Digital Commons Network^™

Long Time No See: New Reports Of Legua Rosea Amédégnato And Poulain, 1986 (Orthoptera: Caelifera: Romaleidae: Romaleinae: Leguini), A Rare Arboreal Grasshopper From Brazil, Daniela Santos Martins Silva, Renan Da Silva Olivier, Larissa Lima De Queiroz, Marcelo Ribeiro Pereira, Jardel Boscardin, Guilherme Rabelo D’Angelis

Insecta Mundi

The genus Legua Walker, 1870 comprises only two known species from Central America and Brazil, with a notable scarcity of collected specimens. Herein, we provide a new contribution to our knowledge of the distribution of Legua rosea Amédégnato and Poulain, 1986 (Romaleinae: Leguini), that has a restricted distribution in Brazil, based on entomological collection data and social media information. Our records emphasize the importance of natural history collections and new tools for biodiversity studies.

ZooBank registration. urn:lsid:zoobank.org:pub:17E0802B-A389-4983-AC74-21B51197F557

First Record Of The Invasive Chenopodium Aphid, Hayhurstia Atriplicis (Linnaeus, 1761) (Hemiptera: Aphididae), In Colombia, Takumasa Kondo, Roberto Rosero, Jackeline Gaviria

Insecta Mundi

The chenopodium aphid, Hayhurstia atriplicis (Linnaeus, 1761) (Hemiptera: Aphididae), is reported for the first time in Colombia. The aphid was found on the aerial parts of the quinoa plant, Chenopodium quinoa Willd. (Amaranthaceae), especially on the leaves, in five municipalities in the department of Cauca, Colombia. Brief information on its natural enemies, distribution and biology is provided. This is the only aphid in Colombia known to cause malformations on the leaves of quinoa plants. In South America, this aphid has been found previously only in Ecuador, where it was reported for the first time in 2023 based on specimens collected …

Can The Vegetation Structure And Composition In Urban Green Spaces Determine Diversity Of Green Lacewings (Neuroptera: Chrysopidae)?, Jose I. Martinez, Raiza J. Castillo, Adrián Ardila-Camacho, Charles V. Covell, José Isabel López-Arroyo, Francisco Javier Nava-Guízar

Insecta Mundi

Green spaces represent the only natural areas in several cities around the world, providing good shelters for the local fauna. Based on this premise, many ecological studies have been conducted focused on these areas. Most of these works are about insects, particularly butterflies and beetles. Our study is centered on a different group: green lacewings (Neuroptera: Chrysopidae). These insects exhibit a similar feeding behavior to some other groups, such as beetles. We estimated diversity, richness, distribution, abundance and similarity employing two methods: sweep netting and suction trapping. Also, oviposition hosts were identified in 20 different green spaces. Approximately 740 specimens …

Biodiversity Of Histerid Beetles (Coleoptera: Histeridae) From Brazil. I. Southern Region, Fábio Luiz De Leão Leonel, Fernando Willyan Trevisan Leivas

Insecta Mundi

This contribution adds data to the conservation and distribution of Histeridae species (Coleoptera) in the Neotropical region through a survey of Brazil’s South region. More specifically, it provides: i) a list of local Histeridae species; ii) a list of species that are inside Conservation Units in southern Brazil; iii) biotic (biome, ecoregion and associations/affinities) and abiotic information (altitude and month of occurrence) for each species. In total, 66 genera and 164 histerid beetle species were recorded, distributed in seven subfami­lies and 11 tribes. Among these, one genus and three species are new records for the region. Fifty percent of the …

A New Species Of Epeolus Latreille, 1802 (Hymenoptera: Apidae) From Western North America, Thomas M. Onuferko, Cory S. Sheffield

Insecta Mundi

A new species of Epeolus Latreille, 1802 (Hymenoptera: Apidae)—E. emiliae Onuferko and Shef­field, new species—is described from North America west of the Great Plains. It is morphologically most similar to Epeolus autumnalis Robertson, 1902, a species known exclusively from east of the Rocky Moun­tains. DNA barcode sequences from representatives of E. autumnalis and E. emiliae share a barcode index number (i.e., BIN: BOLD:AAF2361), but the two species exhibit marked and consistent differences in integu­ment coloration and the patterns of pubescence on the metasoma, and their distributional ranges, based on known specimens, show no overlap. This discovery increases …

