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Purification And Characterization Of Cytoplasmic Nadp+- Isocitrate Dehydrogenase, And Amplification Of The Nadp+-Idh Gene From The Wing-Dimorphic Sand Field Cricket, Gryllus Firmus, Anthony J. Zera, Susan Newman, David Berkheim, Christine Black, Lindsay Klug, Erica Crone
Purification And Characterization Of Cytoplasmic Nadp+- Isocitrate Dehydrogenase, And Amplification Of The Nadp+-Idh Gene From The Wing-Dimorphic Sand Field Cricket, Gryllus Firmus, Anthony J. Zera, Susan Newman, David Berkheim, Christine Black, Lindsay Klug, Erica Crone
School of Biological Sciences: Faculty Publications
Cytoplasmic NADP+-isocitrate dehydrogenase (NADP+-IDH) has been purified and characterized, and its gene sequenced in many animal, plant, and yeast species. However, much less information is available on this enzyme-gene in insects. As a first step in investigating the biochemical and molecular mechanisms by which NADP+-IDH contributes to adaptations for flight vs. reproduction in insects, the enzyme was purified to homogeneity in the wing-dimorphic cricket, Gryllus firmus, characterized, and its corresponding gene sequenced. Using a combination of polyethylene glycol precipitation, Cibacron-Blue affinity chromatography, and hydrophobic interaction chromatography the enzyme was purified 291-fold (7% yield; specific activity = 15.8 μmol NADPH/min/mg …