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Numerical Investigation Of Cryopreserved Zebrafish Sperm Cell Activation In Microchannels, Thomas Foster Scherr
Numerical Investigation Of Cryopreserved Zebrafish Sperm Cell Activation In Microchannels, Thomas Foster Scherr
LSU Doctoral Dissertations
This aim of this research project is to probe the activation process of zebrafish spermatozoa. Zebrafish are a model species for biological engineering applications, and the cryopreservation of their reproductive cells allows for inexpensive cataloging and maintenance of valuable biological material. Evaluation of cryopreservation protocols for aquatic sperm cells is typically accomplished by motility analysis after subjecting cells to a cryopreservation treatment. In zebrafish sperm cells, motility is initiated when cells come into contact with a hypo-osmotic environment. Subsequent activation analysis is currently done manually and brings with it an inherent difficulty and error. This process is slow and not …
Applications Of Cfd Simulations On Studying The Multiphase Flow In Microfluidic Devices, Yuehao Li
Applications Of Cfd Simulations On Studying The Multiphase Flow In Microfluidic Devices, Yuehao Li
LSU Doctoral Dissertations
Microfluidics has been extensively investigated as a unique platform to synthesize nanoparticles with desired properties, e.g., size and morphology. Compared to the conventional batch reactors, wet-chemical synthesis using continuous flow microfluidics provides better control over addition of reagents, heat and mass transfer, and reproducibility. Recently, millifluidics has emerged as an alternative since it offers similar control as microfluidics. With its dimensions scaled up to millimeter size, millifluidics saves fabrication efforts and potentially paves the way for industrial applications. Good designs and manipulations of microfluidic and millifluidic devices rely on solid understanding of fluid dynamics. Fluid flow plays an important role …