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Civil and Environmental Engineering

University of Nebraska - Lincoln

2016

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Invasive Species Impacts On Infrastructure, Invasive Species Advisory Committee Dec 2016

Invasive Species Impacts On Infrastructure, Invasive Species Advisory Committee

National Invasive Species Council

Executive Summary

Invasive species represent one of the most significant threats to ecosystems, human and animal health, infrastructure, the economy, and cultural resources. Because potentially invasive, non-native species typically enter the United States through ports of entry in urban environments, some of the first observable impacts may be to infrastructure, yet little is known about the economic costs associated with these impacts to the “built” environment. In addition, federal agencies currently lack the authority necessary to effectively prevent, eradicate, and control invasive species that impact the human-built environment. This lack of authority prevents rapid response to some of the most …


Evaluation Of Dowel Bar Inserter Practices In Pcc Pavements With Magnetic Tomography Technology, Farshad Fallah, Yong-Rak Kim Dec 2016

Evaluation Of Dowel Bar Inserter Practices In Pcc Pavements With Magnetic Tomography Technology, Farshad Fallah, Yong-Rak Kim

Nebraska Department of Transportation: Research Reports

Dowel Bar Inserters (DBI) are automated mechanical equipment that position dowel bars in Portland Cement Concrete (PCC) after concrete is placed. Compared to the alternative approach, which is using dowel baskets, DBIs offer advantages in cost and speed of construction. However, as dowel bars are not anchored to the subgrade similar to dowel baskets, there is a concern about the quality of dowel placement using this equipment. Improper placement of dowel bars can lead to reduced load transfer between slabs, which results in pavement distresses such as faulting and spalling at joints. To determine the accuracy of dowel placement by …


M049 Effects Of Compaction And Soil Moisture On American Burying Beetles, W. Wyatt Hoback Dec 2016

M049 Effects Of Compaction And Soil Moisture On American Burying Beetles, W. Wyatt Hoback

Nebraska Department of Transportation: Research Reports

Silphid beetles in the genus Nicrophorus bury themselves during periods of inactivity, however, the influence of soil characteristics on burial behavior remains unclear. We examined soil preferences of the federally endangered Nicrophorus americanus, as well as of N. carolinus, N. marginatus, N. obscurus, and N. orbicollis, in a series of experiments. Differences in burial depth by sex were found for N. carolinus, N. marginatus, and N. obscurus, which are diurnal species. Both sexes of N. americanus bury to a mean depth of 18 cm. Soil preferences tested included sand versus sandy loam versus sandy loess, degrees of soil moisture, and …


Continuous Long-Term Health Monitoring Using Ultrasonic Wave Propagation, Ece Erdogmus Dec 2016

Continuous Long-Term Health Monitoring Using Ultrasonic Wave Propagation, Ece Erdogmus

Nebraska Department of Transportation: Research Reports

No abstract provided.


Effectiveness Study Of Unl’S Crack Routing Device (Crd) –Phase Ii, Changbum Ahn, Yong Cho, Hamed N. Rafsanjani, Heath Wiley Dec 2016

Effectiveness Study Of Unl’S Crack Routing Device (Crd) –Phase Ii, Changbum Ahn, Yong Cho, Hamed N. Rafsanjani, Heath Wiley

Nebraska Department of Transportation: Research Reports

This project evaluated the crack routing device (CRD) for effectiveness in improving current crack-cutting practices and for possible adoption as a standard in the Nebraska Department of Roads (NDOR). To enact these evaluations of CRD, the research team collaborated with NDOR to conduct surveys and field tests. Given the positive results obtained from this project, improved road-maintenance practices are anticipated when CRD is used, and this research therefore recommends the adoption of CRD in crack-routing work.


