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Articles 1 - 9 of 9
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Functional Characterization Of Mycobacterium Smegmatis Phage Moomoo Gene Products: Identification Of Toxic Genes, Whitney Heard
Functional Characterization Of Mycobacterium Smegmatis Phage Moomoo Gene Products: Identification Of Toxic Genes, Whitney Heard
Masters Theses & Specialist Projects
Mycobacteriophages are a group of phages that infect members of the genus Mycobacteria. Previous studies have conducted extensive comparisons of the mycobacteriophage genomes and amino acid sequences to establish different phage families. MooMoo is a singleton mycobacteriophage that has been characterized due to its lack of appreciable homology to other phages. Some of its unique properties include its structure and the isolation of a mutant that causes clear plaque phenotypes. The experiments described in this thesis identified three MooMoo phage (gp87, gp90, and gp91) encoded proteins that are toxic to the bacterial host, Mycobacterium smegmatis.Through the use of a …
Properties Of A Genetically Unique Mycobacteriophage, Amanda K. Staples
Properties Of A Genetically Unique Mycobacteriophage, Amanda K. Staples
Masters Theses & Specialist Projects
Bacteriophage MooMoo is a temperate phage that was isolated and propagated on Mycobacterium smegmatis (M. smeg). It typically produces turbid plaques, however spontaneous clear plaque mutants can be readily isolated. Both turbid (MooMoo-T) and clear plaque (MooMoo-C) formers can establish stable lysogens, but the parental turbid plaque forming phage has a higher lysogenic frequency. The phage repressor protein typically plays the central role in regulating the lysis/lysogeny decision. Therefore, we expected that the mutation responsible for the clear plaque phenotype would be located in the repressor gene. Remarkably, whole genome sequencing detected a single base pair mutation in the minor …
Epiphytic Diatom Community Structure In A Karst Riverine System, Gregory John Barren
Epiphytic Diatom Community Structure In A Karst Riverine System, Gregory John Barren
Masters Theses & Specialist Projects
The goal of this study was to assess the epiphytic diatom community structure of two host species along a karst gradient in the upper Green River, Kentucky to a gain a better understanding of the role of diatoms in the food web. The host species studied were Podostemum ceratophyllum and Cladophora. Percent cover of P. ceratophyllum and Cladophora were quantified in the four study reaches. The host species were sampled near-shore and mid-channel in each reach in September and October of 2013. After diatoms were extracted from the host and enumerated the density and diversity were quantified. Twelve genera were …
Defining The Requirements For Early Gene Expression In Bacteriophage Hk639, Amanda L. Seaton
Defining The Requirements For Early Gene Expression In Bacteriophage Hk639, Amanda L. Seaton
Masters Theses & Specialist Projects
Lambdoid phages suppress transcription termination to fully express their genes. Antitermination of early gene expression in most lambdoid phages is mediated by an interaction between the N protein and a number of host-encoded factors. Bacteriophage HK022 does not rely on a protein for antitermination. To promote full expression of early phage genes, the transcripts of the HK022 put sites interact directly with RNA polymerase to convert it to a termination resistant form. Bacteriophage HK639 also uses RNA-mediated antitermination. However, it only possesses a single put-like element in its left operon. Because most lambdoid phages, including HK022, have antiterminator elements in …
Production And Degradation Of 4-Ethylphenol In Lactobacillus Sp. Pep8 Cultures And In Blended Swine Lagoon Enrichments, Clinton W. Copp
Production And Degradation Of 4-Ethylphenol In Lactobacillus Sp. Pep8 Cultures And In Blended Swine Lagoon Enrichments, Clinton W. Copp
Masters Theses & Specialist Projects
4-Ethylphenol (4-EP) is a malodorant of swine waste and is derived from a component of lignin called p-coumaric acid (p-CA). The production of 4-EP from lignin in swine waste is untested. Additionally, the effect of Fe (III) on 4-EP levels is unknown. Four experiments were performed to determine if Lactobacillus sp. pep8 cultures, as well as enriched swine lagoon slurries, could liberate p-CA from lignin and convert p-CA to 4-EP. Furthermore, it was tested if the addition of Fe (III) influences the conversion of p- CA to 4-EP.
Experiment 1 tested Lactobacillus sp. pep8 cultures to determine if the addition …
Ua3/9/5 Conservation Reserve Enhancement Program (Crep), Wku President's Office
Ua3/9/5 Conservation Reserve Enhancement Program (Crep), Wku President's Office
WKU Archives Records
Talking points used by WKU president Gary Ransdell regarding WKU's partnering with Conservation Reserve Enhancement Program.
A Modified Scheme For The Isolation And Enumeration Of Bacteria In Municipal Sewage Sludge, Kelly Ball
A Modified Scheme For The Isolation And Enumeration Of Bacteria In Municipal Sewage Sludge, Kelly Ball
Masters Theses & Specialist Projects
Because of the potential health hazards associated with the use of sludge for agricultural purposes, Dudley et al (1980) published a scheme for the routine analysis of bacteria in municipal sewage sludge. In this study, the Dudley et al scheme (1980) was modified by updating some of the procedures. Aerobically digested sludge generated by the Bowling Green Wastewater Treatment Plant, Bowling Green, Kentucky, was analyzed using the modified scheme. Sludge samples were collected once every two months over a one-year period from October 1989 to August 1990.
Egg yolk-free tryptose sulfite cycloserine agar in conjunction with the revewrse CAMP test …
Spatial Distribution & Temporal Occurrence Of Rotifers In The Main Pool & Tailwater Of Barren Lake, Kentucky, David Abel
Spatial Distribution & Temporal Occurrence Of Rotifers In The Main Pool & Tailwater Of Barren Lake, Kentucky, David Abel
Biology Graduate Theses
A study of the spatial distribution and temporal occurrence and diversity of rotifers in relation to certain chemicophysical parameters was conducted in Barren Lake, Kentucky, a flood control lake, from January, 1970, through January, 1970. Paired samples were collected from six depths in the main pool area of the reservoir and from an automatic plankton sampler installed in the tailwater area where, from April through September, the effluent was derived from the upper 3 m of the pool . Diel studies were conducted in April, July, 1970, and January, 1971.
Barren Lake began to stratify in late April, was completely …
Sanitation Of Drinking Water, Kentucky Library Research Collections
Sanitation Of Drinking Water, Kentucky Library Research Collections
Research Collections
The Arena was an American literary and political magazine founded by journalist, Benjamin Flower. Flower wanted the magazine to "agitate, educate, organize," he used investigative techniques to obtain information to bring about social, economic and political reform. This article focuses on the contamination of drinking water and its role in spreading diseases.