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Investigating The Environmental And Molecular Dependence Of Single-Molecule Blinking-Based Multiplexing, Grace Desalvo
Investigating The Environmental And Molecular Dependence Of Single-Molecule Blinking-Based Multiplexing, Grace Desalvo
Dissertations, Theses, and Masters Projects
Visualizing nanoscale biological systems allows us to uncover their detailed structure and functions, which have major implications in the biomedical field. Super-resolution microscopy is a powerful tool for fluorescence imaging because, by overcoming the diffraction limit of light, it accesses structural detail with unprecedented spatial resolution. Although multicolor super-resolution imaging has been successfully implemented in many experiments, its efficiency is limited by reliance on spectral measurement for emitter identification, which limits the combinations of compatible probes to be used together. Blinking-based multiplexing (BBM) is a novel approach that circumvents the need for spectrally-distinct emitters by instead exploiting the intrinsic differences …
Synthesis And Characterization Of Two Sets Of Group 12 Complexes With Organic Ligands, Michelle Sturner
Synthesis And Characterization Of Two Sets Of Group 12 Complexes With Organic Ligands, Michelle Sturner
Dissertations, Theses, and Masters Projects
The complexes of group 12 elements are subjects of incredible interest due to differences in their bioactivity. Zinc is an essential element of life and is required for many bodily processes including gene production, growth, and the immune response. Cadmium and mercury are both toxic elements to humans. Part of their activity is due to their ability to replace zinc metal centers. Synthesis of new group 12 complexes can provide insight regarding their bioactivity and how replacement of metal centers can affect the structure of enzymes. In this thesis, the preparation and characterization of two sets of group 12 complexes …
Gas-Phase Proton Affinities Of Proline- And Pipecolic Acid-Containing Dipeptides, Trinh Ton
Gas-Phase Proton Affinities Of Proline- And Pipecolic Acid-Containing Dipeptides, Trinh Ton
Dissertations, Theses, and Masters Projects
Mass spectrometry (MS) is one of the most used techniques in proteomics because it allows for both high-throughput and quantitative analyses. Bottom-up MS-based proteomics involves breaking down proteins into smaller chains of amino acids called peptides, ionizing and fragmenting the peptides, and identifying the fragments using sequencing databases. These databases depend on the random fragmentation at the backbone peptide bond of the peptides, as predicted by the mobile proton model. Research has shown that peptides containing proline or pipecolic acid have selective fragmentations that could lead to incorrect identification in the sequencing algorithms. These selective cleavages are called “the proline …
Spectroscopy And Dynamics Of Atmospherically And Combustion-Relevant Collision Complexes, John Patrick Davis
Spectroscopy And Dynamics Of Atmospherically And Combustion-Relevant Collision Complexes, John Patrick Davis
Dissertations, Theses, and Masters Projects
Potential energy surfaces describing bimolecular collisions sensitively depend on the chemical functionality and the relative orientation of colliding partners, thus defining the accessibly reactive and nonreactive pathways. Herein, we investigate the peculiar product outcomes arising from Jahn-Teller distortion of the nitric oxide and methane complex (NO-CH4). We have reported an in-depth spectroscopic and dynamics study of NO-CH4 by utilizing conformation-specific and action spectroscopy, as well as velocity map imaging, to understand the fundamental dissociative mechanisms at play. Ultimately, we have gained information about how the Jahn Teller effect possibly impacts the potential product energy transfer pathways. There is a translationally …
Characterizing Molecular Environments In Acrylic Paint Via Single-Sided Nmr, Lyndi Kiple
Characterizing Molecular Environments In Acrylic Paint Via Single-Sided Nmr, Lyndi Kiple
Dissertations, Theses, and Masters Projects
Acrylic paint is a modern artistic material made of colored pigment and polymeric binder. Acrylic binder requires fundamental study at the molecular level to understand its physical properties for purposes of art conservation and general polymer chemistry. The research presented in this thesis uses single-sided nuclear magnetic resonance (NMR) as a non-invasive and non-destructive way to measure relaxation and self-diffusion, which provide insight to molecular mobility and physical properties of proton-containing samples. Specifically, this study relies on T2 relaxation to gain insight to regions within acrylic paint with different molecular mobilities. In both dry and wet paint, relaxometry data revealed …