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Environmental Sciences

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East Tennessee State University

Electronic Theses and Dissertations

Theses/Dissertations

Sinking Creek

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Bacterial Source Tracking In The Sinking Creek Watershed Using Antibiotic Resistance Analysis And Ribotyping., Lisa Kathleen Gallagher May 2008

Bacterial Source Tracking In The Sinking Creek Watershed Using Antibiotic Resistance Analysis And Ribotyping., Lisa Kathleen Gallagher

Electronic Theses and Dissertations

Fecal pollution of surface water is a significant environmental health issue. Indicator organisms are used to monitor microbial water quality, but often their presence does not coincide with the presence of pathogens. Bacterial source tracking is a term describing methods to determine the origin of fecal pollution based on bacterial traits. The objective of this research is to evaluate the use of 2 bacterial source tracking techniques, antibiotic resistance analysis (ARA) and ribotyping, to determine the sources of bacteria isolated from Sinking Creek. Based on the results of this study, ARA and ribotyping are not useful techniques for identifying sources …


The Use Of Selected Water Quality Parameters To Identify Fecal Coliform Sources In Support Of The Sinking Creek Total Maximum Daily Load., Susana Maria Floresguerra Dec 2003

The Use Of Selected Water Quality Parameters To Identify Fecal Coliform Sources In Support Of The Sinking Creek Total Maximum Daily Load., Susana Maria Floresguerra

Electronic Theses and Dissertations

Sinking Creek, located in upper east Tennessee, is on the 303(d) list for not meeting minimum water quality standards for recreation. A Total Maximum Daily Load (TMDL) for fecal coliforms was developed. The purpose of this study was to investigate the use of water quality parameters to identify areas that contribute to the fecal coliform loading. Concentrations of nitrate-N, orthophosphates, BOD, alkalinity, hardness, and optical brighteners (OB) were monitored at fourteen stations monthly for one year. Site 3 (agricultural region) exhibited the highest average nitrate-N loadings (627.34 mg/sec) and orthophosphate (as PO43-) loadings (84.83 mg/sec). Alkalinity loadings …