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Ability Of Skin Bacteria On The Panamanian Frog Species, Craugastor Fitzingeri, To Inhibit The Fungal Pathogen Batrachochytrium Dendrobatidis, Tiffany N. Bridges Dec 2015

Ability Of Skin Bacteria On The Panamanian Frog Species, Craugastor Fitzingeri, To Inhibit The Fungal Pathogen Batrachochytrium Dendrobatidis, Tiffany N. Bridges

Senior Honors Projects, 2010-2019

An emerging infectious disease caused by the fungus Batrachochytrium dendrobatidis (Bd) is leading to global amphibian declines and is threatening the biodiversity of amphibians. Bd susceptibility varies among individuals, species, and populations perhaps due to defensive mechanisms such as symbiotic skin microbes. Some species of amphibians such as Craugastor fitzingeri, a terrestrial frog native to Central America, continue to persist in Bd-positive environments in Panama. My study focused on identifying antifungal bacterial isolates and determining the culturability of the bacterial community on 15 individuals of C. fitzingeri. Morphologically distinct isolates were challenged against Bd in …


Insomniac Of The Soil: A Collection Of Poetry And Essays, Sarah E. Golibart May 2015

Insomniac Of The Soil: A Collection Of Poetry And Essays, Sarah E. Golibart

Senior Honors Projects, 2010-2019

“Insomniac of the Soil” is a homage to a landscape that has deeply informed Sarah Golibart's life and her artistic voice – the tidewater flatlands of Virginia’s Chesapeake Bay peninsula where her family lives and where Golibart has worked on farms since high school. Both her poems and essays are earthy, imagistic, and grounded – quite literally – in the soil as well as in a sensibility of ecological ethics and sustainability. “Insomniac of the Soil” is also a love song to the fervent and fallow cycles of the soil.


Comparative Analysis Of Anti-Bd Bacteria From Six Malagasy Frog Species Of Ranomafana National Park, Kelsey Savage May 2015

Comparative Analysis Of Anti-Bd Bacteria From Six Malagasy Frog Species Of Ranomafana National Park, Kelsey Savage

Senior Honors Projects, 2010-2019

As Malagasy amphibians are facing an impending extinction crisis from the lethal skin fungus Batrachochytrium dendrobatidis (Bd), it has become imperative to proactively mitigate the threat. Bd sporangia develop in the skin of infected amphibians and cause the skin to thicken, leading to ionic imbalance and eventual heart failure. It has been shown that certain bacterial species are able to inhibit Bd growth on amphibians by producing antifungal metabolites. Community-based probiotics are one approach used to combat chytridomycosis by inoculating an environment with Bd-inhibitory bacteria so that many amphibian species are treated at once. With this method, it is important …


Experimental Greenhouse And Field Trials On American Ginseng, Panax Quinquefolium: Implications For Restoration In Appalachia, Emily Thyroff May 2015

Experimental Greenhouse And Field Trials On American Ginseng, Panax Quinquefolium: Implications For Restoration In Appalachia, Emily Thyroff

Senior Honors Projects, 2010-2019

Panax quinquefolium, American ginseng, is one of the more valuable non-timber forest products, NTFPs, providing economic, cultural, and ecological ecosystem services in forests. Although ginseng has a broad distribution range, it is not abundant anywhere due to overharvesting and deer browse. This study included experimental field and greenhouse trials to determine optimal growing conditions given inconsistencies regarding aspect and soil. Three soil series and two aspects (represented by soil moisture in the greenhouse) were manipulated in a factorial design. We hypothesized that there would be significant differences in ginseng performance (germination, survival, leaf area, and height) due to soil and …


Comparing Pseudo-Environmental And Horizontal Plus Pseudo-Environmental Transmission Of A Probiotic Janithinobacterium Lividum Between Green Frog Tadpoles, Stephen Simonetti May 2015

Comparing Pseudo-Environmental And Horizontal Plus Pseudo-Environmental Transmission Of A Probiotic Janithinobacterium Lividum Between Green Frog Tadpoles, Stephen Simonetti

Senior Honors Projects, 2010-2019

The gram-negative bacteria, Janthinobacterium lividum (J.liv) is able to kill the pathogenic fungus known as Batrachochytrium dendrobatidis (Bd), which is known to be causing massive amphibian decline worldwide. The purpose of this research was to identify the ability of the probiotic bacteria, Janthinobacterium lividum (J.liv), to be horizontally transmitted from individual to individual and/or pseudo-environmentally transmitted from individual to environment and back to individual between Lithobates clamitans (green frog) tadpoles. Based on previous findings I hypothesized that pseudo-environmental and horizontal transmission will occur in tadpoles. This would be important for helping stop the decline of amphibians because it would provide …


Using Capture-Mark-Recapture Techniques To Estimate Detection Probabilities & Fidelity Of Expression For The Critically Endangered James Spinymussel (Pleurobema Collina)., Alaina C. Esposito May 2015

Using Capture-Mark-Recapture Techniques To Estimate Detection Probabilities & Fidelity Of Expression For The Critically Endangered James Spinymussel (Pleurobema Collina)., Alaina C. Esposito

Masters Theses, 2010-2019

The critically endangered James Spinymussel (Pleurobema collina) is a species of freshwater mussel endemic to Virginia’s James and Dan River basins. In the last 20 years, P. collina has experienced a substantial decline in numbers and currently occupies approximately 10% of its original habitat; however, little information is known about this species to assist in conservation. A 230-meter reach of transitional habitat in Swift Run was selected for repeat observations to estimate detection probabilities using a Capture-Mark-Recapture framework. In June 2014, visual scouting began to locate and tag P. collina (including other mussels in the community) with PIT …


The Effect Of Controlled Burns On Abundance Of Woody Species At Buck Mountain, West Virginia, Barry E. Edgar May 2015

The Effect Of Controlled Burns On Abundance Of Woody Species At Buck Mountain, West Virginia, Barry E. Edgar

Masters Theses, 2010-2019

Each year, the U.S. Forest Service prescribes burns within the George Washington and Jefferson National Forest (GWJNF). Burns are prescribed in the growing (late April-October) and dormant season (November- mid-April). The goal of the burns is to reinstate the natural fire regime, returning forests to their original species composition. Currently in GWJNF, Appalachian pine-oak forests are experiencing an increase in fire-intolerant species, while Quercus species and Gaylussacia brachycera, an endangered shrub species, are declining. In the summer of 2014, a vegetation survey was conducted on Buck Mountain, West Virginia to determine if there was a significant difference between dormant and …