Digital Commons Network^™

Biodegradation Of Methyl Tert-Butyl Ether And Tert-Butyl Alcohol Using Bioaugmentation With Biowish® Aqua, Elizabeth Villanueva

Master's Theses

Aqua, a commercial product manufactured by BiOWiSH® Technologies, was utilized in this research to study its effectiveness to biodegrade methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA). Microcosms containing varying concentrations of MTBE and TBA as well as a growth media and mineral salt solution were examined. Analytical instrumentation used in this study included the use of a gas chromatograph-mass spectrometer (GC/MS) to determine concentrations of MTBE and TBA and a spectrophotometer to extrapolate approximate active biomass concentrations in each experiment. Four different environmental conditions were tested for both MTBE and TBA. The environmental conditions tested for each contaminant included: …

Application Of A 14c-Assay To Assess Methanotrophic Biodegradation Of Tce In Low Ph Groundwater, Evan Groome

All Theses

Current biological strategies for remediating trichloroethylene (TCE) in low pH aquifers (i.e., pH14C-TCE assay was developed to determine pseudo first-order rate constants for the degradation of TCE in microcosms containing soil and groundwater from the Boeing Michigan Aeronautical Research Center (BOMARC) superfund site, where the pH ranges from 4.1 to 4.9. The ¹⁴C-TCE assay was also adapted to calculate soil-normalized rate constants for data from this site, as well as data that Szwast²¹ collected from the Beltsville Agricultural Research Center (BARC). In addition to natural attenuation, biostimulation through amendments of methane and nutrients were also assessed. This treatment …

Biodegradation Of Rubber Particles In Soil: Using Acclimated Bacteria Isolated From Kansas Soil To Degrade Cryogrinds In Slurry, Shane Graham

Williams Honors College, Honors Research Projects

This study investigated the viability of bioremediating rubber cryogrind using enriched indigenous bacteria. To begin the experiment, soils from three highway roadside locations in Kansas, KS 96 and West, KS 400 and 143^rd, and 199^th, were collected and transported to the lab to be studied. An initial soil characterization was run on the soil samples using distilled (DI) water mixture and 0.01 M CaCl₂ to assess conductivity. The soils were tested to gather a baseline of the relationship between pH and conductivity and the impact of its distance from the roadside. Bacteria were isolated from …

Composting Processes For Food Processing Wastes: A Review, Yung-Tse Hung, Kevin P. Holloman, Howard Paul, Christopher Huhnke

Civil and Environmental Engineering Faculty Publications

Composting of food processing waste was analyzed as a biological process and an engineered system. The goal is to establish fundamental principles and design criteria that would aid its adoption as waste management practice. Characteristics of the inflow, reactor, and outflow were evaluated. Success of the bioreactor was found to be largely dependent on microbial community structure, physical properties of biodegradable waste (BW), aeration, heat transfer, and time required for maturation. Static piles were the primary focus of this article for cost and energy efficiency.

Treatment Of Perchlorate And Multiple Oxyanions: Chemical And Biological Investigations, Mark Elkouz

UNLV Theses, Dissertations, Professional Papers, and Capstones

Groundwater contamination with oxyanions is an issue that must be addressed for environmental, ecological and societal reasons. Chemical and biological treatment methods of reduction are already known and practiced, but the simultaneous presence of multiple oxyanions can add complicating effects to treatment sequence and efficacy. This research was concerned with the investigation of the chemical reductive treatment of Cr(VI) using both calcium polysulfide (CaSx) and ferrous sulfate (FeSO4), and the biological reductive treatment of chlorate, Cr(VI), nitrate and perchlorate using multiple organic substrates. The chemical treatment phase consisted of initial jar tests, using CaSx and FeSO4, followed by a laboratory …

Methods To Improve The Remediation Of Polycyclic Aromatic Hydrocarbons (Pahs) In Aerobic And Anaerobic Environments, Brian Wartell

