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Survey Of The Great Kanawha River, West Virginia, For Virulence Related Gene Markers Stx1, Stx2, And Eaea, Christina Carole Johnson
Survey Of The Great Kanawha River, West Virginia, For Virulence Related Gene Markers Stx1, Stx2, And Eaea, Christina Carole Johnson
Theses, Dissertations and Capstones
Microbial surveying using antibiotic resistant bacteria, fecal coliforms, and virulence genes is an approach not previously tested on the Great Kanawha River. Research objectives were to test antibiotic resistant and fecal coliform bacteria as bioindicators of water quality, and develop a multiplex- polymerase chain reaction (mPCR) system for identification of stx1, stx2, and eaeA DNA sequences in isolated fecal coliforms (FC). Fecal indicator populations and antibiotic resistant populations were demonstrated to be independent. Bacterial populations were assigned impact score(s) (IS) values for each sample site based on data set percent ranks. Population scores were summed per sample site to generate …