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Biological and Agricultural Engineering Undergraduate Honors Theses

Theses/Dissertations

2015

Biomass

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A Study On The Be2100 Noninvasive Biomass Sensor As An Instrument For Measuring Optical Density During Fed-Batch Fermentation At Various Stir Rates, Hannah Moreland May 2015

A Study On The Be2100 Noninvasive Biomass Sensor As An Instrument For Measuring Optical Density During Fed-Batch Fermentation At Various Stir Rates, Hannah Moreland

Biological and Agricultural Engineering Undergraduate Honors Theses

Fusion proteins, engineered proteins that combine the DNA sequences and therefore properties of two different proteins, can be used in a variety of therapeutic purposes. One example of a therapeutic fusion protein is collagen binding domain-parathyroid hormone glutathione-S-transferase (CBD-PTH-GST), which can combat osteoporosis by binding specifically to collagen in the vertebral column and promoting bone growth through the release of calcium. This fusion protein is being expressed with Escherichia coli at the University of Arkansas through fed-batch fermentation, a method that produces large volume of cells through fermentation in a controlled environment in a bioreactor. It is necessary to track …