Condition Factor Calibration For Load And Resistance Factor Rating Of Steel Girder Bridges, Pranav M. Shakya Dec 2016

Condition Factor Calibration For Load And Resistance Factor Rating Of Steel Girder Bridges, Pranav M. Shakya

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

The Load and Resistance Factor Rating (LRFR) is a reliability-based rating procedure complementary to the Load and Resistance Factor Design (LRFD). The intent of LRFR is to provide consistent reliability for all bridges regardless of in-situ condition. The primary difference between design and rating is the uncertain severity and location of deterioration, including the potential future loss of strength for an element already evidencing deterioration. Ostensibly, these uncertainties are accounted for by applying an additional strength reduction factor: the condition factor, ϕc. Currently, condition factors are nominally correlated to the condition of the member, which can be Good, …


Spatial Distribution Of Antibiotic Resistance In Soils Receiving Beef Feedlot Runoff, Scott Speicher Dec 2016

Spatial Distribution Of Antibiotic Resistance In Soils Receiving Beef Feedlot Runoff, Scott Speicher

Department of Environmental Engineering: Theses and Student Research

A study was conducted to provide new insight on the potential contribution to antibiotic resistance from the land application of beef feedlot runoff to soil. This study reports the distribution and quantity of antibiotic resistant bacteria (ARBs), fecal indicator bacteria (FIB), and antibiotic resistance genes (ARGs) in soil from (i) a field receiving long-term application of beef feedlot runoff holding pond effluent and (ii) a cool-season pasture with no history of supplemental manure application.

Soil samples were collected June 2015 at the U.S. Meat Animal Research Center near Clay Center, Nebraska. A response surface sampling design (RSSD) model based on …


Modeling Streambank Erosion On Composite Streambanks On A Watershed Scale, A. R. Mittelstet, D. E. Storm, G. A. Fox, P. M. Allen Dec 2016

Modeling Streambank Erosion On Composite Streambanks On A Watershed Scale, A. R. Mittelstet, D. E. Storm, G. A. Fox, P. M. Allen

Department of Biological Systems Engineering: Papers and Publications

Streambanks can be a significant source of sediment and phosphorus to aquatic ecosystems. Although the streambank-erosion routine in the Soil and Water Assessment Tool (SWAT) has improved in recent versions, the recently developed routine in SWAT 2012 has undergone limited testing, and the lack of site or watershed specific streambank data increases the uncertainty in the streambank-erosion predictions. There were two primary objectives of this research: (1) modify and test the 2012 SWAT streambank-erosion routine on composite streambanks, and (2) compare SWAT default and field-measured channel parameters and assess their influence on predicted streambank erosion. Three modifications were made to …


Species Distribution Model For Swift Fox In Nebraska, Lucia Corral Hurtado Nov 2016

Species Distribution Model For Swift Fox In Nebraska, Lucia Corral Hurtado

Nebraska Department of Transportation: Research Reports

No abstract provided.


Quantifying An Aquifer Nitrate Budget And Future Nitrate Discharge Using Field Data From Streambeds And Well Nests, Troy E. Gilmore, David P. Genereux, Kathleen M. Farrell, Helena Mitasova Nov 2016

Quantifying An Aquifer Nitrate Budget And Future Nitrate Discharge Using Field Data From Streambeds And Well Nests, Troy E. Gilmore, David P. Genereux, Kathleen M. Farrell, Helena Mitasova

Department of Biological Systems Engineering: Papers and Publications

Novel groundwater sampling (age, flux, and nitrate) carried out beneath a streambed and in wells was used to estimate (1) the current rate of change of nitrate storage, dSNO3 /dt, in a contaminated unconfined aquifer, and (2) future [NO3]FWM (the flow-weighted mean nitrate concentration in groundwater discharge) and fNO3 (the nitrate flux from aquifer to stream). Estimates of dSNO3 /dt suggested that at the time of sampling (2013) the nitrate storage in the aquifer was decreasing at an annual rate (mean = –9 mmol/m2yr) equal …


Benchmark Levels For The Consumptive Water Footprint Of Crop Production For Different Environmental Conditions: A Case Study For Winter Wheat In China, La Zhuo, Mesfin Mekonnen, Arjen Y. Hoekstra Nov 2016