Dissertations

Oil spills occur regularly in terrestrial environments and crude oil can contain many compounds that are highly resistant to degradation. Among these compounds are high levels of polycyclic aromatic hydrocarbons (PAHs) which are not only toxic but can also be carcinogenic and/or mutagenic. The first chapter of this dissertation includes an extensive review chapter on the variables affecting the anaerobic degradation of hydrocarbons, with a particular focus on PAHs. Electron acceptors, electron donors, temperature, salinity, pH all play key roles in determining the possibility effective of effective degradation occurring. Thus, by addressing solutions, such as biostimulation, improving environmental variables for …

Enhancing The Performance Of Anaerobic Bioreactors For The Biological Degradation Of Volatiles Organic Compounds In Groundwater Using Alternate Electron Acceptors, Leslie Michele Pipkin

LSU Doctoral Dissertations

Engineered anaerobic bioreactors (ABR) constructed from mixed media and sand can biodegrade high concentrations of volatile organic compounds (VOCs). Sustainable treatment of benzene, toluene, ethylbenzene, and xylenes (BTEX), chlorinated ethenes and ethanes were used to investigate the use of engineered ABRs, while also analyzing the system performance of a full-scale ABR system. Experimentations conducted on environmental and biological factors that affect ABR system performance was conducted through serum bottles, columns, and reactor studies.

Column and reactor ABR systems were designed to treat VOCs via biodegradation in groundwater with varying terminal electron acceptors (TEAs). Laboratory-scale column ABRs were constructed to treat …

Evaluation Of Disinfection Byproduct Speciation Models Based On Biodegradation And Chemical Decomposition, Xian Ma

Environmental & Water Resources Engineering Masters Projects

The USEPA has regulated both chlorinated and brominated trihalomethanes (THMs) and haloacetic acids (HAAs) since Stage 2 D/DBP Rule. Among regulated species, all four chlorinated and brominated THMs are regulated as total TTHMs (TTHM). But of the nine HAAs, only the sum of five (HAA5) are regulated, with no detail on individual species. This leaves four unregulated HAAs of which all contain bromine. With more attention on the brominated haloacetic acids due to their higher toxicity, the focus on the formation of those unregulated brominated species is elevated. The objective of this study is to assess national occurrence of all …

Evaluation Of Electron Donors For Perchlorate And Nitrate Biodegradation In Contaminated Groundwater Applications, Mayra Alejandra Sarria Cortes

UNLV Theses, Dissertations, Professional Papers, and Capstones

In the United States, perchlorate contamination has been widely reported, including in Las Vegas, Nevada, where perchlorate has been detected at concentrations of 34.7 mg/kg in vadose zone soil and 0.18-3.7 g/L in groundwater. Once this groundwater reaches the Las Vegas Wash, there is potential for widespread contamination of drinking water sources throughout the Southwest, including in Nevada, Arizona, and California. This issue is becoming increasingly important because even at low perchlorate concentrations, sensitive populations such as infants and pregnant women can be potentially impacted due to perchlorate’s ability to hinder iodine uptake into the thyroid glands, which leads to …

Fate Of Benzalkonium Chlorides In Natural Environment And Treatment Processes, Adnan Hossain Khan

Electronic Thesis and Dissertation Repository

Benzalkonium chlorides (BACs) are a type of cationic surfactant and are highly adsorptive to negatively charged surfaces during the wastewater treatment process. They can, therefore, enter the aquatic environment via the suspended organic matter in wastewater effluents, and the terrestrial environment through the application of biosolids as a soil amendment for crop production or by the use of reclaimed wastewater for irrigation. This research investigated the fate of the two most commonly used BACs, benzyl dimethyl dodecyl ammonium chloride (BDDA; C₁₂-alkyl chain) and benzyl dimethyl tetradecyl ammonium chloride (BDTA; C₁₄-alkyl chain), individually and in mixture in …

Sustainable Decolorization Of Reactive And Acid Dye Wastewater Using Photo-Fenton Oxidation Both With And Without Biodegradation: Laboratory And Field Studies, Marissa R. Jablonski

