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Resistome And Mobilome In Surface Runoff From Manured Soil As Affected By Setback Distance, Noelle Mware, Maria C. Hall, Selvakumar Rajendran, John E. Gilley, Amy Schmidt, Shannon Bartelt-Hunt, Yifan Zhang, Xu Li

Department of Biological Systems Engineering: Papers and Publications

Land application of livestock manure introduces antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) into the soil environment. The objectives of this study were to examine the changes of resistome and mobilome in runoff and soil as a function of setback distance, i.e., the distance between manured soil and surface water, and to quantify the contributions of manure and background soil to the ARGs and MGEs in surface runoff. The resistome and mobilome in runoff and soil from a field-scale plot study were characterized using a high throughput quantitative polymerase chain reaction (HT-qPCR) array. It was estimated that a …

Differential Survival Of Non-O157 Shiga Toxigenic Escherichia Coli In Simulated Cattle Feedlot Runoff, Lisa M. Durso, John E. Gilley, Daniel N. Miller

Department of Biological Systems Engineering: Papers and Publications

Environmental survival time is important when evaluating adverse health outcomes from foodborne pathogens. Although outbreaks associated with manure-impacted irrigation or runoff water are relatively infrequent, their broad scope, regulatory importance, and severe health outcomes highlight the need to better understand the environmental survival of manure-borne pathogens. Shiga toxigenic Escherichia coli (STEC) are excreted in feces and persist in the environment until they die or recolonize a new host. Surface waters contaminated with manure-borne STEC can infect humans through drinking and recreational water use or irrigated crops that are minimally cooked. In this study, manure-impacted water microcosms mimicking beef cattle feedlot …

Fate And Transport Of Antibiotics And Antibiotic Resistance Genes In Runoff And Soil As Affected By The Timing Of Swine Manure Slurry Application, Renys Enrique Barrios, Himanshu Khuntia, Shannon L. Bartelt-Hunt, John E. Gilley, Amy M. Schmidt Schmidt, Daniel D. Snow, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Land application of swine manure slurry is a common practice to supplement nutrients to soil for crop production. This practice can introduce antibiotic residues and antibiotic resistance genes (ARGs) into the environment. Field testing is critical in identifying manure management practices effective in minimizing the environmental impacts of manure-borne antibiotic and ARGs. The objective of this study was to determine how the timing of swine manure application relative to rainfall events impacts the fate and transport of antibiotics and ARGs in surface runoff and manure-amended soil. Swine manure slurry was either broadcast or injected on test plots in the field. …

Occurrence And Persistence Of Antibiotics Administered To Cattle In A Newly Established Feedlot, Brittany Trejo

Department of Environmental Engineering: Theses and Student Research

The common practice of using therapeutic antibiotics in livestock farming is a worldwide phenomenon. Over the last decade, there has been a growing concern of antibiotics entering the environment via animal manure. Similar studies have focused on the occurrence and biological effects of antibiotics in land-applied animal feedlots; however, limited research has been conducted on the occurrence and persistence of antibiotics in animal feedlots. A study was conducted to investigate the occurrence and persistence of four injected antibiotics (ceftiofur enrofloxacin, florfenicol, and tulathromycin) and two continuously fed antibiotics (monensin and tylosin) in feedlot sediment, runoff, and sediment runoff. For antibiotics …

Narrow Grass Hedge Effects On Microbial Transport Following Variable Applications Of Beef Cattle Manure, Lisa M. Durso, John E. Gilley, Dave B. Marx, Brian L. Woodbury

Department of Biological Systems Engineering: Papers and Publications

The effectiveness of a 1.4 m wide grass hedge in reducing microbial transport following manure application was examined in this study. Beef cattle manure was applied to 0.75 m wide by 4.0 m long plots established on an Aksarben silty clay loam located in southeast Nebraska. Manure was added at rates required to meet none or the 1-, 2-, or 4-year nitrogen requirements for corn. The transport of phages, total coliforms, E. coli, and enterococci was measured for three 30 min simulated rainfall events, which were separated by approximately 24 h intervals. The narrow grass hedge reduced total counts of …