Benchmark Levels For The Consumptive Water Footprint Of Crop Production For Different Environmental Conditions: A Case Study For Winter Wheat In China, La Zhuo, Mesfin Mekonnen, Arjen Y. Hoekstra

Daugherty Water for Food Global Institute: Faculty Publications

Meeting growing food demands while simultaneously shrinking the water footprint (WF) of agricultural production is one of the greatest societal challenges. Benchmarks for the WF of crop production can serve as a reference and be helpful in setting WF reduction targets. The consumptive WF of crops, the consumption of rainwater stored in the soil (green WF), and the consumption of irrigation water (blue WF) over the crop growing period varies spatially and temporally depending on environmental factors like climate and soil. The study explores which environmental factors should be distinguished when determining benchmark levels for the consumptive WF of crops. …


Anisotropic Contrast Optical Microscope, D. Peev, T. Hofmann, N. Kananizadeh, S. Beeram, E. Rodriguez, S. Wimer, K.B. Rodenhausen, C.M. Herzinger, T. Kasputis, E. Pfaunmiller, A. Nguyen, Rafal Korlacki, A. Pannier, Y. Li, E. Schubert, D. Hage, M. Schubert Nov 2016

Anisotropic Contrast Optical Microscope, D. Peev, T. Hofmann, N. Kananizadeh, S. Beeram, E. Rodriguez, S. Wimer, K.B. Rodenhausen, C.M. Herzinger, T. Kasputis, E. Pfaunmiller, A. Nguyen, Rafal Korlacki, A. Pannier, Y. Li, E. Schubert, D. Hage, M. Schubert

Department of Civil and Environmental Engineering: Faculty Publications

An optical microscope is described that reveals contrast in the Mueller matrix images of a thin, transparent, or semi-transparent specimen located within an anisotropic object plane (anisotropic filter). The specimen changes the anisotropy of the filter and thereby produces contrast within the Mueller matrix images. Here we use an anisotropic filter composed of a semi-transparent, nanostructured thin film with sub-wavelength thickness placed within the object plane. The sample is illuminated as in common optical microscopy but the light is modulated in its polarization using combinations of linear polarizers and phase plate (compensator) to control and analyze the state of polarization. …


Effect Of Rainfall Timing And Tillage On The Transport Of Steroidhormones In Runoff From Manure Amended Row Crop Fields, Sagor Biswas, William L. Kranz, Charles A. Shapiro, Daniel D. Snow, Shannon L. Bartelt-Hunt, Mitiku Mamo, David D. Tarkalson, Tian C. Zhang, David P. Shelton, Simon J. Van Donk, Terry L. Mader Nov 2016

Effect Of Rainfall Timing And Tillage On The Transport Of Steroidhormones In Runoff From Manure Amended Row Crop Fields, Sagor Biswas, William L. Kranz, Charles A. Shapiro, Daniel D. Snow, Shannon L. Bartelt-Hunt, Mitiku Mamo, David D. Tarkalson, Tian C. Zhang, David P. Shelton, Simon J. Van Donk, Terry L. Mader

Department of Biological Systems Engineering: Papers and Publications

Runoff generated from livestock manure amended row crop fields is one of the major pathways of hormone transport to the aquatic environment. The study determined the effects of manure handling, tillage methods, and rainfall timing on the occurrence and transport of steroid hormones in runoff from the row crop field. Stockpiled and composted manure from hormone treated and untreated animals were applied to test plots and subjected to two rainfall simulation events 30 days apart. During the two rainfall simulation events, detection of any steroid hormone or metabolites was identified in 8–86% of runoff samples from any tillage and manure …


How Acoustics In California High Performance Schools Relate To Student Achievement, Devin K. Wong Nov 2016

How Acoustics In California High Performance Schools Relate To Student Achievement, Devin K. Wong

Durham School of Architectural Engineering and Construction: Dissertations, Thesis, and Student Research

