Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 7 of 7
Full-Text Articles in Entire DC Network
Quantitative Rrna-Targeted Solution-Based Hybridization Assay Using Peptide Nucleic Acid Molecular Beacons, Xu Li, Eberhard Morgenroth, Lutgarde Raskin
Quantitative Rrna-Targeted Solution-Based Hybridization Assay Using Peptide Nucleic Acid Molecular Beacons, Xu Li, Eberhard Morgenroth, Lutgarde Raskin
Department of Civil and Environmental Engineering: Faculty Publications
The potential of a solution-based hybridization assay using peptide nucleic acid (PNA) molecular beacon (MB) probes to quantify 16S rRNA of specific populations in RNA extracts of environmental samples was evaluated by designing PNA MB probes for the genera Dechloromonas and Dechlorosoma. In a kinetic study with 16S rRNA from pure cultures, the hybridization of PNA MB to target 16S rRNA exhibited a higher final hybridization signal and a lower apparent rate constant than the hybridizations to nontarget 16S rRNAs. A concentration of 10 mM NaCl in the hybridization buffer was found to be optimal for maximizing the difference between …
Application Of The Modified Log-Wake Law In Open-Channels, Junke Guo, Pierre Y. Julien
Application Of The Modified Log-Wake Law In Open-Channels, Junke Guo, Pierre Y. Julien
Department of Civil and Environmental Engineering: Faculty Publications
The modified log-wake law, which was developed for turbulent boundary layers and pipe flows, is extended to turbulent flows in open-channels. Turbulent velocity profiles in open-channels can be approximated with three components: (1) the law of the wall that results from the constant bed shear stress; (2) the law of the wake that reflects the effects of gravity, secondary currents and bed roughness; and (3) the cubic correction near the maximum velocity. A procedure to determine the four model parameters from velocity measurements while keeping κ = 0.41 is presented. The modified log-wake law compares very well with experimental data …
Implementing Scc Technology In Nebraska—All About Working Together, Mark D. Lafferty
Implementing Scc Technology In Nebraska—All About Working Together, Mark D. Lafferty
Department of Civil and Environmental Engineering: Faculty Publications
In October 2001, Concrete Industries, Inc. (CI) was working with its admixture supplier to improve our form finish for precast concrete products. They brought us a new product and before long the concrete slump became a 26‐in. (660‐mm) diameter circle. Obviously, this generated a lot of excitement with our production personnel, but we also had to consider what it would take to get architects and engineers comfortable with using it.
Chemisorption Of Oxygen Onto Activated Carbon Can Enhance The Stability Of Biological Perchlorate Reduction In Fixed Bed Biofilm Reactors, Young Chul Choi, Xu Li, Lutgarde Raskin, Eberhard Morgenroth
Chemisorption Of Oxygen Onto Activated Carbon Can Enhance The Stability Of Biological Perchlorate Reduction In Fixed Bed Biofilm Reactors, Young Chul Choi, Xu Li, Lutgarde Raskin, Eberhard Morgenroth
Department of Civil and Environmental Engineering: Faculty Publications
Fixed bed biofilm reactors with granular activated carbon (GAC) or glass beads as support media were used to evaluate the influence of short-term (12 h) and long-term (23 days) increases of influent dissolved oxygen (DO) concentrations on biological perchlorate removal. The goal was to evaluate the extent by which chemisorption of oxygen to GAC can enhance the stability of biological perchlorate reduction. Baseline influent concentrations were 50 μg/L of perchlorate, 2 mg/L of acetate as C, and 1 mg/L of DO. Perchlorate removal in the glass bead reactor seized immediately after increasing influent DO concentrations from 1 to 4 mg/L …
Performance Requirements And Evaluation Procedures For Advance Wide Area Detectors, Anuj Sharma, Matthew Harding, Brad Giles, Darcy M. Bullock, James R. Sturdevant, Srinivas Peeta
Performance Requirements And Evaluation Procedures For Advance Wide Area Detectors, Anuj Sharma, Matthew Harding, Brad Giles, Darcy M. Bullock, James R. Sturdevant, Srinivas Peeta
Department of Civil and Environmental Engineering: Faculty Publications
Dilemma zone protection ideally requires the exact position and speed of all the vehicles approaching the intersection. However, point detectors, typically used to protect vehicle incursion into the dilemma zone, only provide binary information about the presence or absence of a vehicle at a fixed point in space. The information regarding position and speed of the vehicle in subsequent time steps is extrapolated. The extrapolation error increases with the increase in projection time. Wide area detector (WAD) overcomes this drawback by tracking each vehicle at all times until they move out of range. Such a detector has the potential to …
Field Evaluation Of Alternative Real-Time Methods For Estimating Approach Delay At Signalized Intersections, Anuj Sharma, Darcy M. Bullock
Field Evaluation Of Alternative Real-Time Methods For Estimating Approach Delay At Signalized Intersections, Anuj Sharma, Darcy M. Bullock
Department of Civil and Environmental Engineering: Faculty Publications
Vehicle delay at a signalized intersection is among the widely used performance measures of efficiency at a signalized intersection. The Highway Capacity Manual (HCM) uses this performance measure to report the level of service at a signalized intersection. This performance measure is also used to estimate travel time along the arterials. With the advent of technological advances in the field of vehicle detection, real-time measurement of delay at a signalized intersection has become economically and computationally feasible. This paper compares three emerging techniques for estimating approach delay: an Input Output technique (IO), a Hybrid technique, and the HCM delay equation. …
Assimilating Remote Sensing-Based Et Into Swap Model For Improved Estimation Of Hydrological Predictions, Baburao Kamble, Ayse Kilic
Assimilating Remote Sensing-Based Et Into Swap Model For Improved Estimation Of Hydrological Predictions, Baburao Kamble, Ayse Kilic
Department of Civil and Environmental Engineering: Faculty Publications
An agro-hydrological simulation model is useful for agriculture monitoring and Remote Sensing provides useful information over large area. Combining both information by data assimilation is used in agro-hydrological modeling and predictions, where multiple remotely sensed data, ground measurement data and model forecast routinely combined in operational mapping procedures. Remote sensing cannot observe input parameters of agro-hydrological models directly. A method to estimate input parameters of such model from Remote Sensing using data assimilation has been proposed by Ines [2002] using the SWAP (Soil, Water, Atmosphere and Plant) model. A Genetic Algorithm (GA) loaded stochastic physically based soil-water-atmosphere-plant model (SWAP) was …