Rainwater Harvesting System Scenario Analysis On Runoff Reduction Potential In Surabaya, Indonesia: A Geospatial Analysis For Brantas Hilir Watershed, Putri Sukmahartati

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Global warming has become an environmental concern over the past several decades and its impact on the water cycle is very crucial to the well-being of the human population. In the hydrological cycle, water evaporates by the heat of the sun and atmosphere, where it is accumulated in the atmosphere via clouds and it then falls as rain. With warmer temperatures, more intensive evaporation and downpours occur. In addition, impervious surfaces are increasing as a result of urban development. Those surfaces cause more water to flow faster into open water bodies, creating more extensive flooding, and additionally reducing water quality. …

Impact Of Eastern Redcedar Proliferation On Water Resources In The Great Plains Usa—Current State Of Knowledge, Chris B. Zou, Dirac Twidwell, Christine H. Bielski, Dillon T. Fogarty, Aaron R. Mittelstet, Patrick J. Starks, Rodney E. Will, Yu Zhong, Bharat Sharma Acharya

Department of Biological Systems Engineering: Papers and Publications

In the Great Plains of the central United States, water resources for human and aquatic life rely primarily on surface runoff and local recharge from rangelands that are under rapid transformation to woodland by the encroachment of Eastern redcedar (redcedar; Juniperus virginiana) trees. In this synthesis, the current understanding and impact of redcedar encroachment on the water budget and water resources available for non-ecosystem use are reviewed. Existing studies concluded that the conversion from herbaceous-dominated rangeland to redcedar woodland increases precipitation loss to canopy interception and vegetation transpiration. The decrease of soil moisture, particularly for the subsurface soil layer, …

Setback Distance Requirements For Removal Of Swine Slurry Constituents In Runoff, John E. Gilley, Shannon L. Bartelt-Hunt, Kent M. Eskridge, Xu Li, Amy M. Schmidt, Daniel D. Snow

Department of Biological Systems Engineering: Papers and Publications

The use of setback distances for manure application on cropland areas adjacent to surface water bodies could serve a function similar to vegetative filter strips. However, little information currently exists to identify the setback distances necessary to effectively reduce the transport of contaminants in runoff. The objective of this study was to determine the effects of setback distance and runoff rate on concentrations of selected constituents in runoff following land application of swine slurry to a no-till cropland area in southeast Nebraska. The study site had a residue cover of 7.73 Mg ha-1 and a slope gradient of 4.9%. The …

Runoff Water Quality Characteristics Following Swine Slurry Application Under Broadcast And Injected Conditions, Nicole R. Schuster, Shannon L. Bartelt-Hunt, Lisa M. Durso, John E. Gilley, Xu Li, David B. Marx, Amy M. Schmidt, Daniel D. Snow

Department of Biological Systems Engineering: Papers and Publications

This study was conducted to measure the effects of swine slurry application method, time following slurry application, and runoff rate on selected water quality characteristics. Slurry from a commercial swine operation was broadcast or injected on field plots at a rate required to meet annual nitrogen requirements for corn. Rainfall simulation tests were conducted at five varying periods following slurry application. During each study period, three simulated rainfall events, separated by 24 h intervals, were applied for 30 min duration at an intensity of approximately 70 mm h^-1. Following the third rainfall simulation event, inflow was applied at …

Removal Of Cattle Manure Constituents In Runoff From No-Till Cropland As Affected By Setback Distance, John E. Gilley, Aaron J. Sindelar, Bryan L. Woodbury