This research project seeks to determine if students attending K-12 schools meeting a minimum standard of high performance classroom conditions in the state of California do evidence higher scholastic achievement, based on publically available results on state-wide standardized tests. In three phases, a subset of 200+ schools applying for grants dedicated to building high performance schools over the past decade was correlated with an existing database of achievement scores for all public schools in California. The criteria utilized to specify high performance classrooms was provided by the California High Performance Initiative (HPI) Grant program. Academic achievement was evaluated on a …


Development Of A Transition Between An Energy-Absorbing Concrete Barrier And A Rigid Concrete Butress, Tyler L. Schmidt, Ronald K. Faller, Jennifer D. Schmidt, John D. Reid, Robert W. Bielenberg, Scott Rosenbaugh Sep 2016

Development Of A Transition Between An Energy-Absorbing Concrete Barrier And A Rigid Concrete Butress, Tyler L. Schmidt, Ronald K. Faller, Jennifer D. Schmidt, John D. Reid, Robert W. Bielenberg, Scott Rosenbaugh

Nebraska Department of Transportation: Research Reports

From 2010 to 2015, MwRSF researchers developed the RESTORE barrier, which is a restorable MASH TL-4 median barrier with a steel and concrete rail supported by elastomer posts and steel skids. The research effort reported herein describes the initial development of a transition from the RESTORE barrier to a rigid TL-4 concrete buttress.

The previously-developed RESTORE barrier LS-DYNA model was validated against three full-scale vehicle crash tests. Several design concepts were generated through a series of brainstorming efforts. The primary transition concept consisted of a pin and loop connection between the RESTORE barrier and rigid concrete buttress, which was designed …


Research On High-Rap Asphalt Mixtures With Rejuvenators And Wma Additives, Hamzeh Haghshenas, Hesanaddin Nabizadeh, Yong-Rak Kim, Kommidi Santosh Sep 2016

Research On High-Rap Asphalt Mixtures With Rejuvenators And Wma Additives, Hamzeh Haghshenas, Hesanaddin Nabizadeh, Yong-Rak Kim, Kommidi Santosh

Nebraska Department of Transportation: Research Reports

No abstract provided.


Performance Of Advance Warning Systems In A Coordinated System, Li Zhao, Laurence R. Rilett, Ernest Tufuor, Waleed Khan, Christopher Lefrois Sep 2016

Performance Of Advance Warning Systems In A Coordinated System, Li Zhao, Laurence R. Rilett, Ernest Tufuor, Waleed Khan, Christopher Lefrois

Nebraska Department of Transportation: Research Reports

The Advance Warning System (AWS), developed by the Nebraska Department of Roads (NDOR) has proven to be effective at improving traffic safety at isolated signalized intersections. However, the effectiveness of the system has not been analyzed at signalized intersections operating in a coordinated mode. This project analyzed AWS on arterials where the signals operate in a coordinated mode. The test bed consisted of nine sites, which are located at five successive coordinated signalized intersections on Highway 281 in Grand Island, Nebraska. A non-intrusive data collection system was used to collect a continuous traffic stream of data up to 1200 ft …


Wetlands And Coastal Systems: Protecting And Restoring Valuable Ecosystems, C. T. Agouridis, K. R. Douglas-Mankin, A. C. Linhoss, A. R. Mittelstet Sep 2016

Wetlands And Coastal Systems: Protecting And Restoring Valuable Ecosystems, C. T. Agouridis, K. R. Douglas-Mankin, A. C. Linhoss, A. R. Mittelstet

Department of Biological Systems Engineering: Papers and Publications

Wetlands and coastal systems are unique, highly productive, and often threatened landscapes that provide a host of services to both humans and the environment. This article introduces a five-article Wetlands and Coastal Systems Special Collection that evolved from a featured session at the 2015 ASABE Annual International Meeting in New Orleans, Louisiana. The Collection provides perspectives on tools and techniques for enhancing the protection and restoration of wetlands and coastal systems with emphasis on vegetation, hydrology, water quality, and planning. Topics span the Florida Everglades (two articles) and Virginia floodplain (one article) wetland systems and include remote sensing (one article) …