Theses and Dissertations

Photo-Fenton oxidation is an advanced oxidation process (AOP) used to degrade low-concentration textile dye wastewater using expensive chemicals. The technique has shown promise in laboratory-scale projects, but has not been scaled up sustainably to function for industry. Aerobic biodegradation is a common biological treatment method used in large-scale textile industrial applications that generates large amounts of hazardous biological waste. This waste is often left open to the elements and subsequently leaches into natural waterways or onto land. This is the first study of its kind to combine the two to treat cottage-scale industry-grade textile wastewater with a dye concentration of …

Modeling Biodegradation Settlement Of Municipal Solid Waste (Msw) Based On Measurement Of Landfill Gas And Degradable Solids In Leachate Recirculated Bioreactors, Vatsal Atulkumar Shah

Dissertations

The purpose of this work is two-fold: 1) to understand the phenomenon of the biodegradation process of municipal solid waste (MSW) in leachate recirculated bioreactors, and 2) to create a realistic predictive model based on this understanding which is capable of supporting a laboratory-to-field relationship for bioreactor landfills. Biodegradation is best described by loss of mass; however, primary researchers have assumed the phenomenon to be purely volume loss and modeled best by mechanical processes using a conservation of energy approach. It is suggested that the phenomenon requires a fundamental understanding of biodegradation process which results in a loss of mass, …

The Occurrence, Fate, Environmental Impact, And Management Implications Of Pharmaceutical And Personal Care Products In Wastewater And The Environment, Benjamin Donald Blair

Theses and Dissertations

Pharmaceuticals and personal care products (PPCPs) are a critical part of modern life. However, there is growing evidence that the levels of PPCPs detected in wastewater effluent and in the environment have the potential to cause damage to aquatic organisms. These pollutants enter the aquatic environment primarily through human use, disposal in the drain or toilet, land application of biosolids, and veterinary sources. Numerous questions remain regarding the occurrence, fate, and impacts of PPCPs in wastewater, along with limited feasible management recommendations that would adequately mitigate the risk from these pollutants. This dissertation will present three advances in the field …

Biogeochemical Controls On Fate Of Subsurface Oiled Sands On A Coastal Headland Beach, Autumn Anastasia Westrick

LSU Master's Theses

The fate of subsurface oiled sands collected from Fourchon Beach in Louisiana were determined while modifying biogeochemical controls on the degradation of PAHs. Groundwater on the beach has intrinsically low dissolved oxygen concentrations, which may limit natural biodegradation of the crude oil components. The intent of this research was to characterize the biogeochemical properties and degradability of oiled sands (with >10% of pore filled with MC252 oil) using a combination of laboratory flow-through reactor studies, field measurements and time-series microelectrode profiles of down-flow and cross flow geometries. Reactor experiments indicate that optimal conditions for substantial oil degradation are aerobic under …

Biogeochemical Controls On Biodegradation Of Mc252 Crude Oil In Coastal Wetland Systems, Matthew Paul Rodrigue

LSU Master's Theses

Weathering of MC252 oil from the Deepwater Horizon oil spill is taking place in coastal Louisiana marsh and mangrove wetland systems. Alkylated phenanthrenes and dibenzothiophenes, which are the most prominent PAHs found at MC252 contaminated sites, are weathering at rates up to 5% per year relative to alkylated chrysenes 3 years after initial oiling. Flux chambers at marsh locations trapped CO2 that exhibited δ13C signatures lower than indigenous organic matter signifying hydrocarbon mineralization to CO2. Weathering rates at each of these locations are heavily influenced by the biogeochemistry of the surrounding environment. High-resolution nutrient profiles were obtained at different locations …

Plants As Bio-Indicators Of Subsurface Conditions: Impact Of Groundwater Level On Btex Concentrations In Trees, Jordan Wilson, Rachel Bartz, Matt Limmer, Joel Gerard Burken