Department of Biological Systems Engineering: Papers and Publications

Vegetative filter strips located at the bottom of a hillslope have been shown to substantially reduce nutrients and sediment in runoff. Cropland areas could serve a similar function. However, little scientifically derived information is available to help identify the setback distances required to effectively reduce the transport of contaminants in runoff. The objective of this study was to determine the effects of setback distance and runoff rate on the concentrations and mass transport rates of selected constituents following land application of beef cattle manure to a no-till cropland area. The study site had a residue cover of 8.84 Mg ha …

Removal Of Cattle Manure Constituents In Runoff From No-Till Cropland As Affected By Setback Distance, John E. Gilley, Aaron J. Sindelar, Brian L. Woodbury

Department of Biological Systems Engineering: Papers and Publications

Vegetative filter strips located at the bottom of a hillslope have been shown to substantially reduce nutrients and sediment in runoff. Cropland areas could serve a similar function. However, little scientifically derived information is available to help identify the setback distances required to effectively reduce the transport of contaminants in runoff. The objective of this study was to determine the effects of setback distance and runoff rate on the concentrations and mass transport rates of selected constituents following land application of beef cattle manure to a no-till cropland area. The study site had a residue cover of 8.84 Mg ha-1 …

Characterization Of Bridge Deck Runoff, Shannon L. Bartelt-Hunt, Elizabeth G. Jones, Renys Barrios

Nebraska Department of Transportation: Research Reports

In this study, time-weighted composite samples of bridge runoff and hourly traffic data were collected at four sampling locations in Nebraska. Total suspended solids (TSS) and hexane extractable material (HEM) had the highest concentrations during the first rainfall event of the year, while other constituent concentrations peaked during the third rainfall event of the year. In general, constituent concentrations were highest in the spring, and then decreased throughout the summer and fall. Average concentrations of TDS, TSS, chloride, iron, and lead measured in this study were higher than those found in the previous NDOR study at the same sampling locations. …

Infiltration And Runoff Parameters For Tilled And No-Till Row Crops, Andrew J. Volkmer

Department of Biological Systems Engineering: Dissertations and Theses

Infiltration and runoff are hydrologic processes that effect the amount of water available to plants, for groundwater recharge, and for stream flow. No-till planting (NT) is a management practice used to reduce soil erosion, increase water infiltration, and reduce soil water evaporation, and can have great impact on infiltration and runoff. An investigation was conducted to determine the impact of NT on infiltration and runoff when compared to tilled conditions.

Runoff and precipitation data was gathered from sites at Fillmore County, NE, Phelps County, NE, the USDA-ARS North Appalachian Experimental Watersheds (NAEW) near Coshocton, OH, and the Lennoxville Research Station …

Influence Of Grass Hedges On The Transport Of Antimicrobials, Antimicrobial Resistance Genes, And Antimicrobial Resistant Pathogens After Land Application Of Swine Manure, Shannon L. Bartelt-Hunt

Department of Civil and Environmental Engineering: Faculty Publications

The objective of this study was to determine the effects of narrow grass hedges on the fate and transport of antimicrobials and antimicrobial resistance genes (ARGs) in runoff and in soil following the land application of swine manure slurry. Swine manure slurry was land applied to 0.75m wide by 4.0m long plots established on an Aksarben silty clay loam soil located in southeast Nebraska. Swine manure was applied at a rate to meet the 3-year nitrogen (N) requirements for corn. Swine manure was applied to plots with and without a narrow grass hedge to evaluate the effect of the hedge …

Nutrient Concentrations Of Runoff As Affected By The Diameter Of Unconsolidated Material From Feedlot Surfaces, John E. Gilley, Gregory Boone, David B. Marx

Department of Biological Systems Engineering: Papers and Publications

Beef cattle feedlots contain unconsolidated material that accumulates on the feedlot surface during a feeding cycle. This study was conducted to measure the effects of varying diameters of unconsolidated surface material and varying flow rates on nutrient concentrations in runoff. Unconsolidated surface material with an average diameter of 4.76, 9.53, 19.1, or 47.5 mm and a composite sample with a 15.2 mm mean diameter were placed within 0.75 m wide × 4.0 m long plot areas. Flow was then introduced at the top of the plots in successive increments, and runoff samples for water quality analyses were obtained. Particle diameter …

