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Identification Of Giardia Lamblia-Specific Antigens In Infected Human And Gerbil Feces By Western Immunoblotting, Henry H. Stibbs, Mansour Samadpour, Jerry E. Ongerth
Identification Of Giardia Lamblia-Specific Antigens In Infected Human And Gerbil Feces By Western Immunoblotting, Henry H. Stibbs, Mansour Samadpour, Jerry E. Ongerth
Faculty of Engineering and Information Sciences - Papers: Part A
Western immunoblot analysis of aqueous extracts of feces obtained from five giardiasis patients and from experimentally infected gerbils (Meriones unguiculatus) with rabbit antiserum to Giardia lamblia cysts has revealed antigens of three molecular weight groups. A stepladderlike, evenly-spaced set of strongly reactive antigens (darkest at a molecular weight [m.w.] of 55,000 to 70,000) appeared in the gerbil feces from day 4 (first experiment) or day 2 (second experiment) and lasted to about day 7 but disappeared completely by day 8 and did not reappear later. These antigenic bands were seen in gerbils infected with two isolates of G. lamblia. These …