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Numerous Studies Have Demonstrated Trees' Ability to Extract and Translocate Moderately Hydrophobic Contaminants, and Sampling Trees for Compounds Such as BTEX Can Help Delineate Plumes in the Field. However, When BTEX is Detected in the Groundwater, Detection in Nearby Trees is Not as Reliable an Indicator of Subsurface Contamination as Other Compounds Such as Chlorinated Solvents. Aerobic Rhizospheric and Bulk Soil Degradation is a Potential Explanation for the Observed Variability of BTEX in Trees as Compared to Groundwater Concentrations. the Goal of This Study Was to Determine the Effect of Groundwater Level on BTEX Concentrations in Tree Tissue. the Central …

Evaluation Of Anaerobic Biodegradation Of High Concentrations Of Halogenated Methanes, Priya Rachel Jacob

All Theses

Removal of the chlorinated methanes, carbon tetrachloride (CT), chloroform (CF) and dichloromethane (DCM), and the halogenated methane, trichlorofluoromethane (commonly known as CFC-11 or Freon-11) from contaminated soil and groundwater remains challenging. Releases to the environment resulted from historically poor disposal practices, as well as spills and leaks from underground storage tanks. Among these three compounds, CF is considered the benchmark for evaluating the feasibility of bioremediation, due to its typically high level of toxicity towards most anaerobic prokaryotes. However, inhibitory effects of low concentrations of CFC-11 on anaerobes have been reported as well. Bioremediation is an attractive option for clean-up, …

Evaluation Of The Biodegradability And Toxicity Of Pca And Mpca, Juan Rueda

Electronic Theses and Dissertations

The main types of hypergolic propellants used at Kennedy Space Center (KSC) are hydrazine (HZ) and monomethylhydrazine (MMH). HZ and MMH are classified as hazardous materials and they are also known to be potentially carcinogenic to humans; therefore, handling these substances and their waste is strictly regulated. The wastes streams from HZ and MMH have been estimated to be the main hazardous wastes streams at KSC. Currently at KSC these wastes are first neutralized using citric acid and then they are transported on public roads for incineration as hazardous materials. A new method using alpha ketoglutaric acid (AKGA) was proposed …

An Efficient Biotreatment Process For Polyvinyl Alcohol Containing Textile Wastewater, Sandip S. Magdum, Gauri P. Minde, Upendra S. Adhyapak, Kalyanraman V.

Sandip S. Magdum

The aim of this work was to optimize the biodegradation of polyvinyl alcohol (PVA) containing actual textile wastewater for a sustainable treatment solution. The isolated microbial consortia of effective PVA degrader namely Candida Sp. and Pseudomonas Sp., which were responsible for symbiotic degradation of chemical oxidation demand (COD) and PVA from desizing wastewater. In the process optimization, the maximum aeration was essential to achieve a high degradation rate, where as stirring enhances further degradation and foam control. Batch experiments concluded with the need of 16 lpm/l and 150 rpm of air and stirring speed respectively for high rate of COD …

Plants As Bio-Indicators Of Subsurface Conditions: Impact Of Groundwater Level On Btex Concentrations In Trees, Jordan Wilson, Rachel Bartz, Matt Limmer, Joel Gerard Burken

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Numerous Studies Have Demonstrated Trees' Ability to Extract and Translocate Moderately Hydrophobic Contaminants, and Sampling Trees for Compounds Such as BTEX Can Help Delineate Plumes in the Field. However, When BTEX is Detected in the Groundwater, Detection in Nearby Trees is Not as Reliable an Indicator of Subsurface Contamination as Other Compounds Such as Chlorinated Solvents. Aerobic Rhizospheric and Bulk Soil Degradation is a Potential Explanation for the Observed Variability of BTEX in Trees as Compared to Groundwater Concentrations. the Goal of This Study Was to Determine the Effect of Groundwater Level on BTEX Concentrations in Tree Tissue. the Central …