Narrow Grass Hedge Effects On The Transport Of Antimicrobials And Antimicrobial Resistance Genes Following Land Application Of Swine Slurry, Bhavneet Soni

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

The objective of this study was to determine the effects of manure amendment and narrow grass hedges on the fate and transport of antimicrobials and ARGs in runoff and in soil following the land application of swine manure slurry. Swine manure slurry was land applied to 0.75m wide by 4.0m long plots established on an Aksarben silty clay loam soil located in southeast Nebraska. The treatment factor manure amendment consisted of two levels: no manure application and manure application to meet the 3 year nitrogen (N) requirements for corn. The treatment factor of grass hedge was established for half of …

Narrow Grass Hedge Effects On Nutrient Transport Following Swine Slurry Application, John E. Gilley, Shannon L. Bartelt-Hunt, Seth J. Lamb, Xu Li, David B. Marx, Daniel D. Snow, David B. Parker, Brian L. Woodbury

Department of Biological Systems Engineering: Papers and Publications

The effectiveness of a narrow grass hedge in reducing runoff nutrient loads following swine slurry application was examined. Slurry was applied to 0.75 m wide by 4.0 m long plots established on an Aksarben silty clay loam soil located in southeast Nebraska. Manure treatments consisted of no manure application and manure application to meet the 1, 2, or 3 year nitrogen (N) requirements for corn. Runoff water quality was measured during three 30 min simulated rainfall events. The grass hedge did not significantly reduce runoff nutrient transport after the swine slurry, which contained relatively small amounts of manure, was applied. …

Runoff Nutrient Transport As Affected By Land Application Method, Swine Growth Stage, And Runoff Rate, J. E. Gilley, Shannon L. Bartelt-Hunt, S. J. Lamb, X. Li, D. B. Marx, D. D. Snow, D. B. Parker, B. L. Woodbury

Department of Biological Systems Engineering: Papers and Publications

This study was conducted to measure the effects of slurry application method, swine growth stage, and flow rate on runoff nutrient transport. Swine slurry was obtained from production units containing grower pigs, finisher pigs, or sows and gilts. The swine slurry was applied using broadcast, disk, or injection methods at a rate required to meet annual nitrogen requirements for corn. Three 30 min simulated rainfall events, separated by 24 h intervals, were applied to the experimental plots at an intensity of 70 mm h^-1. Inflow was applied at the top of each plot in four successive increments after …

Characterization Of Runoff In Two Urban Watersheds In Lincoln, Nebraska, Michael J. Florek Iii

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Characterization of runoff water quality and conditions for two urban watersheds in Lincoln, NE, was performed using data collected from 2008-2012. Water quality data from in-stream probes and storm sampling was combined with soil sample data, geographic information system (GIS) modeling, and nutrient isotope composition analysis to provide a more detailed picture of runoff mechanisms in the Taylor Park and Colonial Hills watersheds.

Soil sample data and GIS modeling were used to compare physical characteristics of the urban watersheds. Taylor Park and Colonial Hills are relatively similar, save for Colonial Hills being six times as large. Its time of concentration, …

Wheat Strip Effects On Nutrient Loads Following Variable Manure Applications, Chance Thayer, John E. Gilley, Lisa M. Durso, David B. Marx

Department of Biological Systems Engineering: Papers and Publications

Vegetative filters have been found to significantly reduce nutrient loads in runoff. This study was conducted to: (1) evaluate the effects of a narrow wheat strip, varying manure application rates, and different overland flow rates on runoff nutrient loads following application of beef cattle manure; (2) determine the upper capacity of a narrow wheat strip to reduce nutrient loads by applying excessive amounts of manure; and (3) compare the effectiveness of narrow wheat strips and grass hedges in reducing runoff nutrient loads. A 1.4 m wide strip of actively growing winter wheat was located at the bottom of selected 0.75 …

