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Anaerobic Oxidation Of Methane At Different Temperature Regimes In Guaymas Basin Hydrothermal Sediments, Jennifer F. Biddle, Zena Cardman, Howard Mendlovitz, Daniel B. Albert, Karen G. Lloyd, Karen Lloyd, Antje Boetius, Andreas Teske
Anaerobic Oxidation Of Methane At Different Temperature Regimes In Guaymas Basin Hydrothermal Sediments, Jennifer F. Biddle, Zena Cardman, Howard Mendlovitz, Daniel B. Albert, Karen G. Lloyd, Karen Lloyd, Antje Boetius, Andreas Teske
Karen Lloyd
Anaerobic oxidation of methane (AOM) was investigated in hydrothermal sediments of Guaymas Basin based on d13C signatures of CH4, dissolved inorganic carbon and porewater concentration profiles of CH4 and sulfate. Cool, warm and hot in-situ temperature regimes (15–20 1C, 30–35 1C and 70–95 1C) were selected from hydrothermal locations in Guaymas Basin to compare AOM geochemistry and 16S ribosomal RNA (rRNA), mcrA and dsrAB genes of the microbial communities. 16S rRNA gene clone libraries from the cool and hot AOM cores yielded similar archaeal types such as Miscellaneous Crenarchaeotal Group, Thermoproteales and anaerobic methane-oxidizing archaea (ANME)-1; some of the ANME-1 …
Archaea Of The Miscellaneous Crenarchaeotal Group (Mcg) Are Abundant, Diverse, And Widespread In Marine Sediments, Kyoko Kubo, Karen Lloyd, Jennifer F. Biddle, Rudolf Amann, Andreas Teske, Katrin Knittel
Archaea Of The Miscellaneous Crenarchaeotal Group (Mcg) Are Abundant, Diverse, And Widespread In Marine Sediments, Kyoko Kubo, Karen Lloyd, Jennifer F. Biddle, Rudolf Amann, Andreas Teske, Katrin Knittel
Karen Lloyd
Members of the highly diverse Miscellaneous Crenarchaeotal Group (MCG) are globally distributed in various marine and continental habitats. In this study, we applied a polyphasic approach (rRNA slot blot hybridization, quantitative PCR (qPCR) and catalyzed reporter deposition FISH) using newly developed probes and primers for the in situ detection and quantification of MCG crenarchaeota in diverse types of marine sediments and microbial mats. In general, abundance of MCG (cocci, 0.4 lm) relative to other archaea was highest (12–100%) in anoxic, low-energy environments characterized by deeper sulfate depletion and lower microbial respiration rates (P¼0.06 for slot blot and P¼0.05 for qPCR). …