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Dynamic Growth Rates Of Microbial Populations In Activated Sludge Systems, Peter George Stroot, Pascal E. Saikaly, Daniel B. Oerther
Dynamic Growth Rates Of Microbial Populations In Activated Sludge Systems, Peter George Stroot, Pascal E. Saikaly, Daniel B. Oerther
Civil, Architectural and Environmental Engineering Faculty Research & Creative Works
Results of mathematical modeling and whole cell 16S ribosomal RNA-targeted fluorescence in situ hybridizations challenge the widely held perception that microbial populations in "steady-state" activated sludge systems share a common net growth rate that is proportional to the inverse of the mean cell residence time. Our results are significant because they encourage bioprocess engineers to appreciate the differences in growth physiology among individual microbial populations in complex mixed microbial communities such as suspended growth activated sludge bioreactor systems.
Use Of 16s Rrna Gene Terminal Restriction Fragment Analysis To Assess The Impact Of Solids Retention Time On The Bacterial Diversity Of Activated Sludge, Pascal E. Saikaly, Peter George Stroot, Daniel B. Oerther
Use Of 16s Rrna Gene Terminal Restriction Fragment Analysis To Assess The Impact Of Solids Retention Time On The Bacterial Diversity Of Activated Sludge, Pascal E. Saikaly, Peter George Stroot, Daniel B. Oerther
Civil, Architectural and Environmental Engineering Faculty Research & Creative Works
Terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes was used to investigate the reproducibility and stability in the bacterial community structure of laboratory-scale sequencing batch bioreactors (SBR) and to assess the impact of solids retention time (SRT) on bacterial diversity. Two experiments were performed. In each experiment two sets of replicate SBRs were operated for a periods of three times the SRT. One set was operated at an SRT of 2 days and another set was operated at an SRT of 8 days. Samples for T-RFLP analysis were collected from the two sets of replicate reactors. HhaI, …