Selecting Sprinkler Packages For Center Pivots, Derrel L. Martin, William L. Kranz, Allen L. Thompson, Hong Liang

Department of Biological Systems Engineering: Papers and Publications

Center pivots are the primary method of irrigation across the U.S. Great Plains. Center-pivot irrigation is also the fastest growing method of irrigation in the U.S. and around the world. Pivots have the potential to be very efficient and uniform if sprinkler devices are properly selected for local field conditions. New water application devices provide for selection that minimizes runoff and controls droplet sizes to reduce evaporation and drift losses. We present updates to models for computing runoff potential based on characteristics of sprinkler devices and soil textural classes. A dimensionless solution to the Green-Ampt infiltration method for center pivots …

Transport Of Trace Elements In Runoff From Unamended And Pond‐Ash Amended Feedlot Surfaces, Jason R. Vogel, John E. Gilley, Gary L. Cottrell, Brian L. Woodbury, Elaine D. Berry, Roger Eigenberg

Department of Biological Systems Engineering: Papers and Publications

The use of pond ash (fly ash that has been placed in evaporative ponds for storage and subsequently dewatered) for feedlot surfaces provides a drier environment for livestock and furnishes economic benefits. However, pond ash is known to have high concentrations of trace elements, and the runoff water‐quality effects of feedlot surfaces amended with pond ash are not well defined. For this study, two experimental units (plots) were established in eight feedlot pens. Four of the pens contained unamended soil surfaces, and the remaining four pens had pond‐ash amended surfaces. Before each test, unconsolidated surface material was removed from four …

Runoff, Erosion, And Size Distribution Of Sediment From Beef Cattle Feedlots, John E. Gilley, Jason R. Vogel, Roger Eigenberg, David B. Marx, Brian L. Woodbury

Department of Biological Systems Engineering: Papers and Publications

The size distribution of sediment in runoff from feedlot surfaces influences erosion rates and settling velocity. The objectives of this study were to: (1) measure runoff, erosion, and size distribution of sediment in runoff from feedlot surfaces containing varying amounts of unconsolidated surface material (USM), and (2) determine the effects of varying runoff rate on erosion and sediment size distribution. Simulated rainfall was applied to 0.75 m wide by 2 m long plots located within feedlot pens. Sieve and pipette analyses were used to measure the diameters of the eroded materials. No significant differences in runoff and erosion were found …

Improved Soil Mixing And Delivery System For A Storm Runoff Simulator, William C. Alms, Thomas G. Franti, David P. Shelton

Department of Biological Systems Engineering: Papers and Publications

An earlier version of a storm runoff simulator to test conservation buffers reproduced target hydrographs and sedigraphs using uniform, fine sand; however, it was unable to uniformly mix and deliver native sediment. The objectives of this work reported were to create a method to process native agricultural sediment, mix a uniform sediment slurry at a target concentration, and create a control system that will deliver the slurry in varying flow rates corresponding to a target sedigraph. Eroded silty clay (14% sand) was scraped, dried, and processed with a hammer mill. A sand (93% sand) and loam (44% sand) were dried …

Adsorption And Desorption Of Phosphorus And Nitrogen By Immersed Stalks, John E. Gilley, Bahman Eghball, David B. Marx

Department of Biological Systems Engineering: Papers and Publications

Adsorption and desorption of phosphorus (P) and nitrogen (N) by stalk residues may influence the concentrations of P and N in runoff. A laboratory study was conducted to measure the effects of P and N constituents in solution on adsorption and desorption of P and N by corn, soybean, and wheat stalk residues. Experimental variables included type of stalk material (corn, soybean, and wheat), inorganic nutrients in solution (PO₄-P and NO₃-N; PO₄-P and NH₄-N; NO₃-N and NH₄-N; and PO₄-P, NO₃-N, and NH₄-N), …

