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Synthesis Of A Fluorescently Tagged Bioactive Probe Specific To Neutrophils In A Zebrafish Model, Sadie Xiaohua Novak
Synthesis Of A Fluorescently Tagged Bioactive Probe Specific To Neutrophils In A Zebrafish Model, Sadie Xiaohua Novak
Honors College
Fluorescent bioimaging has proven to be a powerful tool in non-invasively studying biological processes in living systems. One application of this technique is being used in a transgenic zebrafish model to study the innate immune response to infection of Candida Albicans. Methods utilizing genetic engineering, however, are limited by time constraints that arise when having to depend upon the ability to modify genetics of the model. To bypass these constraints, this project seeks to produce a modified version of a previously developed biofluorescent probe, which was validated in a mouse model. The probe consists of three components, a hexapeptide, …
Interactions Of Gold Thiolates With Protein Disulfides, Anna Tyrina
Interactions Of Gold Thiolates With Protein Disulfides, Anna Tyrina
Honors College
This thesis research focuses on the interaction of gold(I) thiolates with the disulfide bonds in proteins, using insulin as a model protein. Insulin contains three disulfide bonds that can break apart during thiol-disulfide exchange reactions. The goal of this research was to compare the reactivity of aromatic thiols and gold(I) thiolates in thiol-disulfide exchange reactions with insulin. When the disulfide bonds in insulin are cleaved, a suspension of the beta chain particles forms, which scatters 650 nm light, therefore making it convenient to monitor the reaction using UV-Vis spectroscopy. The rate of formation of the colloidal suspension is taken to …