Bioremediation Of Chlorinated Ethanes And Ethenes In Vertical Flow Engineered Wetland Systems, Christopher Akudo

LSU Doctoral Dissertations

Sustainable treatment of chlorinated ethanes and ethenes contaminated groundwater using vertical flow engineered wetland systems were investigated in microcosm and column studies. Experiments on environmental and biogeochemical factors that affect system performance were conducted, and a numerical model involving advection, sorption, and sequential biodegradation was developed to describe the fate and transport of the contaminants of concern in the treatment wetland bed. 1,1-dichloroethane (1,1-DCA) and cis-1,2-dichloroethene (cis-1,2-DCE) were used as the chemicals of interest. The presence of cis-1,2-DCE inhibited dechlorination of 1,1-DCA but cis-1,2-DCE dechlorination was not affected by the presence of 1,1-DCA. Simulation runs showed that the treatment bed …

Degradation And Inhibition Of Chlorinated Ethanes And Ethenes In A Two Bed, Sequential Bioremediation System, Alex Boudreau

LSU Master's Theses

A continuous-flow system composed of two anaerobic bioreactor (ABR) columns (based on engineered wetland systems) connected in series was tested to observe the ability of such a system to treat multiple volatile organic compounds (VOCs) possessing suspected inhibitory effects on the dechlorination of other compounds with cis-1,2-dichloroethylene (cDCE) and 1,1-dichloroethane (1,1-DCA) as the main test chemicals. Following the establishment of cDCE and 1,1-DCA dechlorinating ability at baseline conditions, system perturbations of breakthrough and starvation were conducted to measure the response and recovery of the system dechlorination. In all column experiments, it was observed cDCE dechlorination inhibited the dechlorination of 1,1-DCA …

Applied And Mechanistic Studies Of Microbial 17beta-Estradiol Degradation, Zhongtian Li

Z Li

The presence of natural estrogens, a class of endocrine disrupting compounds, in water has caused increasing concerns over their adverse impacts on the health of aquatic eco-systems and human beings. In this study, adsorption characteristics of two natural estrogens, 17β-estradiol (E2) and estrone (E1), on granular activated carbon (GAC) were investigated in isotherm tests and in a GAC column. The GAC column was then converted to a biologically active carbon (BAC) column and the removal efficiency of E2 and its primary biodegradation intermediate E1 were monitored. During BAC operation, the impacts of various reactor operation parameters, such as the carbon …

Effects Of Moisture Augmentation Of Municipal Solid Waste Through Addition Of Food Waste Or Wastewater Treatment Biosolids On Bio-Gas Formation For Power Generation, Jared Michael Gore

UNLV Theses, Dissertations, Professional Papers, and Capstones

An investigation into the effect of moisture augmentation by manipulation of food waste proportion or wastewater treatment plant biosolids proportion was undertaken to determine the effects on production of methane and other biogases from municipal solid waste (MSW). Laboratory microcosm experiments were performed to determine the effect of various proportions of influent waste streams on the production of biogas. Results indicated that moisture augmentation through the addition of food waste to MSW increases the overall bio-gas and hydrogen gas formed during fermentation. Moisture augmentation through addition of wastewater treatment bio-solids lead to inconclusive results. Addition of food waste to MSW …

Proteomic Analysis Of 17Β-Estradiol Degradation By Stenotrophomonas Maltophilia, Zhongtian Li

Z Li

Microbial degradation plays a critical role in determining the environmental fate of steroid hormones, such as 17β-estradiol (E2). The molecular mechanisms governing the microbial transformation of E2 and its primary degradation intermediate, estrone (E1), are largely unknown. The objective of this study was to identify metabolism pathways that might be involved in microbial estrogen degradation. To achieve the objective, Stenotrophomonas maltophilia strain ZL1 was used as a model estrogen degrading bacterium and its protein expression level during E2/E1 degradation was studied using quantitative proteomics. During an E2 degradation experiment, strain ZL1 first converted E2 to E1 stoichiometrically. At 16 h …

