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Articles 1 - 6 of 6
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Are Mussel Shells Environmental Dna Time Capsules? A Comparison Of Extraction Methods For Obtaining Dna From Shell Material., Kelsey Elizabeth Rogers
Are Mussel Shells Environmental Dna Time Capsules? A Comparison Of Extraction Methods For Obtaining Dna From Shell Material., Kelsey Elizabeth Rogers
Online Theses and Dissertations
Freshwater mussels have become some of the most imperiled species in North America and widespread populations have succumbed to pollution and many other anthropogenic-related factors. With molecular techniques evolving, a recent interest in ancient DNA and museum specimens has emerged and prompted a study to test the ability of several extraction methods to isolate DNA from museum mussel specimens. The purpose of this study was to determine if four DNA extraction methods had influence on total DNA yield (ng/mg) from mussel tissue. The hinge ligaments of freshwater mussels ranging in collection date (1984-2015) were used as the source of genetic …
Comparison Of Cytochrome B Variation And Morphological Features Of Isolated Banded Pygmy Sunfish (Elassoma Zonatum) Populations In The Lower Green River And Coastal Plain Systems In Western Kentucky, David Cravens
Online Theses and Dissertations
The banded pygmy sunfish Elassoma zonatum is a wide-ranging species found throughout the Southeastern Coastal Plain of North America. In Kentucky, the populations are found above and below the inner boundary of the Coastal Plain. Due to their geographical separation comparisons in body size and morphological features along with genetic comparisons using mitochondrial cytochrome-b (Cyt-b) were conducted to test relationships between populations. Two sites, Cypress Creek in the Lower Green River system and Rose Creek in the Tradewater River drainage, were sampled from above the inner boundary and two additional sites, Bayou de Chien and Running Slough in the Mississippi …
Habitat Use By Wintering Passerines In Fire-Managed Forests Along The U.S. Gulf Coast, Justin Michael Michaud
Habitat Use By Wintering Passerines In Fire-Managed Forests Along The U.S. Gulf Coast, Justin Michael Michaud
Online Theses and Dissertations
Coastal habitats are being impacted by land development, fragmentation, and disturbance related to climate change. The remaining natural areas need to use planned management that may, in some cases, include the use of prescribed fire to maintain habitat quality. Numerous species of passerines, including some with declining populations, use the Gulf Coast as a wintering area, and some depend on habitats managed by fire. To provide information for land managers, I studied the winter bird community at Naval Live Oaks in Gulf Islands National Seashore with two primary objectives: (1) to describe the distribution, abundance, and diversity of the non-breeding …
Natural Environmental Gradients Predict The Microhabitat Use, Fine-Scale Distribution, And Abundance Of Three Woodland Salamanders In An Old-Growth Forest, Joseph Alex Baecher
Natural Environmental Gradients Predict The Microhabitat Use, Fine-Scale Distribution, And Abundance Of Three Woodland Salamanders In An Old-Growth Forest, Joseph Alex Baecher
Online Theses and Dissertations
Woodland salamanders (Plethodonidae: Plethodon)－a group of sensitive, direct developing, lungless amphibians－are particularly responsive to gradients in environmental conditions. Because of their functional dominance in terrestrial ecosystems, woodland salamanders are responsible for the transformation of nutrients and translocation of energy between highly desperate levels of trophic organization (detrital food webs and high‐order predators). However, the spatial extent of woodland salamanders' role in the ecosystem is likely contingent upon the distribution of their biomass throughout the forest. Therefore, a better understanding of woodland salamander spatial population dynamics is needed to further understand their role in terrestrial ecosystems. The objectives of this study …
Sample Collection And Dna Extraction Methods For Environmental Dna Metabarcoding In Headwater Streams, Michael Triston Mullins
Sample Collection And Dna Extraction Methods For Environmental Dna Metabarcoding In Headwater Streams, Michael Triston Mullins
Online Theses and Dissertations
DNA is found to be free and ubiquitous in the environment where it is no longer associated with the source organism, and is also known as environmental-DNA (eDNA). Methods optimized for specific environments may be able supplement insight to local taxa richness. With the advent of high throughput sequencing and the proliferation of sequence data in public repositories, insights to the biodiversity of communities at the molecular level have been possible. Thus, this study compared commonly used DNA capture (water precipitation and filtration) and extraction (MoBio's PowerWater, Qiagen's DNeasy Blood and Tissue Kit, and a CTAB protocol) methods in their …
Assessment Of The Macroinvertebrate Assemblages From The Mesohabitats Of A Headwater Stream-Wetland Hydrologic Restoration In Eastern Kentucky, Rebecca Jo Roberts
Assessment Of The Macroinvertebrate Assemblages From The Mesohabitats Of A Headwater Stream-Wetland Hydrologic Restoration In Eastern Kentucky, Rebecca Jo Roberts
Online Theses and Dissertations
The bulk of streams in the U.S. have been negatively impacted by anthropogenic disturbances and the streams of Kentucky are no exception. In recent decades stream restoration has become a common practice in order to improve habitat degradation resulting from land use practices such as channelization. Despite the large amount of effort and funding stream restoration projects represent, only a small portion have undergone post-restoration assessments of the ecological response in the restored streams. Slabcamp Creek, a headwater stream located in the Licking River basin in eastern Kentucky, underwent a stream-wetland hydrologic restoration in 2010 in order to improve hydrologic …