Improved Methodologies In Modeling And Predicting Failure In Aashto M-180 Guardrail Steel Using Finite Element Analysis – Phase I, Brandt M. Humphrey, Robert W. Bielenberg, Ronald K. Faller, John D. Reid, Mehrdad Negahban Aug 2016

Improved Methodologies In Modeling And Predicting Failure In Aashto M-180 Guardrail Steel Using Finite Element Analysis – Phase I, Brandt M. Humphrey, Robert W. Bielenberg, Ronald K. Faller, John D. Reid, Mehrdad Negahban

Nebraska Department of Transportation: Research Reports

No abstract provided.


Midwest Guardrail System (Mgs) Placed Adjacent To A 1v:2h Fill Slope With 6-Ft Posts, Austin J. Haase, Justine E. Kohtz, Karla A. Lechtenberg, John D. Reid, Ronald K. Faller Aug 2016

Midwest Guardrail System (Mgs) Placed Adjacent To A 1v:2h Fill Slope With 6-Ft Posts, Austin J. Haase, Justine E. Kohtz, Karla A. Lechtenberg, John D. Reid, Ronald K. Faller

Nebraska Department of Transportation: Research Reports

No abstract provided.


Identifying And Addressing Soil Property Issues Affecting Roadside Vegetation Establishment, Xu Li, Martha Mamo, Walter H. Schacht, Tala Awada, Humberto Blanco-Canqui Aug 2016

Identifying And Addressing Soil Property Issues Affecting Roadside Vegetation Establishment, Xu Li, Martha Mamo, Walter H. Schacht, Tala Awada, Humberto Blanco-Canqui

Nebraska Department of Transportation: Research Reports

Attaining adequate vegetation cover along highways is important for NDOR to comply with EPA’s stormwater regulations. However, low plant cover is a common problem on shoulders (first 16 feet off the pavement) of many highways in Nebraska. The ultimate goal of this study is to identify cost-effective engineering solutions that assure adequate seed beds (i.e., soil conditions) for establishment of selected seeding mixtures. The objectives of this study are to (1) characterize the soil properties along roadsides where vegetation stands have not developed well, and (2) verify the effects of select soil property parameters on plant germination and establishment.

Sampling …


Design Procedures Of Retrofitted Bridge Rail, Richard L. Wood, Yijun Liao, Mohammad E. Mohammadi, Jordan Wipf Aug 2016

Design Procedures Of Retrofitted Bridge Rail, Richard L. Wood, Yijun Liao, Mohammad E. Mohammadi, Jordan Wipf

Nebraska Department of Transportation: Research Reports

The use of shallow embedment anchors in concrete is essential for retrofit applications; however, the combination of steel anchors, chemical adhesive, and concrete acting as a single tensile mechanism creates uncertainties in anchor strength and failure modes. Differences in concrete compressive strengths and in specifications of adhesives between manufacturers increases uncertainty further. In addition to these issues, prior research conducted by others examines the behavior of such anchors with respect to cracked versus uncracked concrete, partial bonding of the embedded length, and strength reductions due to anchor proximity to edges. While it is understood, anchors require a proper installation detail, …


Laboratory And Full Boom-Based Investigation Of Nozzle Setup And Restriction Effects On Flow, Pressure And Spray Pattern Distribution, Shane H. Forney Aug 2016

Laboratory And Full Boom-Based Investigation Of Nozzle Setup And Restriction Effects On Flow, Pressure And Spray Pattern Distribution, Shane H. Forney

Department of Biological Systems Engineering: Dissertations and Theses

Pesticide application is an integral part of crop production and ground-based agricultural boom sprayers are used extensively to apply pesticides to the crop canopy or soil surface across millions of acres in the United States. Efficient application is necessary to minimize costs and limit adverse environmental impacts. Errors in flow rate and system pressure measurements may cause as-applied maps to incorrectly indicate application rates and could negatively affect downstream data processing or analysis. The goals of this study were to provide quantified measurements on the effects of nozzle setup errors on spray pattern uniformity and evaluate how laboratory patternator data …


