Open Access. Powered by Scholars. Published by Universities.®
Civil and Environmental Engineering
Civil and Environmental Engineering Faculty Research and Publications
Articles 1 - 1 of 1
Full-Text Articles in Entire DC Network
Improved Virus Isoelectric Point Estimation By Exclusion Of Known And Predicted Genome-Binding Regions, Joe Heffron, Brooke Mayer
Improved Virus Isoelectric Point Estimation By Exclusion Of Known And Predicted Genome-Binding Regions, Joe Heffron, Brooke Mayer
Civil and Environmental Engineering Faculty Research and Publications
Knowledge of the isoelectric points (pIs) of viruses is beneficial for predicting virus behavior in environmental transport and physical/chemical treatment applications. However, the empirically measured pIs of many viruses have thus far defied simple explanation, let alone prediction, based on the ionizable amino acid composition of the virus capsid. Here, we suggest an approach for predicting the pI of nonenveloped viruses by excluding capsid regions that stabilize the virus polynucleotide via electrostatic interactions. This method was applied first to viruses with known polynucleotide-binding regions (PBRs) and/or three-dimensional (3D) structures. Then, PBRs were predicted in a group of 32 unique viral …