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Modification Of Carboplatin By Jurkat Cells, Anthony J. Di Pasqua, Jerry Goodisman, Deborah J. Kerwood, James C. Dabrowiak, Bonnie B. Toms

Chemistry - All Scholarship

Using [¹H,¹⁵N] heteronuclear single quantum coherance (HSQC) NMR and ¹⁵N-labeled carboplatin, 1, we show that Jurkat cells affect the rate of disappearance of the HSQC NMR peak in culture medium for this Pt²⁺ anticancer drug. The decay or disappearance rate constant for 1 in culture medium containing cells is k1=kc[CO32-]+km+kuN, where k_c is the rate constant for reaction of 1 with carbonate in the medium, k_m is the rate constant for reaction of 1 with all other components of the medium, and k_u is the rate constant for reaction of …