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Improved Molecular Detection Tools For The Invasive Crop Pest Helicoverpa Armigera (Hübner), Mitchell D. Rich
Improved Molecular Detection Tools For The Invasive Crop Pest Helicoverpa Armigera (Hübner), Mitchell D. Rich
All Theses
Helicoverpa armigera is a major crop pest native to Europe, Asia, Australia, and Africa. H. armigera has recently invaded South America and has caused billions of dollars in agricultural losses. It is difficult to differentiate H. armigera from H. zea, a closely-related species native to North and South America. A few genetic tests have been previously developed to detect H. armigera DNA in pooled samples of moth legs. In this study, an improved qPCR melt curve assay with higher detection sensitivity and a field-based recombinase polymerase amplification (RPA) assay were developed for specific detection of H. armigera DNA …
Treatment Thresholds For Bollworm (Helicoverpa Zea) In Dual-Gene Bt Cotton, Kristen Carter
Treatment Thresholds For Bollworm (Helicoverpa Zea) In Dual-Gene Bt Cotton, Kristen Carter
All Theses
Dual-gene Bt cotton has reduced the need for insecticide treatments for bollworm, Helicoverpa zea (Boddie), compared with original single-gene Bt technology. Bollgard II¨ (Monsanto, St. Louis, MO) and WideStrike¨ (Dow AgroSciences, Indianapolis, IN), both produce the Cry1Ac protein and a second protein, Cry2Ab or Cry1F, respectively. These dual-gene Bt cottons provide enhanced control of lepidopteran pests, but remain less than 100% effective against bollworm, particularly when population pressure is high. Current recommended treatment thresholds for bollworm on cotton in South Carolina are as follows: treat with insecticides when three or more large larvae are found per 100 plants or when …