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Engineering Of Escherichia Coli 2-Oxoglutarate Dehydrogenase Complex With Mechanistic And Synthetic Goals, Joydeep Chakraborty
Engineering Of Escherichia Coli 2-Oxoglutarate Dehydrogenase Complex With Mechanistic And Synthetic Goals, Joydeep Chakraborty
Dissertations
The Escherichia coli 2-oxoglutarate dehydrogenase complex (OGDHc) compromises multiple copies of three enzymes - 2-oxoglutarate dehydrogenase (E1o), dihydrolipoyl succinyltransferase (E2o), and dihydrolipoyl dehydrogenase (E3). OGDHc is found in the Krebs cycle and catalyzes the formation of the all-important succinyl-Coenzyme A (succinyl-CoA). OGDHc was engineered to understand the catalytic mechanism and optimized for chemical synthetic goals.
Succinyl-CoA formation takes place within the catalytic domain of E2o via a transesterification reaction. The succinyl group from the thiol ester of S8-succinyldihydrolipoyl-E2o is transferred to the thiol group of CoA. Mechanistic studies were designed to investigate enzymatic transthioesterification. His375 and Asp374 was shown to …
Development Of Environmentally Benign Microencapsulation With Polymer Microspheres And Liposomes, Baohua Yue
Development Of Environmentally Benign Microencapsulation With Polymer Microspheres And Liposomes, Baohua Yue
Dissertations
Microencapsulation means applying a shell-like coating to encapsulate the contents of interest in a particle form with a size range of few micrometers or below. In this work, aqueous liposome systems and polymer based encapsulation of fine particles in supercritical CO2 were studied. Compared to many other microencapsulation methods, these two methodologies feature reduction/prevention of using organic solvents, making them particularly attractive as green technology.
For polymer microencapsulation, a novel in situ polymerization based process to encapsulate various types of fine particles, include drugs, fire retardant, inorganic nanoparticles, and carbon nanotubes, was developed. In the process, host particles, monomers …