Stink Bug (Hemiptera: Heteroptera: Pentatomidae) Ecology In Nebraska Agroecosystems, Blessing Ademokoya

Department of Entomology: Dissertations, Theses, and Student Research

Stink bugs (Hemiptera: Heteroptera: Pentatomidae) have gained considerable attention in Nebraska in the last decade due to increasing densities of native stink bug and spread of invasive species in the Midwest. Little is known about stink bug dynamics in Nebraska cropping systems. Based on data from a recent field survey, specimens at the University of Nebraska State Museum and the diagnostic lab of the Entomology Department at the University of Nebraska Lincoln, as well as published literature, we present a checklist of 72 species and subspecies of Pentatomidae (55 Pentatominae, 13 Asopinae, 3 Podopinae and 1 Edessinae) that occur in …

First Record Of Orsilochides Scurrilis (Stål) (Hemiptera: Heteroptera: Scutelleridae: Pachycorinae) In The United States, With Notes On The Biology And Distribution Of U.S. Species Of Orsilochides Kirkaldy, A. W. Meeds, Joseph E. Eger Jr., S. D. Carnahan

Insecta Mundi

Orsilochides scurrilis (Stål) (Hemiptera: Heteroptera: Scutelleridae) is reported from the United States for the first time based on a specimen collected in Santa Cruz County, Arizona. A key to separate the U.S. species of Orsilochides Kirkaldy is provided. In addition, host plant records and distribution of the other two species of Orsilochides that occur in the U.S., Orsilochides guttata (Herrich-Schäffer) and Orsilochides stictica (Dallas), are analyzed through a combination of digital photo records and museum specimens.

Host-Use Patterns Of Canopy-Inhabiting Click Beetles (Coleoptera: Elateridae) In A Lowland Rainforest In Southern Venezuela, Susan Kirmse, Paul J. Johnson

Insecta Mundi

The arboreal click beetle fauna (Coleoptera: Elateridae) in a lowland tropical rainforest in south­ern Venezuela was observed and collected by means of a tower crane for a full year. The evaluation of the elaterid assemblage is part of a general survey of Coleoptera associated with several canopy trees. The Elat­eridae represented the tenth most species-rich beetle family in the canopy of the crane plot and was therefore selected for a detailed analysis of host-use patterns. In total, 20 species of Elateridae with 402 adult indi­viduals were sampled, including seven singletons. Species were either flower visiting (Aeolus Eschscholtz and Cosmesus …

A Deeper Meaning For Shallow-Level Phylogenomic Studies: Nested Anchored Hybrid Enrichment Offers Great Promise For Resolving The Tiger Moth Tree Of Life (Lepidoptera: Erebidae: Arctiinae), Nicolas J. Dowdy, Shannon Keating, Alan R. Lemmon, Emily M. Lemmon, William E. Conner, Clare H. Scott Chialvo, Susan Weller, Rebecca B. Simmons, Melissa S. Sisson, Jennifer M. Zaspel

Department of Entomology: Faculty Publications

Anchored hybrid enrichment (AHE) has emerged as a powerful tool for uncovering the evolutionary relationships within many taxonomic groups. AHE probe sets have been developed for a variety of insect groups, though none have yet been shown to be capable of simultaneously resolving deep and very shallow (e.g., intraspecific) divergences. In this study, we present NOC1, a new AHE probe set (730 loci) for Lepidoptera specialized for tiger moths and assess its ability to deliver phylogenetic utility at all taxonomic levels. We test the NOC1 probe set with 142 individuals from 116 species sampled from all the major lineages of …

Surveillance Of Culex And Aedes Mosquitoes In Lincoln, Lancaster County, Nebraska, William Noundou

Department of Entomology: Dissertations, Theses, and Student Research

In 2018, West Nile virus (WNV) was identified as the leading cause of mosquito-borne disease in the continental United States. In response to this very serious problem, the Lincoln-Lancaster County Public Health Department (LLCHD) reinforced their mosquito surveillance program, which constitutes one of the best available tools to fight against this serious threat to human health. The objectives of this study were to 1) expand knowledge of the activity and relative abundance of mosquito communities in understudied areas and 2) evaluate differences in mosquito communities by urban and rural location, especially focusing on known vector species. A total of 6 …

Surveillance Of Culex And Aedes Mosquitoes In Lincoln, Lancaster County, Nebraska, William Noundou

