to investigate the interaction of the above three factors on the extraction rate of methylated catechin from Zijuan tea.Results:The optimum extraction conditions were as follows: extraction time 100 min, extraction temperature 75 ℃, solid-liquid ratio(m Zijuan tea∶VSolvent)1∶74(g/mL); under these conditions, the mass concentration of methylated catechin in the extract could reach 26.7 mg/g, and the relative error with the verification value was 1.48%.Conclusion:HPLC and …
Uncertainty Evaluation Of The Determination Of Vitamin C In Fruits And Vegetables By High Performance Liquid Chromatography, Wang Xia, Zhang Wei-Yi, Han Yi-Yi, Chen Mei-Lian, Feng Dong-Sheng
Uncertainty Evaluation Of The Determination Of Vitamin C In Fruits And Vegetables By High Performance Liquid Chromatography, Wang Xia, Zhang Wei-Yi, Han Yi-Yi, Chen Mei-Lian, Feng Dong-Sheng
Food and Machinery
To evaluate the uncertainty of the determination of vitamin C in fruits and vegetables by high performance liquid chromatography, a mathematical model was established to evaluate each uncertainty component, and the standard uncertainty and extended uncertainty of the synthesis were calculated. The results showed that the amounts of vitamin C in samples was 33.7 mg/100 g, and the expanded uncertainty was 1.4 mg/100 g (k=2). The uncertainties of the determination mainly come from the preparation of standard solution and the repeatability of determination.
Screening And Identification Of Biogenic Amine Degrading Strains, Du Xue, You Yan-Feng
Screening And Identification Of Biogenic Amine Degrading Strains, Du Xue, You Yan-Feng
Food and Machinery
Strains with biodegradability of biogenic amines were screened from lactic acid bacteria strains from different sources preserved in the laboratory, and their growth ability at different salt concentrations and pH values were determined. The results showed that three strains with the capability of degrading biogenic amines were screened out. Based on 16S rDNA sequence analysis, strain HM22 was identified as Lactobacillus fermentum, strain HM24 as Lactobacillus plantarum, and strain YF10042 as Enterococcus faecalis. Among them, Lactobacillus plantarum HM24 has the best degradation ability. The degradation rates of tryptamine, phenethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine are …
Study On The Preparation Of Tetracyline Magnetic Molecularly Imprinted Biochar Microspheres Based On Pickering Emulsion Polymerization, Ma Zhen-Zhen, He Jin-Xing, Zhao Tao, Zhao Xiao-Lei
Study On The Preparation Of Tetracyline Magnetic Molecularly Imprinted Biochar Microspheres Based On Pickering Emulsion Polymerization, Ma Zhen-Zhen, He Jin-Xing, Zhao Tao, Zhao Xiao-Lei
Food and Machinery
In this study, magnetic molecularly imprinted biochar microspheres for specific adsorption of tetracylines (TCs) were prepared by Pickering emulsion polymerization using tetracyline (TC) as template and Fe3O4 and biochar as co-stabilizers. The adsorption properties of the imprinted microspheres for TC were studied by adsorption kinetics test, adsorption isothermal test, and selectivity adsorption test, exhibiting the excellent specific adsorption for TC with imprinting factor of 3.97. Further more, the imprinted materials were used as adsorbents for extraction and purification of TCs in real samples, and the content of TCs were detected through high performance liquid chromatography (HPLC). Under …
Rapid Detection Of Alternaria Monomethyl Ether In Wheat By High Performance Liquid Chromatography, Xu Qun-Ying, Zhai Yu-Heng
Rapid Detection Of Alternaria Monomethyl Ether In Wheat By High Performance Liquid Chromatography, Xu Qun-Ying, Zhai Yu-Heng
Food and Machinery
In this study, a high-performance liquid chromatography (HPLC) method for the efficient detection of alternariolmethyl ether (AME) in wheat was developed. The sample was extracted with acetonitrile/ methanol/ water (volume ratio 45/10/45), shaken at 30 ℃ for 30 minutes, and purified by Bond Elut Plexa solid-phase extraction cartridge. Then the sample was detected with a Variable wavelength detector. In this method, the limit of detection (LOD) of AME was 2.52 μg/kg, and the limit of quantification (LOQ) was 8.40 μg/kg, and the linear range was 0.25~2.00 μg/mL. The concentration of AME recovery was 80.1%~88.3%, the intra-day precision and inter-day precision …
Establish Ment Of A High-Speed Method With Simultaneous Determination Of Multi-Component Sweeteners And Preservatives In Food, Cheng Shui-Lian, He Jian-Guo, Lu Gui-Ying, Yan Xu-Chao, Fu Bai-Ting
Establish Ment Of A High-Speed Method With Simultaneous Determination Of Multi-Component Sweeteners And Preservatives In Food, Cheng Shui-Lian, He Jian-Guo, Lu Gui-Ying, Yan Xu-Chao, Fu Bai-Ting
Food and Machinery
A qualitative and quantitative analysis method has been established forsimultaneous determination of six kinds of sweeteners (sodium cyclamate, saccharinsodium, acesulfame K, trichlorosucrose, aspartame and neotame) and five kinds of preservatives (natamycin, sodium benzoate, potassium sorbate, sodium dehydroacetate and sodium diacetate) in food by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The sample did not need to derive and purify, directing ultrasound extraction with methanol and water, separated on a Shim-pack-ODS Ⅲ column (75 L ×2.0 mm, 1.6 μm), used acetic acid ammonium (10 mmol/L)-methanol as the mobile phase for gradient elution. The ionization efficiencies of target compounds in mass spectrometry were analyzed …