Unmanned Aerial Vehicles For High-Throughput Phenotyping And Agronomic Research, Yeyin Shi, J. Alex Thomasson, Seth C. Murray, N. Ace Pugh, William L. Rooney, Sanaz Shafian, Nithya Rajan, Gregory Rouze, Cristine L. S. Morgan, Haly L. Neely, Aman Rana, Muthu V. Bagavathiannan, James Henrickson, Ezekiel Bowden, John Valasek, Jeff Olsenholler, Michael P. Bishop, Ryan Sheridan, Eric B. Putman, Sorin Popescu, Travis Burks, Dale Cope, Amir Ibrahim, Billy F. Mccutchen, David D. Baltensperger, Robert V. Avant, Jr., Misty Vidrine, Chenghai Yang Jul 2016

Unmanned Aerial Vehicles For High-Throughput Phenotyping And Agronomic Research, Yeyin Shi, J. Alex Thomasson, Seth C. Murray, N. Ace Pugh, William L. Rooney, Sanaz Shafian, Nithya Rajan, Gregory Rouze, Cristine L. S. Morgan, Haly L. Neely, Aman Rana, Muthu V. Bagavathiannan, James Henrickson, Ezekiel Bowden, John Valasek, Jeff Olsenholler, Michael P. Bishop, Ryan Sheridan, Eric B. Putman, Sorin Popescu, Travis Burks, Dale Cope, Amir Ibrahim, Billy F. Mccutchen, David D. Baltensperger, Robert V. Avant, Jr., Misty Vidrine, Chenghai Yang

Department of Biological Systems Engineering: Papers and Publications

Advances in automation and data science have led agriculturists to seek real-time, high quality, high-volume crop data to accelerate crop improvement through breeding and to optimize agronomic practices. Breeders have recently gained massive data-collection capability in genome sequencing of plants. Faster phenotypic trait data collection and analysis relative to genetic data leads to faster and better selections in crop improvement. Furthermore, faster and higher-resolution crop data collection leads to greater capability for scientists and growers to improve precision-agriculture practices on increasingly larger farms; e.g., site-specific application of water and nutrients. Unmanned aerial vehicles (UAVs) have recently gained traction as agricultural …


Development And Assessment Of A Groundwater Sustainability Index In Climatically Diverse Groundwater Irrigated Regions In Nebraska, Maria A. Mulet Jalil Jul 2016

Development And Assessment Of A Groundwater Sustainability Index In Climatically Diverse Groundwater Irrigated Regions In Nebraska, Maria A. Mulet Jalil

Department of Biological Systems Engineering: Dissertations and Theses

The aim of this research was to evaluate the impact of regional change in ET on groundwater level changes and the assessment and development of a groundwater sustainability index for climatically diverse regions across Nebraska during 2000-2014. Irrigation in the selected regions is predominantly supplied by groundwater. The hypothesis is that groundwater use can become sustainable if the regional evapotranspiration (ET) is managed so that it equals the ET of vegetation that is native to the region. Site locations were Box Butte, Chase, Dundy, Holt LNNRD and York Counties and 3 ecosystems were evaluated: native vegetation, dryland and irrigated cropping …


Experimental Modal Analysis And Seismic Mitigation Of Statue-Pedestal Systems, Christine E. Wittich, Tara C. Hutchinson Jul 2016

Experimental Modal Analysis And Seismic Mitigation Of Statue-Pedestal Systems, Christine E. Wittich, Tara C. Hutchinson