Department of Entomology: Dissertations, Theses, and Student Research

In 2018, West Nile virus (WNV) was identified as the leading cause of mosquito-borne disease in the continental United States. In response to this very serious problem, the Lincoln-Lancaster County Public Health Department (LLCHD) reinforced their mosquito surveillance program, which constitutes one of the best available tools to fight against this serious threat to human health. The objectives of this study were to 1) expand knowledge of the activity and relative abundance of mosquito communities in understudied areas and 2) evaluate differences in mosquito communities by urban and rural location, especially focusing on known vector species. A total of 6 …

Molecular Gut-Content Analysis Of Adult Ground Beetles (Coleoptera: Carabidae) Provides No Evidence Of Predation Of Western Corn Rootworm (Coleoptera: Chrysomelidae) In A Nebraska Corn Agroecosystem, Camila Oliveira-Hofman, Vinícius S. Victor, Lance Meinke, Julie A. Peterson

Department of Entomology: Faculty Publications

This study was conducted to characterize the ground beetle (Coleoptera: Carabidae) community in Nebraska continuous cornfields and investigate the potential for predation of the western corn rootworm, Diabrotica virgifera virgifera LeConte, a key rootfeeding insect pest that is an annual management challenge in this system. Seven collection dates were conducted at five commercial cornfields in west central Nebraska during the growing season of 2014. In each field, carabids were sampled using five 24-h pitfall traps. Carabid specimens were placed in 95% ethanol in the field and stored at –20 °C to preserve DNA. After identification to the species level, DNA …

The Trichoptera Of Panama X. The Quebrada Rambala Drainage, With Description Of 19 New Species Of Microcaddisflies (Trichoptera: Hydroptilidae), Steven C. Harris, Brian J. Armitage

Insecta Mundi

The Quebrada Rambala drainage is found immediately south and south-southeast of the town of Chiriqui Grande and east of Rambala on the Caribbean coast. It is one of two tributaries of the Rio Margarita watershed, a small, lowland drainage with elevations up to 180 m. During the 2014-2017 period, collections of caddisflies (Insecta: Trichoptera), employing both UV-light and Malaise traps, were made at two locations on Quebrada Rambala proper, and four locations on its unnamed tributary, all on a land area of approximately 1 ha. As a result, 127 species of caddisflies were identified, including 59 species of microcaddisflies. A …

Science At Engineer Cantonment, Hugh H. Genoways, Brett C. Ratcliffe, Carl R. Falk, Thomas E. Labedz, Paul R. Picha, John R. Bozell

University of Nebraska State Museum: Mammalogy Papers

Conclusions

It is our contention that Thomas Say, Titian Peale, Edwin James, and their colleagues of the Stephen Long Expedition of 1819–1820 were heavily engaged in scientific research, which took the form of the first biodiversity inventory undertaken in the United States. This accomplishment has been overlooked both by biologists and historians, but it should rank among the most significant accomplishments of the expedition. The results of this inventory continue to inform us today about environmental, faunal, and floral changes along the Missouri River in an area that is known to be an ecotone between the deciduous forests of the …

Using Publicly Available Data To Quantify Plant–Pollinator Interactions And Evaluate Conservation Seeding Mixes In The Northern Great Plains, Clint R.V. Otto, S. O'Dell, R. B. Bryant, N. H. Euliss Jr., R. M. Bush, Matthew D. Smart

Department of Entomology: Faculty Publications

Concern over declining pollinators has led to multiple conservation initiatives for improving forage for bees in agroecosystems. Using data available through the Pollinator Library (npwrc.usgs.gov/pollinator/), we summarize plant–pollinator interaction data collected from 2012–2015 on lands managed by the U.S. Fish and Wildlife Service and private lands enrolled in U.S. Department of Agriculture conservation programs in eastern North Dakota (ND). Furthermore, we demonstrate how plant–pollinator interaction data from the Pollinator Library and seed cost information can be used to evaluate hypothetical seeding mixes for pollinator habitat enhancements. We summarize records of 314 wild bee and 849 honey bee (Apis mellifera …

Integration Of Plant Defense Traits With Biological Control Of Arthropod Pests: Challenges And Opportunities, Julie A. Peterson, Paul J. Ode, Camila Oliveira-Hofman, James D. Harwood

