Digital Commons Network^™

First Proof Of Concept Of Sustainable Metabolite Production From High Solids Fermentation Of Lignocellulosic Biomass Using A Bacterial Co-Culture And Cycling Flush System, Wanying Yao, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

To improve the lignocellulose conversion for ABE in high solids fermentation, this study explored the feasibility of cycling the process through the cellulolytic or/and solventogenic phases via intermittent flushing of the fermentation media. Five different flushing strategies (varying medium ingredients, inoculum supplement and cycling through phases) were investigated. Flushing regularly throughout the cellulolytic phase is necessary because re-incubation at 65 °C significantly improved glucose availability by at least 6-fold. The solvents accumulation was increased by 4-fold using corn stover (3-fold using miscanthus) over that produced by flushing only through the solventogenic phase. In addition, cycling process was simplified by re-incubating …

Catechol Oxidation By Ozone And Hydroxyl Radicals At The Air-Water Interface, Elizabeth A. Pillar, Robert C. Camm, Marcelo I. Guzman

Chemistry Faculty Publications

Anthropogenic emissions of aromatic hydrocarbons promptly react with hydroxyl radicals undergoing oxidation to form phenols and polyphenols (e.g., catechol) typically identified in the complex mixture of humic-like substances (HULIS). Because further processing of polyphenols in secondary organic aerosols (SOA) can continue mediated by a mechanism of ozonolysis at interfaces, a better understanding about how these reactions proceed at the air–water interface is needed. This work shows how catechol, a molecular probe of the oxygenated aromatic hydrocarbons present in SOA, can contribute interfacial reactive species that enhance the production of HULIS under atmospheric conditions. Reactive semiquinone radicals are quickly produced upon …

Vegetation Controls On Weathering Intensity During The Last Deglacial Transition In Southeast Africa, Sarah J. Ivory, Michael M. Mcglue, Geoffrey S. Ellis, Anne-Marie Lézine, Andrew S. Cohen, Annie Vincens

Earth and Environmental Sciences Faculty Publications

Tropical climate is rapidly changing, but the effects of these changes on the geosphere are unknown, despite a likelihood of climatically-induced changes on weathering and erosion. The lack of long, continuous paleo-records prevents an examination of terrestrial responses to climate change with sufficient detail to answer questions about how systems behaved in the past and may alter in the future. We use high-resolution records of pollen, clay mineralogy, and particle size from a drill core from Lake Malawi, southeast Africa, to examine atmosphere-biosphere-geosphere interactions during the last deglaciation (~ 18-9 ka), a period of dramatic temperature and hydrologic changes. The …

Interactive Comment On “Iodine Oxide In The Global Marine Boundary Layer” By C. Prados-Roman Et Al., Marcelo I. Guzman

Chemistry Faculty Publications

No abstract provided.

Northeast‐Oriented Transpression Structure In The Northern New Madrid Seismic Zone: Extension Of A Shear Zone Across The Reelfoot Fault Stepover Arm, Edward W. Woolery, Ali Almayahi

Earth and Environmental Sciences Faculty Publications

High‐resolution seismic‐reflection profiles recently acquired 12 km northeast of the New Madrid seismic zone’s Reelfoot thrust and along the central axis of the Reelfoot rift, imaged steeply dipping N30°E striking faults that have uplifted and arched post‐Paleozoic sediments in a manner consistent with a dextral strike‐slip component of displacement. The subparallel fault strands have been traced 1.4 km between reflection profiles. In order to evaluate the structure’s potential regional scale, the strike was projected northeast 22 km to its intersection with a nearby industry profile. At the intersection, this lower‐resolution profile exhibits a discrete 0.75 km wide structure with style …

The MW 4.2 Perry County, Kentucky, Earthquake Of 10 November 2012: Evidence Of The Eastern Tennessee Seismic Zone In Southeastern Kentucky, N. Seth Carpenter, Edward W. Woolery, Zhenming Wang

Earth and Environmental Sciences Faculty Publications

The 10 November 2012 M_w 4.2 Perry County earthquake may represent a continuation of the seismically active Eastern Tennessee seismic zone (ETSZ) farther north than previously recognized into southeastern Kentucky. The mainshock and aftershock data from regional seismic networks and EarthScope’s Transportable Array stations allowed high‐quality determinations of the source parameters. The focal mechanism, depth, and proximity of the mainshock to the New York–Alabama magnetic lineament, a subsurface, crustal‐scale structure that spatially correlates with central ETSZ seismicity, suggest that this earthquake may share the same type of causal geologic structures as the more‐active ETSZ region to the south.

