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Synthesis, Characterization, And Applications Of Ammonium/Phosphonium Chitosan Derivatives For Investigative Dna Interactions, Quinton Kirk Wyatt

MSU Graduate Theses

Chitosan is examined as a non-viral vector capable of efficient gene delivery due to its favorable properties such as biodegradability, biocompatibility, and is non-toxic to mammalian cells. Cell modifications and vector permeability are enhanced in non-viral vectors that incorporate electrostatic interactions. This work synthesizes cationic (N⁺, P⁺) chitosan derivatives, to enhance electrostatic interactions towards nucleotides, using amide coupling agents EDC and HOBt. The final chitosan derivatives will bear cationic functional groups (N⁺, P⁺) with various alkyl groups (Me, Et, Pr, Ph) to examine their affinities to nucleotides and antifungal properties. mPEG grafted …