Department of Entomology: Faculty Publications

Crop plants exhibit a wide diversity of defensive traits and strategies to protect themselves from damage by herbivorous pests and disease. These defensive traits may be naturally occurring or artificially selected through crop breeding, including introduction via genetic engineering. While these traits can have obvious and direct impacts on herbivorous pests, many have profound effects on higher trophic levels, including the natural enemies of herbivores. Multi-trophic effects of host plant resistance have the potential to influence, both positively and negatively, biological control. Plant defense traits can influence both the numerical and functional responses of natural enemies; these interactions can be …

Export Of Insect Ecosystem Services From Hayed Native Prairie, Wayne J. Ohnesorg

Department of Entomology: Dissertations, Theses, and Student Research

Natural areas, such as prairie, have the potential to provide the benefits of pest suppression and pollination for agricultural production. In Nebraska, prairie is often used for hay production. The impact of prairie hay production management practices on beneficial arthropods is not well understood. Four prairie hay meadows adjacent to row crop fields were selected in northeast Nebraska. The goal was to assess the impact of haying them on beneficial arthropods and the movement of natural enemies into adjacent crop fields. Three management practices were evaluated for hay harvested from prairie meadows one cut per growing season, two cuts per …

Stress-Induced Changes In Abundance Differ Among Obligate And Facultative Endosymbionts Of The Soybean Aphid, Laramy S. Enders, Nicholas Miller

Department of Entomology: Faculty Publications

Bacterial endosymbionts can drive evolutionary novelty by conferring adaptive benefits under adverse environmental conditions. Among aphid species there is growing evidence that symbionts influence tolerance to various forms of stress. However, the extent to which stress inflicted on the aphid host has cascading effects on symbiont community dynamics remains poorly understood. Here we simultaneously quantified the effect of host-plant induced and xenobiotic stress on soybean aphid (Aphis glycines) fitness and relative abundance of its three bacterial symbionts. Exposure to soybean defensive stress (Rag1 gene) and a neurotoxic insecticide (thiamethoxam) substantially reduced aphid composite fitness (survival 9 reproduction) …

Short-Horned Grasshopper Subfamilies Feed At Different Rates On Big Bluestem And Switchgrass Cultivars, Muhammad Ullah, Fatima Mustafa, William W. Hoback, Mathew L. Brust, Kerri M. Farnsworth-Hoback, John E. Foster

Department of Entomology: Faculty Publications

Grasshopper species belonging to subfamilies Melanoplinae, Gomphocerinae and Oedipodinae were tested for their feeding rate on three types of grass. All grasshopper species were offered Shawnee and Kanlow cultivars of switchgrass, Panicum virgatum L. and big bluestem, Andropogon gerardii Vitman. The grasshoppers, Melanoplus femurrubrum and Melanoplus differentialis were also tested for their feeding on turgid or wilted leaves of the Shawnee cultivar of switchgrass. We found that M. differentialis consumed more switchgrass compared to big bluestem while M. femurrubrum and Arphia xanthoptera consumed the most Shawnee switchgrass. The M. differentialis consumed more turgid grass compared to wilted switchgrass. The feeding …

The Beetles Of The Lesser Antilles (Insecta, Coleoptera): Diversity And Distributions, Stewart B. Peck

Insecta Mundi

The island arc of the Lesser Antilles lies at the eastern margin of the Caribbean Sea in the Western Hemisphere, and stretches from the eastern end of the islands of the Greater Antilles (at the Virgin Islands), south to a position near the continental islands of Trinidad and Tobago at the north eastern corner of South America. The islands are a part of the West Indian Islands biodiversity “hotspot” and have been available for terrestrial colonization for about the past 15 million years. This is a status report on present knowledge of the beetle faunas of these islands, which is …

Development Modeling Of Lucilia Sericata And Phormia Regina (Diptera: Calliphoridae), Amanda L. Roe

School of Natural Resources: Dissertations, Theses, and Student Research

The relationship between insect development and temperature has been well established and has a wide range of uses, including using blow flies for postmortem (PMI) interval estimations in death investigations. To use insects in estimating PMI, we must be able to determine the insect age at the time of discovery and backtrack to time of oviposition. Unfortunately, existing development models of forensically important insects are only linear approximations and do not take into account the curvilinear properties experienced at extreme temperatures. A series of experiments were conducted with two species of forensically important blow flies (Lucilia sericata and Phormia …