Biodegradation Of Organophosphate Chemical Warfare Agents By Activated Sludge, Steven J. Schuldt

Theses and Dissertations

Organophosphates (OPs) have been widely used as Chemical Warfare Agents (CWAs) as well as pesticides since World War II and still remain a threat to national security. While efforts have been taken at military installations and civilian communities to secure these chemicals and prevent their misuse, a determined adversary could still obtain and deploy them to injure, kill, or instill terror. The lethal properties of this group of compounds are primarily owed to their irreversible inhibition of the enzyme acetyl cholinesterase (AChE) and thus may alter the human nervous system or affect the hormonal balance of children in particular. In …

Applied And Mechanistic Studies Of Microbial 17beta-Estradiol Degradation, Zhongtian Li

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

The presence of natural estrogens, a class of endocrine disrupting compounds, in water has caused increasing concerns over their adverse impacts on the health of aquatic eco-systems and human beings. In this study, adsorption characteristics of two natural estrogens, 17β-estradiol (E2) and estrone (E1), on granular activated carbon (GAC) were investigated in isotherm tests and in a GAC column. The GAC column was then converted to a biologically active carbon (BAC) column and the removal efficiency of E2 and its primary biodegradation intermediate E1 were monitored. During BAC operation, the impacts of various reactor operation parameters, such as the carbon …

Biodegradation Kinetics For 1,2-Dichloroethane And Ethylene Dibromide In Anaerobic Enrichment Cultures Grown On Each Compound, Rong Yu

All Theses

1,2-Dichloroethane (DCA) and ethylene dibromide (EDB) are among the top 15 chlorinated aliphatic compounds on the Agency for Toxic Substances and Disease Registry's Priority List of Hazardous Substances. Co-contamination of groundwater with EDB and 1,2-DCA resulted mainly from environmental releases of leaded gasoline; these compounds were added as lead scavengers. A microcosm study by Henderson et al. with soil and groundwater from a site contaminated with leaded gasoline demonstrated anaerobic biodegradation of EDB at a higher rate, and to a greater extent, than 1,2-DCA. Both compounds were transformed mainly by dihaloelimination to ethene. The objectives of this study were to …

Biological Treatment To Remove Color And Toxicity Of Effluent From The Manufacturing Of Navipol Yellow Gr, Larry French

All Theses

Navipol Yellow GR is an organic solvent azo dye manufactured by Sunbelt Corp. in Rock Hill, SC. The effluent generated from the manufacturing of this dye is highly colored, toxic, and contains trace solvents consisting of mixed xylene isomers and ethyl benzene. Traditional wastewater treatment systems are unable to remove the color and toxicity from the effluent. This study investigated using a sequential anaerobic / aerobic treatment system to decrease color and toxicity of the dye effluent. The anaerobic phase used bacteria cultures of Pseudomonas aeruginosa, Bacillus subtilis, Enterococcus faecalis, or Enterobacter aerogenes to decrease the color of the dye. …

Biodegradation Of The High Explosive Hexanitrohexaazaiso-Wurtzitane (Cl-20), Pelin Karakaya, Christos Christodoulatos, Agamemnon Koutsospyros, Wendy Balas, Steve Nicolich Nicolich, Mohammed Sidhoum

Civil Engineering Faculty Publications

The aerobic biodegradability of the high explosive CL-20 by activated sludge and the white rot fungus Phanerochaete chrysosporium has been investigated. Although activated sludge is not effective in degrading CL-20 directly, it can mineralize the alkaline hydrolysis products. Phanerochaete chrysosporium degrades CL-20 in the presence of supplementary carbon and nitrogen sources. Biodegradation studies were conducted using various nutrient media under diverse conditions. Variables included the CL-20 concentration; levels of carbon (as glycerol) and ammonium sulfate and yeast extract as sources of nitrogen. Cultures that received CL-20 at the time of inoculation transformed CL-20 completely under all nutrient conditions studied. When …