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Evaluating Edna Metabarcoding As A Mic-Roe-Scopic Net To Catch Salmon Pathogens, Noah Burby
Evaluating Edna Metabarcoding As A Mic-Roe-Scopic Net To Catch Salmon Pathogens, Noah Burby
Honors College
Wild Atlantic salmon in the Gulf of Maine (GOM) is a Distinct Population Segment (DPS) that has been listed since 2000 as endangered by the U.S. Fish and Wildlife Service (USFWS) and the National Oceanic and Atmospheric Administration (NOAA). The current challenge is year-over-year decreases in the number of mature salmon returning to the Penobscot River for reproduction. Early detection of pathogen presence could allow for the identification of infection and the application of corrective measures. Environmental DNA (eDNA) is simply DNA that is collected from environmental samples (e.g., water, air, and soils), which consists of whole microorganisms and genetic …
Urbanization Changes The Composition And Bioavailability Of Dissolved Organic Matter In Headwater Streams, Thomas B. Parr, Christopher Cronan, Tsutomu Ohno, Stuart Findlay, Sean Smith, Kevin Simon
Urbanization Changes The Composition And Bioavailability Of Dissolved Organic Matter In Headwater Streams, Thomas B. Parr, Christopher Cronan, Tsutomu Ohno, Stuart Findlay, Sean Smith, Kevin Simon
Publications
Population growth in cities has resulted in the rapid expansion of urbanized land. Most research and management of stream ecosystems affected by urban expansion has focused on the maintenance and restoration of biotic communities rather than their basal resources. We examined the potential for urbanization to induce bottom-up ecosystem effects by looking at its influence on dissolved organic matter (DOM) composition and bioavailability and microbial enzyme activity. We selected 113 headwater streams across a gradient of urbanization in central and southern Maine and used elemental and optical analyses, including parallel factor analysis of excitation-emission matrices, to characterize DOM composition. Results …
Comparafac: A Library And Tools For Rapid And Quantitative Comparison Of Dissolved Organic Matter Components Resolved By Parallel Factor Analysis., Thomas B. Parr, Tsutomu Ohno, Kevin Simon
Comparafac: A Library And Tools For Rapid And Quantitative Comparison Of Dissolved Organic Matter Components Resolved By Parallel Factor Analysis., Thomas B. Parr, Tsutomu Ohno, Kevin Simon
Publications
Parallel Factor Analysis (PARAFAC) is a well-established method for characterizing dissolved organic matter (DOM). While methods for sample processing and PARAFAC analysis are well defined and robust, subsequent classification of DOM fluorescence components and comparisons of components among studies remain highly qualitative. Because these comparisons often guide the interpretation of subsequent data, it is important that quantitatively accurate comparisons be made. We developed a statistical tool, comPARAFAC, using a modified Tucker’s Congruence Coefficient (mTCC), an established method of factor comparison, to provide a quantitative basis for comparing models. To develop and test this tool we used mTCC to compare factors …
U.S.-Korea Cooperative Research: Carbon Monoxide As A Substrate For Microbial Maintenance, Gary M. King
U.S.-Korea Cooperative Research: Carbon Monoxide As A Substrate For Microbial Maintenance, Gary M. King
University of Maine Office of Research Administration: Grant Reports
Bacteria play an important role in the global budget of carbon monoxide (CO). Largely unknown bacterial populations in soils and the water column of aquatic systems oxidize hundreds of teragrams per year, or about 10%-20% of the estimated annual flux to the atmosphere. In spite of their biogeochemical significance, relatively little is known about the identity of CO-oxidizing populations active in situ, their phylogenic and physiological diversity or the importance of CO as substrate for their basic metabolic needs. of CO oxidizers. It is clear that CO at high concentrations (> 1000 ppm) can serve as a sole source of …
Microbial Observatories: Kilauea Volcano Observatory For Carbon Monoxide-Oxidizing Bacteria, Gary M. King
Microbial Observatories: Kilauea Volcano Observatory For Carbon Monoxide-Oxidizing Bacteria, Gary M. King
University of Maine Office of Research Administration: Grant Reports
Volcanic activity regularly creates new landforms from deposits of tephra, ash and lava. These initially sterile, pristine deposits undergo a range of physical, chemical and biological transformations that lead in some cases to diverse, complex ecosystems such as Hawaiian rainforests. Recent activity by the Kilauea volcano has created unique opportunities to understand the timing and controls of complex ecosystem development, and to analyze the roles of microbes as pioneering colonists that contribute to plant development and succession. The newly established Kilauea Volcano Microbial Observatory will compare the diversity and activity of carbon monoxide-oxidizing bacteria colonizing two different deposits currently 45 …