Co2 Recycling Using Microalgae For The Production Of Fuels, Michael H. Wilson, John Groppo, Andrew Placido, S. Graham, S. A. Morton Iii, Eduardo Santillan-Jimenez, Aubrey Shea, Mark Crocker, Czarena Crofcheck, Rodney Andrews

Center for Applied Energy Research Faculty and Staff Publications

CO₂ capture and recycle using microalgae was demonstrated at a coal-fired power plant (Duke Energy’s East Bend Station, Kentucky). Using an in-house designed closed loop, vertical tube photobioreactor, Scenedesmus acutus was cultured using flue gas as the CO₂ source. Algae productivity of 39 g/(m² day) in June–July was achieved at significant scale (18,000 L), while average daily productivity slightly in excess of 10 g/(m² day) was demonstrated in the month of December. A protocol for low-cost algae harvesting and dewatering was developed, and the conversion of algal lipids—extracted from the harvested biomass—to diesel-range hydrocarbons via catalytic …

Phanerochaete Chrysosporium Pretreatment Of Biomass To Enhance Solvent Production In Subsequent Bacterial Solid-Substrate Cultivation, Wanying Yao, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

This study investigated pretreatment of corn stover using solid substrate cultivation (SSC) of Phanerochaete chrysosporium (P. chrysosporium) to improve subsequent accessibility to cellulose. Thereafter, Clostridium thermocellum (C. thermocellum) was directly inoculated onto the pretreated biomass to accomplish hydrolysis, followed by solventogenesis by introducing Clostridium beijerinckii (C. beijerinckii).

An enzyme suite containing laccase, lignin peroxidase and manganese peroxidase activity was detected during the cultivation of P. chrysosporium on corn stover within 288 h at an initial moisture content 𝓌_H2O = 80% (mass of water/total mass). Incubation factors, such as substrate moisture content and cultivation …

Application Of The Kalman Filter On Full Tensor Gravity Gradiometry Data Around The Vinton Salt Dome, Louisiana, Mahnaz Sepehrmanesh

Theses and Dissertations--Earth and Environmental Sciences

Full tensor gravity (FTG) data are known for their high resolution but also for higher noise in its components due to the dynamic nature of the platform used for data acquisition. Although a review of the literature suggests steady increase in the success of gravity gradiometry, we still cannot take advantage of the full potential of the method, mostly because of the noise with the same amplitude and wavenumber characteristics as the signal that affects these data. Smoothing from common low pass filters removes small wavelength features and makes it difficult to detect structural features and other density variations of …

Soil And Biosolid Nano- And Macro-Colloid Properties And Contaminant Transport Behavior, Jessique L. Ghezzi

Theses and Dissertations--Plant and Soil Sciences

Despite indications that they are potential contaminant transport systems and threats to groundwater quality, very little effort has been invested in comparing contaminant transport behavior of natural environmental nanocolloids and their corresponding macrocolloid fractions in the presence of As, Se, Pb, and Cu contaminants. This study involved physico-chemical, mineralogical, stability and contaminant-transport characterizations of nano- (< 100 nm) and macro-colloids (100-2000 nm) fractionated from three Kentucky soils and one biosolid waste. Particle size was investigated with SEM/TEM and dynamic light scattering. Surface reactivity was estimated using CEC and zeta potential. Mineralogical composition was determined by XRD, FTIR, and thermogravimetric analyses. Sorption isotherms assessed affinities for Cu²⁺, Pb²⁺, AsO₃^-, and SeO₄^-2 contaminants, while settling kinetics experiments of suspensions at 0, 2 and 10 mg/L contaminants determined stability and transportability potential. Undisturbed 18x30 cm KY Ashton Loam soil monoliths were also used for …

