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Investigation Of Rice Bran Derived Anti-Cancer Pentapeptide For Mechanistic Potency In Breast Cancer Cell Models, Ruiqi Li

Graduate Theses and Dissertations

Bioactive peptides derived from food sources with anti-proliferative properties against cancer have drawn more attention in recent years. A pentapeptide derived from rice bran has shown anti-proliferative propertiesagainst human breast cancer cells. The objective of this study was to investigate the mechanistic action of the pentapeptide-induced apoptosis in breast cancer cell models (MCF-7 and MDA-MB-231). The growth inhibition activity of the pentapeptide was

evaluated by MTS[3-(4,5-dimethylthiazol-2-yl)-5-(3- arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] assayand trypan blue assay in a dose- and time-dependent manner.

The apoptotic properties of pentapeptide-induced apoptosis on cancerous breast cells were evaluated by morphological changes, DNA fragmentation, and caspases-3/7, -8,and -9 …

Stability Of Nano-Encapsulated Rice Bran Derived Bioactive Pentapeptide In Apple Juice, Fatima Mohammed Alessa

Graduate Theses and Dissertations

Cereal grains and their components derived Bioactive compounds such as rice bran can promote health and can be derived from Rice bran contains 12-20 % protein and could be a good source for extracting bioactive peptides. A pentapeptide with a secqunce of amino acids Glu-Gln-Arg-Pro-Arg (EQRPR) has been prepared from heat stablized defatted rice bran (HDRB) and has demonstrated anti-cancer proprerties in-vitro. This bioactive pentapeptide can thus be used as a nutraceutical by incorporating it into a suitable food system. Fruit juces can be vehicles to incorporate this pentapeptide. Fruit juices contribute to about 60% of the consumed beverages in …