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Protein-Nucleic Acid Interactions In Nuclease And Polymerases, Abdur Rob
Protein-Nucleic Acid Interactions In Nuclease And Polymerases, Abdur Rob
Chemistry Dissertations
DNA polymerase binds to the double stranded DNA and extends the primer strand by adding deoxyribonucletide to the 3’-end. Several reactions in the polymerase active site have been reported by Kornberg in addition to the polymerization. We observed DNA polymerase I can act as a pyrophosphatase and hydrolyze deoxyribonucletide. In performing the pyrophosphatase activity, DNA polymerase I requires to interact with RNA. RNA in general, was found to activate the DNA polymerase I as pyrophosphatase. This hydrolysis causes depletion of dNTP and inhibits DNA polymeration synthesis in vitro. In this RNA-dependent catalysis, DNA polymerase I catalyzes only dNTP but …
Synthesis, Structure And Function Studies Of Selenium And Tellurium Derivatized Nucleic Acids, Jia Sheng
Synthesis, Structure And Function Studies Of Selenium And Tellurium Derivatized Nucleic Acids, Jia Sheng
Chemistry Dissertations
Nucleic acids play important roles in living systems by storing and transferring genetic information and directing protein synthesis. Recently, it was found that nucleic acids can catalyze chemical and biochemical reactions similar to protein enzymes. In addition, they can also serve as drug targets for the treatment of deadly diseases such as AIDS and cancers. As a result, the 3D structure study of nucleic acids and proteinnucleic acids complexes by X-ray crystallography has become one of the most active research areas. However, the two intrinsic bottlenecks of macromolecule X-ray crystallography, including crystallization and phase determination, have significantly limited its application …
Structure And Energetics Of Rna - Protein Interactions For Hiv Rreiib Targeting Zinc Finger Proteins., Subrata H. Mishra
Structure And Energetics Of Rna - Protein Interactions For Hiv Rreiib Targeting Zinc Finger Proteins., Subrata H. Mishra
Chemistry Dissertations
RNA - protein interactions constitute a vital part of numerous biochemical processes. In the HIV life cycle, the interaction of the viral protein Rev and the Rev Responsive Element (RRE), a part of unspliced HIV RNA, is crucial for the propagation of infectious virions. Intervention of this interaction disrupts the viral life cycle. Rev - RRE interaction initially occurs at a high affinity binding site localized to a relatively small stem loop structure called RREIIB. This binding event has been well characterized by a variety of biochemical, enzymatic and structural studies. Our collaborators have previously demonstrated the efficacy of zinc …