Minute Pirate Bug (Orius Insidiosus Say) Populations On Transgenic And Non-Transgenic Maize Using Different Sampling Techniques, Santiago A. Palizada, Bamphitlhi Tiroesele, Difabachew B. Kondidie, Muhammad Irfan Ullah, Fatima Mustafa, Thomas E. Hunt, Pete L. Clark, Jaime Molina-Ochoa, Steven R. Skoda, John E. Foster

Department of Entomology: Faculty Publications

Field experiments were conducted to evaluate the populations of minute pirate bug [Orius insidiosus (Say)] using visual, sticky cards and destructive sampling techniques in transgenic and non-transgenic maize in three locations in Nebraska (Mead, Clay Center. and Concord) United States of America. during 2007 and 2008 . All sampling methods revealed significant counts of O. insidiosus on CP4 EPSPS maize plus an insecticide application for control of first generation O. nubilalis at R2 (blister) sampling period. Similarly, visual observations of O. insidiosus on Cry1Ab x Cry3Bb1 x CP4 EPSPS maize yielded significantly higher mean adult counts at R2 (blister) …

Diversification Under Sexual Selection: The Relative Roles Of Mate Preference Strength And The Degree Of Divergence In Mate Preferences, Rafael L. Rodríguez, Janette W. Boughman, David A. Gray, Eileen A. Hebets, Gerlinde Höbel, Laurel B. Symes

Eileen Hebets Publications

The contribution of sexual selection to diversification remains poorly understood after decades of research. This may be in part because studies have focused predominantly on the strength of sexual selection, which offers an incomplete view of selection regimes. By contrast, students of natural selection focus on environmental differences that help compare selection regimes across populations. To ask how this disparity in focus may affect the conclusions of evolutionary research, we relate the amount of diversification in mating displays to quantitative descriptions of the strength and the amount of divergence in mate preferences across a diverse set of case studies of …

Non Target Effect Of Cry1 Ab And Cry Ab X Cry3 Bb1 Bt Transgenic Maize On Orius Insidiosus (Hemiptera: Anthocoridae) Abundance, Santiago A. Palizada, Difabachew K. Belay, Bamphitlhi Tiroesele, Fatima Mustafa, Muhammad Ullah, Thomas Hunt, Jaime Molina-Ochoa, S. R. Skoda, Pete L. Clark, John E. Foster

Department of Entomology: Faculty Publications

Non-target effects of Cry1Ab x CP4 EPSPS and Cry1Ab+Cry3Bb1×CP4 EPSPS Bt transgenic new maize hybrids on insidious flower bugs [Orius insidiosus (Say)] was studied in Nebraska (Mead, C lay Center, and Concord) during 2007 and 2008. The Bt effect was compared to CP4 EPSPS maize (isoline), conventional maize, and insecticide applications of permethrin (Pounce® 1.5G) and bifenthrin (Capture® 2EC) to control first and second generations of Ostrinia nubilalis (Hübner), respectively. Yellow sticky cards, visual observations, and destructive samplings were used to evaluate O. insidiosus abundance. The yellow sticky card data in 2007 showed that O. insidiosus abundance was lower …

Floral Associations Of Cyclocephaline Scarab Beetles, Matthew Robert Moore, Mary Liz Jameson

University of Nebraska State Museum: Entomology Papers

The scarab beetle tribe Cyclocephalini (Coleoptera: Scarabaeidae: Dynastinae) is the second largest tribe of rhinoceros beetles, with nearly 500 described species. This diverse group is most closely associated with early diverging angiosperm groups (the family Nymphaeaceae, magnoliid clade, and monocots), where they feed, mate, and receive the benefit of thermal rewards from the host plant. Cyclocephaline floral association data have never been synthesized, and a comprehensive review of this ecological interaction was necessary to promote research by updating nomenclature, identifying inconsistencies in the data, and reporting previously unpublished data. Based on the most specific data, at least 97 cyclocephaline beetle …

Comparison Of Three Collection Techniques For Capture Of Coleoptera, With An Emphasis On Saproxylic Species, In Great Smoky Mountains National Park, Usa, Michael L. Ferro, Matthew L. Gimmel, Kyle E. Harms, Christopher E. Carlton

Insecta Mundi

Collection methods and/or habitats sampled influence how many and which species are captured during entomological surveys. Here we compare Coleoptera catches among three survey activities, each using a single collection method, at the same study sites in Great Smoky Mountains National Park, USA. Activities included: short-term flight intercept trapping (FITs); sifting/Berlese funneling of leaf litter and extremely decayed downed coarse woody debris; and using emergence chambers containing coarse woody debris of various decay classes. In total, 2472 adult beetle specimens, representing 217 lowest identifiable taxa within 164 genera and 42 families, were collected during the FIT survey. Each survey activity …

