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Simultaneous Determination Of Glucose And L‐Glutamate Using A Capillary Enzyme Reactor With Electrochemical Detection, Stephanie Hooper, Mark Anderson
Simultaneous Determination Of Glucose And L‐Glutamate Using A Capillary Enzyme Reactor With Electrochemical Detection, Stephanie Hooper, Mark Anderson
Mark R. Anderson
An electrophoresis capillary that incorporates two enzymes for the simultaneous determination of glucose and l-glutamate is described. The enzymes deposited along the separation capillary walls react with their respective substrate as they are separated during the electrophoresis to produce hydrogen peroxide that is detected by amperometry as the hydrogen peroxide zone emerges from the end of the capillary. Even though both enzyme reactions produce a common product, hydrogen peroxide, the hydrogen peroxide produced by each enzyme reaction stays in narrow zones that migrate the length of the capillary at different rates. The rate of migration for the individual H2O2 zones …