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Civil and Environmental Engineering

Louisiana State University

Theses/Dissertations

2006

Continuous culture

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Design And Evaluation Of Automated, Continuous Culture Techniques For Brachionus Rotundiformis, Zhengzhong Zhang Jan 2006

Design And Evaluation Of Automated, Continuous Culture Techniques For Brachionus Rotundiformis, Zhengzhong Zhang

LSU Doctoral Dissertations

A 40-L recirculating rotifer turbidostat (RRT) pilot system consisting of a rotifer reactor, biofilter, foam fractionator, feed container, harvester, and two air-lift pumps was designed to produce B. rotundiformis, a small (S-type) rotifer, that is increasingly demanded for production of small-gape marine finfish larvae. The system’s automatic monitoring (turbidity, pH, conductivity and temperature) and controlling (turbidity, pH, feeding and harvesting) were performed by a Rugid™ computer. A 4 (temperature) x 3 (salinity) study indicated that a 30oC/15 ppt treatment is the most favorable to the growth of B. rotundiformis. Under these conditions, the effect of harvest rate on rotifer system …