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Nutrient Recycling Potential Of Algal Biomass Grown In Eutrophic Water, Kristin Hampel
Nutrient Recycling Potential Of Algal Biomass Grown In Eutrophic Water, Kristin Hampel
Research and Creative Activities Poster Day
No abstract provided.
Graphene Oxide/Zinc Oxide Nanocomposites As Dissolved Oxygen Sensors, Jameel A. Hasan
Graphene Oxide/Zinc Oxide Nanocomposites As Dissolved Oxygen Sensors, Jameel A. Hasan
Research and Creative Activities Poster Day
No abstract provided.
Multiphoton Fluorescence Spectroscopy To Monitor The Unfolding Of The Green Fluorescent Protein, Ibtesam Y. Alja'afreh, Ramakrishna Guda, David Huffman
Multiphoton Fluorescence Spectroscopy To Monitor The Unfolding Of The Green Fluorescent Protein, Ibtesam Y. Alja'afreh, Ramakrishna Guda, David Huffman
Research and Creative Activities Poster Day
Green fluorescent protein (GFP), from Aequorea coerulescens, has been expressed as a His-tag fusion protein in E. coli cells and purified using metal chelate affinity and gel filtration chromatography. The protein fluoresces at 510 nm due to its natural chromophore that is spontaneously formed after properly folding and remains stable and intact after protein unfolding. The chromophore is buried in a beta barrel and has a permanent dipole moment in both the ground state and the excited state. The difference between permanent dipole moments Δμge is highly proportional to the electrostatic environment around the chromophore. Since Δμge …