Shallow Groundwater Monitoring Associated With The Deep Saline Reservoir Co2 Storage Test In Hancock County, Kentucky, E. Glynn Beck

Report of Investigations--KGS

A carbon dioxide injection test well was drilled in 2009 to a depth of 8,126 ft below ground surface in Hancock County, Kentucky. Six hundred ninety tons of CO₂ was successfully injected into Knox Group saline aquifers. Two domestic wells (MB and GB) and two domestic springs (CA and RC) were sampled to monitor any potential changes in groundwater quality associated with possible migration of CO₂ from deeper saline aquifers into shallow freshwater aquifers. The four sites were sampled for pH, bicarbonate, total dissolved solids, turbidity, anions, total dissolved metals, dissolved inorganic carbon, total CO₂, and …

Hydrogeologic Investigations Of Pavement Subsidence In The Cumberland Gap Tunnel, James S. Dinger, James C. Currens, Junfeng Zhu, Steven E. Webb, Brad W. Rister, R. C. Graves, David L. Allen, Tim C. Scully

Report of Investigations--KGS

Cumberland Gap Tunnel was constructed under Cumberland Gap National Historical Park in 1996 to improve transportation on a segment of U.S. 25E, connecting Kentucky and Tennessee and restoring Cumberland Gap to its historical appearance.

The concrete pavement in the tunnel started to subside in 2001. Ground penetrating radar surveys revealed voids in many areas of the limestone roadbed aggregate beneath the pavement. To investigate possible hydrogeologic processes that may have caused favorable conditions for voids to form in the aggregate, we studied geology, groundwater flow, and groundwater chemistry in the tunnel using a variety of methods, including bore drilling, packer …

Review Of Kentucky Oil And Gas Production, 2010, Brandon C. Nuttall

Information Circular--KGS

The 2010 oil and natural gas production data by well published by the Kentucky Division of Oil and Gas were analyzed in response to proposed rules for limiting emissions from oil and natural gas facilities. Average daily production was determined for 5,044 oil wells and 12,940 natural gas wells. Of these wells, 955 are combined oil and natural gas producers. The overwhelming majority of Kentucky’s wells are marginal (stripper) wells and produce at rates of less than 10 barrels of oil (or barrels of oil equivalent) per day (bo/d) or 60 thousand cubic feet of natural gas per day (Mcf/d) …

Kentucky Nutrient Model Calibration Tutorial, Kentucky Water Resources Research Institute

KWRRI Research Reports

This tutorial has been developed to illustrate how to calibrate the KYNM using two different example watersheds: 1) the Upper Chenoweth Run watershed and 2) the Middle Chenoweth Run watershed. Both models are provided with the KYNM Users Manual. The Upper Chenoweth Run does not contain any significant point sources, while the Middle Chenoweth Run watershed contains several point sources. Calibrating a model of a dynamic system is often not a simple linear process but an iterative one. The following discussion outlines “steps” in calibrating a model, but it must be understood that the phenomena being modeled are interconnected and …

The Kentucky Nutrient Model (Kynm), Kentucky Water Resources Research Institute

KWRRI Research Reports

The Kentucky Nutrient Model (KYNM) was developed in 2014 to provide the Kentucky Division of Water (KYDOW) with a simplified tool for use in developing nutrient based TMDLs and in evaluating different nutrient management strategies. This report provides a brief overview of the KYNM along with a discussion of the validation of the model against observed hydrology and water quality data, and a comparison of the model results against the results obtained by the LSPC model developed by Tetra Tech for the Floyds Fork Watershed.

