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Highly Divergent 16s Rrna Sequences In Ribosomal Operons Of Scytonema Hyalinum (Cyanobacteria), Jeffrey R. Johansen, Jan Mares, Nicole Pietrasiak, Marketa Bohunicka, Jan Zima Jr., Lenka Stenclova, Tomas Hauer Oct 2017

Highly Divergent 16s Rrna Sequences In Ribosomal Operons Of Scytonema Hyalinum (Cyanobacteria), Jeffrey R. Johansen, Jan Mares, Nicole Pietrasiak, Marketa Bohunicka, Jan Zima Jr., Lenka Stenclova, Tomas Hauer

2017 Faculty Bibliography

A highly divergent 16S rRNA gene was found in one of the five ribosomal operons present in a species complex currently circumscribed as Scytonema hyalinum (Nostocales, Cyanobacteria) using clone libraries. If 16S rRNA sequence macroheterogeneity among ribosomal operons due to insertions, deletions or truncation is excluded, the sequence heterogeneity observed in S. hyalinum was the highest observed in any prokaryotic species thus far (7.3± 9.0%). The secondary structure of the 16S rRNA molecules encoded by the two divergent operons was nearly identical, indicating possible functionality. The 23S rRNA gene was examined for a few strains in this complex, and it …


Chemical Characterization Of The Adhesive Secretions Of The Salamander Plethodon Shermani (Caudata, Plethodontidae), Janek Von Byern, Ingo Grunwald, Max Kosok, Ralph Saporito, Ursula Dicke, Oliver Wetjen, Karsten Thiel, Kai Borcherding, Thomas Kowalik, Martina Marchetti-Deschmann Jul 2017

Chemical Characterization Of The Adhesive Secretions Of The Salamander Plethodon Shermani (Caudata, Plethodontidae), Janek Von Byern, Ingo Grunwald, Max Kosok, Ralph Saporito, Ursula Dicke, Oliver Wetjen, Karsten Thiel, Kai Borcherding, Thomas Kowalik, Martina Marchetti-Deschmann

2017 Faculty Bibliography

Salamanders have developed a wide variety of antipredator mechanisms, including tail autotomy, colour patterns, and noxious skin secretions. As an addition to these tactics, the red-legged salamander (Plethodon shermani) uses adhesive secretions as part of its defensive strategy. The high bonding strength, the fast-curing nature, and the composition of the biobased materials makes salamander adhesives interesting for practical applications in the medical sector. To understand the adhesive secretions of P. shermani, its components were chemically analysed by energy dispersive X-ray spectroscopy (EDX), inductively coupled plasma mass spectrometry (ICP-MS), amino acid analysis, and spectroscopy (ATR-IR, Raman). In addition, proteins were separated …


Variable Alkaloid Defenses In The Dendrobatid Poison Frog Oophaga Pumilio Are Perceived As Differences In Palatability To Arthropods, Sarah Bolton, Kelsey Dickerson, Ralph Saporito Mar 2017

Variable Alkaloid Defenses In The Dendrobatid Poison Frog Oophaga Pumilio Are Perceived As Differences In Palatability To Arthropods, Sarah Bolton, Kelsey Dickerson, Ralph Saporito

2017 Faculty Bibliography

Conspicuously colored dendrobatid frogs sequester alkaloid defenses from dietary arthropods, resulting in considerable alkaloid variation among populations; however, little is known about how variation is perceived as a defense against predators. Previous studies have found variable alkaloids in the dendrobatid Oophaga pumilio to be associated with differences in toxicity to laboratory mice, suggesting variable defenses are important. Arthropods are natural predators that use chemoreception to detect prey, including frogs, and may therefore perceive variation in alkaloid profiles as differences in palatability. The goal of the present study is to determine how arthropods respond to variable alkaloid defenses in O. pumilio. …


Escape Behaviour Of Aposematic (Oophaga Pumilio) And Cryptic (Craugastor Sp.) Frogs In Response To Simulated Predator Approach, Ralph Saporito Mar 2017

Escape Behaviour Of Aposematic (Oophaga Pumilio) And Cryptic (Craugastor Sp.) Frogs In Response To Simulated Predator Approach, Ralph Saporito

2017 Faculty Bibliography

Crypsis and aposematism are common antipredator strategies that can each be coupled with behaviours that maximize predator deterrence or avoidance. Cryptic animals employ camouflage to conceal themselves within their environment and generally rely on immobility to avoid detection by predators. Alternatively, aposematic animals tend to rely on an association between conspicuous colouration and secondary defence to deter potential predators, and tend to exhibit slow movements in response to predators. The goal of the present study was to determine how cryptic Craugastorsp. and aposematic Oophaga pumilio respond to simulated human and bird model predators. Oophaga pumilio responded more often with movement …


Deimatic Behaviour Exhibited By The Green And Black Poison Frog (Dendrobates Auratus) After Exposure From A Cover Object, Ralph Saporito Jan 2017

Deimatic Behaviour Exhibited By The Green And Black Poison Frog (Dendrobates Auratus) After Exposure From A Cover Object, Ralph Saporito

2017 Faculty Bibliography

No abstract provided.