Department of Civil and Environmental Engineering: Faculty Publications

The seismic protection of cultural heritage, particularly statues, is a critical issue due to its high cultural significance, difficulty to repair or replace artifacts, and observed poor behavior during past earthquakes. Recent research has explored analysis techniques and methodologies for predicting the seismic response of statues; however, these studies typically assume the statue to be either freestanding or rigidly attached. The seismic response of statues with these different boundary conditions varies widely and therefore accurate characterization is critical. While modern mounting techniques aim to rigidly attach a statue to the floor or to a pedestal, the degree of rigidity of …


Use Of Recycled Crushed Concrete (Rcc) Fines For Potential Soil Stabilization, Lindeman Mark, Varilek Brandon, Lieska Halsey, Rick Gloe, Maria Olomi Jul 2016

Use Of Recycled Crushed Concrete (Rcc) Fines For Potential Soil Stabilization, Lindeman Mark, Varilek Brandon, Lieska Halsey, Rick Gloe, Maria Olomi

Nebraska Department of Transportation: Research Reports

This study evaluated the use of Recycled Crushed Concrete (RCC) Fines for potential soil stabilization. Soil stabilization is the enhancement of subgrade stability to improve the constructability of successive pavement layers. Use of RCC fines may not only provide less costly alternatives for subgrade stabilization, but their use may also alleviate landfill disposal challenges.


Cfd Study Of Decay Function Of Wall Shear Stress With Scour Around Complex-Shape Bridge Pier, Chen Li Jul 2016

Cfd Study Of Decay Function Of Wall Shear Stress With Scour Around Complex-Shape Bridge Pier, Chen Li

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Pier scour problem is close related to the safety of bridges. The Federal Highway Administration of the United State also has a great interest in studying the relation between hydraulic loadings and scour depth. The current technological problem of directly measuring hydraulic loadings on a dynamic bed using physical experiments and the weak capacity to simulate the real scour processes with CFD methods inspired the development of a hybrid approach by combining them to study pier scour.

This research specifically focuses on the CFD part of the hybrid method. A series of three-dimensional (3-D) CFD models were developed with unsteady …


The Experiment And Analysis Of Active Mechanisms For Enhancing Heat And Mass Transfer In Sorption Fluids, Ziqi Shen Jul 2016

The Experiment And Analysis Of Active Mechanisms For Enhancing Heat And Mass Transfer In Sorption Fluids, Ziqi Shen

Durham School of Architectural Engineering and Construction: Dissertations, Thesis, and Student Research

This project was funded by American Society of Heating, Refrigerating, and Air-Conditioning Engineers (ASHRAE RP-1462). It is a three years’ research, including the literature review, labs construction, experiments and data analysis.

In this thesis, first of all, we conducted literature review of mechanism motion influence on heat and mass transfer and additive effect in absorption chiller. This part helps us understand the basic idea of how mechanism motion affects the heat and mass transfer of sorption fluids and gives us reference on how to select the experiment instrument and the experiment operation range.

In the second part, the instrument selection …


High-Throughput Screening Of Clinically Approved Drugs That Prime Polyethylenimine Transfection Reveals Modulation Of Mitochondria Dysfunction Response Improves Gene Transfer Efficiencies, Albert Nguyen, Jared P. Beyersdorf, Jean-Jack Riethoven, Angela K. Pannier Jul 2016

High-Throughput Screening Of Clinically Approved Drugs That Prime Polyethylenimine Transfection Reveals Modulation Of Mitochondria Dysfunction Response Improves Gene Transfer Efficiencies, Albert Nguyen, Jared P. Beyersdorf, Jean-Jack Riethoven, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

Nonviral gene delivery methods are advantageous over viral vectors in terms of safety, cost, and flexibility in design and application, but suffer from lower gene transfer efficiency. In addition to modifications to nucleic acid design and nonviral carriers, new tools are sought to enhance transfection. Priming is the pharmacological modulation of transfection efficiency and transgene expression, and has demonstrated transfection increase in several compounds, for example, chloroquine and glucocorticoids. To develop a library of transfection priming compounds, a highthroughput screen was performed of the NIH Clinical Collection (NCC) to identify clinical compounds that prime polyethylenimine (PEI) transfection. HEK293T cells were …