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Toxicokinetics And Metabolism Of Aniline And 4-Chloroaniline In Medaka (Oryzias Latipes), Steven P. Bradbury, Julia M. Dady, Patrick N. Fitzsimmons, Michelle M. Voit, Dean E, Hammermeister, Russell J. Erickson

Steven P. Bradbury

The in vivo toxicokinetics and in vitro hepatic microsomal metabolism of (14C)aniline and (14C)4-chloroaniline in medaka (Oryzias latipes) were investigated to provide a basis upon which to interpret the toxicological responses of small aquarium fish to aniline derivatives. During static aqueous exposures of up to 320 min, parent equivalents failed to reach steady state and results from depuration studies clearly demonstrated biphasic elimination. Due to low elimination rates, 40 to 20% of absorbed aniline and 4-chloroaniline doses, respectively, remained within the fish through 330 min postexposure. Based on an analysis of excreted metabolites, N-acetylation was the dominant route of in …