Monitoring The Influence Of Acid Deposition On Soil And Implications To Forest Health In The Daniel Boone National Forest, Tyler M. Sanderson

Theses and Dissertations--Forestry and Natural Resources

Combustion of fossil fuels has contributed to many environmental problems including acid deposition. The Clean Air Act (CAA) was created to reduce ecological problems by cutting emissions of sulfur and nitrogen. Reduced emissions and rainfall concentrations of acidic ions have been observed since the enactment of the CAA, but soils continue to receive some acid inputs. Soils sensitive to acid deposition have been found to have low pH, a loss of base cations and a shift in the mineral phase controlling the activity of Al³⁺ and/or SO₄^2-. If inputs continue, soil may be depleted of base …

Community Visions For Nutrient Management For The Floyds Fork Watershed, Kentucky Water Resources Research Institute

KWRRI Research Reports

This report presents the results of a stakeholder engagement process for the Floyds Fork Watershed in north central Kentucky. The report is meant to inform the local citizens of the watershed, as well as US EPA and the Kentucky Division of Water as part of ongoing watershed management activities within the watershed. The process implemented in this study are intended to provide insights into a range of perspectives and community preferences related to possible nutrient management strategies for use in the Floyds Fork Watershed.

The Effects Of Mountaintop Removal Mining And Valley Fills On Stream Salamander Communities, Brenee' Lynn Muncy

Theses and Dissertations--Forestry and Natural Resources

Mountaintop removal mining and valley filling (MTR/VF) is a common form of land conversion in Central Appalachia and threatens the integrity of stream ecosystems. We investigated the effects of MTR/VF on stream salamander occupancy probabilities and community structure by conducting area constrained active searches for stream salamanders within intermittent streams located in mature forest (i.e., control) and those impacted by MTR/VF. During March to June of 2013, we detected five stream salamander species (Desmognathus fuscus, D. monticol, Eurycea cirrigera, Pseudotriton ruber, and Gyrinophilus porphyriticus) and found that the probability of occupancy was greatly reduced in MTR/VF streams compared …

Natural Phenomena As Potential Influence On Social And Political Behavior: The Earth’S Magnetic Field, Jackie R. East

Theses and Dissertations--Political Science

Researchers use natural phenomena in a number of disciplines to help explain human behavioral outcomes. Research regarding the potential effects of magnetic fields on animal and human behavior indicates that fields could influence outcomes of interest to social scientists. Tests so far have been limited in scope. This work is a preliminary evaluation of whether the earth’s magnetic field influences human behavior it examines the baseline relationship exhibited between geomagnetic readings and a host of social and political outcomes. The emphasis on breadth of topical coverage in these statistical trials, rather than on depth of development for any one model, …

A Summary Of The Kentucky River Watershed Watch 2014 Water Sampling Results, Kentucky Water Resources Research Institute

Kentucky River Watershed Watch

No abstract provided.

Addressing Public Health Risks Of Persistent Pollutants Through Nutritional Modulation And Biomimetic Nanocomposite Remediation Platforms, Bradley J. Newsome

Theses and Dissertations--Chemistry

Due to their relative chemical stability and ubiquity in the environment, chlorinated organic contaminants such as polychlorinated biphenyls (PCBs) pose significant health risks and enduring remediation challenges. Engineered nanoparticles (NPs) provide a novel platform for sensing/remediation of these toxicants, in addition to the growing use of NPs in many industrial and biomedical applications, but there remains concern for their potential long-term health effects. Research highlighted herein also represents a transdisciplinary approach to address human health challenges associated with exposure to PCBs and NPs. The objectives of this dissertation research are two-fold, 1) to develop effective methods for capture/sensing and remediation …

Improving Methods For The Successful Establishment Of Switchgrass, Whitney Marie Monin

Theses and Dissertations--Plant and Soil Sciences

Our research investigated whether priming switchgrass seeds with water or ethephon would increase stand establishment in the field. ‘Alamo’ seed germinated faster and grew taller than ‘Cave-in-Rock.Seeds primed for six days in water or for one day in ethephon 10 mM had the greatest seedling densities. In growth chamber environments seed priming were tested to hasten germination velocity. Seeds primed for two, four or six days in water germinated faster than unprimed seeds. Ethephon treatments reduced overall germination and germination velocity. Accent and Accent Q herbicides containing nicosulfuron are used to control weeds. To test ‘Alamo’ sensitivity to these herbicides, …

Environmental Justice In Kentucky: A County-Level Study Of Enforcement Of The Clean Air Act, Katherine Meade

MPA/MPP/MPFM Capstone Projects

Not available.