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Fabrication, Characterization, And Application Of Carbon Nanoparticles For The Detection Of Heavy Metal Ions In Aqueous Media, Aaron Michael Simpson
Fabrication, Characterization, And Application Of Carbon Nanoparticles For The Detection Of Heavy Metal Ions In Aqueous Media, Aaron Michael Simpson
MSU Graduate Theses
This research set out to develop a method for producing Carbon nanoparticles (CNPs) from glycerol and use them to detect Cu2+, Hg2+, and Pb2+ ions in aqueous solution. Three synthesis methods were developed using the following mixtures: (1) glycerol and silica, (2) glycerol, silica, and H3PO4, and (3) glycerol with H3PO4. The structure of the particles was characterized using a variety of spectroscopic techniques. Size distributions were obtained from scanning electron microscopy images. The particles were spherical with average diameters of 66, 58, and 89 nm respectively and believed to consist of a carbon core with carboxylic acid and alcohol …
Synthesis And Functionalization Of Fluorescent Quantum Dot Bioconjugates For Cellular Imaging Of Directed Gene Therapy, Jason Matthew Davis
Synthesis And Functionalization Of Fluorescent Quantum Dot Bioconjugates For Cellular Imaging Of Directed Gene Therapy, Jason Matthew Davis
MSU Graduate Theses
Herein, I optimize a method for synthesis and bioconjugation of water-soluble, fluorescent CdSe/ZnS quantum dots (QDs) for targeted cellular delivery of DNA. Core CdSe QDs were synthesized in high temperature organic solvents and passivated with a ZnS shell to increase quantum yield. The fluorescent QD nanoparticles were made water-soluble by enveloping them with an amphiphilic polymer. These aqueous nanoparticles were functionalized with tertiary amines to impart a positive charge, allowing electrostatic binding to negatively-charged DNA. The conjugated QDs were characterized using zeta potential and electrophoresis to gauge their ability to electrostatically bind DNA. The QDs were further modified by conjugation …