Cyanomargarita Gen. Nov. (Nostocales, Cyanobacteria): Convergent Evolution Resulting In, Jeffrey R. Johansen, Sergei Shalygin, Regina Shalygina, Nicole Pietrasiak Jan 2017

Cyanomargarita Gen. Nov. (Nostocales, Cyanobacteria): Convergent Evolution Resulting In, Jeffrey R. Johansen, Sergei Shalygin, Regina Shalygina, Nicole Pietrasiak

2017 Faculty Bibliography

Two populations of Rivularia‐like cyanobacteria were isolated from ecologically distinct and biogeographically distant sites. One population was from an unpolluted stream in the Kola Peninsula of Russia, whereas the other was from a wet wall in the Grand Staircase‐Escalante National Monument, a desert park‐land in Utah. Though both were virtually indistinguishable from Rivularia in field and cultured material, they were both phylogenetically distant from Rivularia and the Rivulariaceae based on both 16S rRNA and rbcLX phylogenies. We here name the new cryptic genus Cyanomargarita gen. nov., with type species C. melechinii sp. nov., and additional species C. calcarea …


A Revision Of The Genus Geitlerinema And A Description Of The Genus Anagnostidinema Gen. Nov. (Oscillatoriophycidae, Cyanobacteria), Jeffrey R. Johansen, Otakar Strunecký, Markéta Bohunická, Kateřina Čapková Čapková, Lenka Raabová, Petr Dvořák, Jiří Komárek Jan 2017

A Revision Of The Genus Geitlerinema And A Description Of The Genus Anagnostidinema Gen. Nov. (Oscillatoriophycidae, Cyanobacteria), Jeffrey R. Johansen, Otakar Strunecký, Markéta Bohunická, Kateřina Čapková Čapková, Lenka Raabová, Petr Dvořák, Jiří Komárek

2017 Faculty Bibliography

The simple filamentous cyanobacterial genus Geitlerinema is heterogeneous. At least two distinct phylogenetic clades can be derived from the set of most common freshwater Geitlerinema species. Our revision is based on the original description of the type species G. splendidum aka Oscillatoria spendida and on molecular sequencing of morphologically relevant strains. The revised Geitlerinema contains only one species according to morphological similarity with its original description. Consequently, the majority of other freshwater species inferred from molecular sequencing of 16S rRNA gene and 16S–23S ITS analysis (related both genetically and morphologically to G. pseudacutissimum) must be classified as a special taxon …


Komarekiella Atlantica Gen. Et Sp. Nov. (Nostocaceae, Cyanobacteria): A New Subaerial Taxon From The Atlantic Rainforest And Kauai, Hawaii, Jeffrey R. Johansen, Guilherme Scotta Hentschke, Nicole Pietrasiak, Janaina Rigonato, Marli F. Fiore, Célia Leite Sant’Anna Jan 2017

Komarekiella Atlantica Gen. Et Sp. Nov. (Nostocaceae, Cyanobacteria): A New Subaerial Taxon From The Atlantic Rainforest And Kauai, Hawaii, Jeffrey R. Johansen, Guilherme Scotta Hentschke, Nicole Pietrasiak, Janaina Rigonato, Marli F. Fiore, Célia Leite Sant’Anna

2017 Faculty Bibliography

Six strains of Cyanobacteria sampled in the Brazilian Atlantic rainforest and one strain from Kauai, Hawaii, were studied using morphological and molecular approaches, including 16S rRNA gene phylogenies and 16S–23S ITS secondary structures, and are herein described as Komarekilla atlantica gen. et sp. nov.. Morphologically they are similar to Nostoc, Desmonostoc, Halotia, and Mojavia and indistinguishable from Chlorogloeopsis. The parsimony and Bayesian phylogenies of the 16S rDNA show that these strains are close to nostocacean strains, in strongly supported clades and separated from all other genera. The secondary structures of the 16S–23S ITS were very consistent between strains of K. …