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Expression And Purification Of Polyketide Synthase Domains To Investigate Protein-Protein Interactions In Polyketide Biosynthetic Pathways, Mujeeb Adeyinka Wakeel
Expression And Purification Of Polyketide Synthase Domains To Investigate Protein-Protein Interactions In Polyketide Biosynthetic Pathways, Mujeeb Adeyinka Wakeel
Masters Theses
Polyketide synthases (PKSs) are multienzyme proteins catalyzing the production of the polyketide class of natural products with vast structural diversity. Type II PKSs catalyze polyketide biosynthesis using discrete (standalone) enzymes (domains), while type I PKSs use covalently linked enzymes arranged in a module to catalyze polyketide production. It is hypothesized that type II PKSs are the precursors to type I PKSs, proposing that the genes encoding individual discrete enzymes in type II PKSs fused to give the multienzyme modules of type I PKSs. We reasoned that the intramolecular-like reactions occurring in type I PKS modules might be a driving force …