Narrow Grass Hedge Effects On Nutrient Transport Following Compost Application, John E. Gilley, Bahman Eghball, David B. Marx

Department of Biological Systems Engineering: Papers and Publications

The placement of stiff‐stemmed grass hedges on the contour along a hillslope has been shown to decrease nutrient transport in runoff. This study was conducted to measure the effectiveness of a narrow grass hedge in reducing runoff nutrient transport from plots with a range of soil nutrient values. Composted beef cattle manure was applied at dry weights of 0, 68, 105, 142, and 178 Mg ha^-1 to a silty clay loam soil and then incorporated by disking. Soil samples were collected 243 days later for analysis of water‐soluble phosphorus (WSP), Bray and Kurtz No. 1 phosphorus (Bray‐1 P), NO …

Spatial Variations In Nutrient And Microbial Transport From Feedlot Surfaces, John E. Gilley, Elaine D. Berry, Roger A. Eigenberg, David B. Marx, Bryan L. Woodbury

Department of Biological Systems Engineering: Papers and Publications

Nutrient and microbial transport by runoff may vary at different locations within a beef cattle feedlot. If the areas making the largest contributions to nutrient and microbial transport can be identified, it may be possible to institute site‐specific management practices to reduce runoff nutrient and microbial transport. The objectives of this study were to: (1) measure selected feedlot soil properties and nutrient and microbial transport in runoff from various feedlot locations, (2) compare the effects of unconsolidated surface materials (USM) (loose manure pack) and consolidated subsurface materials (CSM) (compacted manure and underlying layers) on nutrient and microbial transport, and (3) …

Nutrient Concentrations Of Runoff During The Year Following Manure Application, John E. Gilley, Bahman Eghball, D. B. Marx

Department of Biological Systems Engineering: Papers and Publications

Little information is currently available concerning temporal changes in nutrient transport following the addition of manure to cropland areas. This study was conducted to measure nutrient transport in runoff as affected by tillage and time following the application of beef cattle or swine manure to a site on which corn (Zea mays L.) was grown. Rainfall simulation tests were initiated 4, 32, 62, 123, and 354 days following land application. Three 30 min simulated rainfall events, separated by 24 h intervals, were conducted at an intensity of approximately 70 mm h-1. Dissolved phosphorus (DP), particulate phosphorus (PP), total phosphorus …

Nitrogen And Phosphorus Concentrations Of Runoff As Affected By Moldboard Plowing, John E. Gilley, Bahman Eghball, David B. Marx

Department of Biological Systems Engineering: Papers and Publications

The excessive application of manure on cropland areas can cause nutrients to accumulate near the soil surface. This study was conducted to measure the effects of moldboard plowing on the redistribution of nutrients within the soil profile and nutrient transport by overland flow. Composted beef cattle manure was applied at dry weights of 0, 68, 105, 142, and 178 Mg ha^-1 to a silty clay loam soil and then incorporated by disking. Selected plots were moldboard plowed 244 days later to a depth of approximately 23 cm. Soil samples for analysis of water‐soluble phosphorus, Bray and Kurtz No. 1 …

Water Erosion Prediction Project (Wepp): Development History, Model Capabilities, And Future Enhancements, Dennis C. Flanagan, John E. Gilley, Thomas G. Franti

Department of Biological Systems Engineering: Papers and Publications

The Water Erosion Prediction Project (WEPP) was initiated in August 1985 to develop new‐generation water erosion prediction technology for use by federal action agencies involved in soil and water conservation and environmental planning and assessment. Developed by the USDA‐ARS as a replacement for empirically based erosion prediction technologies, the WEPP model simulates many of the physical processes important in soil erosion, including infiltration, runoff, raindrop and flow detachment, sediment transport, deposition, plant growth, and residue decomposition. The WEPP project included an extensive field experimental program conducted on cropland, rangeland, and disturbed forest sites to obtain data required to parameterize and …