An Annotated List Of Symmachia Hübner, [1819] (Lepidoptera: Riodinidae: Symmachiini) From Parque Nacional Da Serra Do Divisor, Acre, Brazil, With The Description Of A New Species, Diego Rodrigo Dolibaina, Luis Anderson Ribeiro Leite, Fernando Maia Silva Dias, Olaf Hermann Hendrik Mielke, Mirna Martins Casagrande

Insecta Mundi

We provide an illustrated list of species belonging to the genus Symmachia Hübner, [1819] (Lepidoptera: Riodinidae: Symmachiini) collected during an expedition conducted between September 10-21, 2011 in the northern part of the Parque Nacional da Serra do Divisor, Acre, Brazil, a remote region of Amazon rainforest. A total of 46 individuals were collected belonging to 15 species. For all recorded species, drawings of male genitalia and behavioral information are provided to support future studies on the taxonomy of the group. A new species, Symmachia divisora Dolibaina and Leite, sp. nov., is described, and a revised status for Symmachia hetaerina hesione …

Seasonal Population Dynamics Of The Potato Psyllid (Hemiptera: Triozidae) And Its Associated Pathogen “Candidatus Liberibacter Solanacearum” In Potatoes In The Southern Great Plains Of North America, John A. Goolsby, John J. Adamczyk Jr., J. M. Crosslin, Noel N. Troxclair, J. R. Ancisco, Gerhard G. Bester, J. D. Bradshaw, Edsel D. Bynum Jr., L. A. Carpio, Don C. Henne, Ankush Joshi, Joseph E. Munyaneza, Pat Porter, Phillip E. Sloderbeck, J. R. Supak, C. M. Rush, F. J. Willett, B. J. Zechmann, B. A. Zens

Department of Entomology: Faculty Publications

The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), and its associated pathogen “Candidatus Liberibacter solanacearum” (Ca. L. solanacearum), the putative causal agent of zebra chip (ZC) disease in potatoes (Solanum tuberosum L.), were sampled in commercial potato fields and untreated control plots for 3 yr in multiple locations in Texas, Kansas, Nebraska, and Colorado. Populations of the potato psyllid varied across years and across potato growing regions. However, the percentage of potato psyllids infected with Ca. L. solanacearum although variable across years, was consistently highest in the Lower Rio Grande Valley of Texas (LRGV), the reported overwintering …

Transmission Of Triticum Mosaic Virus And Its Impact On The Biology Of The Wheat Curl Mite Aceria Tosichella Keifer (Eriophyidae), And An Evaluation Of Management Tactics For The Wheat Curl Mite And The Wheat-Mite-Virus Complex, Anthony J. Mcmechan

Department of Entomology: Dissertations, Theses, and Student Research

The wheat-mite-virus complex is an important production constraint to winter wheat production in the Great Plains, and consists of three viruses; wheat streak mosaic (WSMV), wheat mosaic (WMoV) and Triticum mosaic virus (TriMV). Synergistic interactions between these viruses have resulted in increased rates of replication and transmission of viruses, thus increasing the potential impact on wheat yields. The wheat curl mite (WCM), Aceria tosichella Keifer is the only known vector of the viruses within the wheat-mite-virus complex.

Currently, three colonies of WCM have been characterized by differential responses to mite resistant genes (biotypes) in wheat and differential transmission of WMoV. …

Parochlus Kiefferi (Garrett, 1925) In Nebraska (Diptera: Chironomidae), Barbara Hayford

Great Plains Research: A Journal of Natural and Social Sciences

A rare species of nonbiting aquatic midge, Parochlus kiefferi (Garrett, 1925), was discovered in Squaw Creek in the Pine Ridge of northwest Nebraska. Parochlus is a genus of midge found throughout the Southern Hemisphere and is only represented by this one species in the Northern Hemisphere. The typical North American species distribution of P. kiefferi includes high alpine and northern latitude streams, so the collection of P. kiefferi from a low elevation and low-latitude stream in Nebraska represents a range extension for the species. A survey for P. kiefferi from 83 samples from 53 stream sites in northern Nebraska yielded …