Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Entire DC Network
The Effects Of Mismatches And Probe Tethering Configurations On The Stability Of Dna Duplexes On Surfaces, Kyle Evan Pratt
The Effects Of Mismatches And Probe Tethering Configurations On The Stability Of Dna Duplexes On Surfaces, Kyle Evan Pratt
Theses and Dissertations
DNA microarrays are chip-based, analysis tools which can perform hundreds of thousands of parallel assays to determine the identity of genes or gene expression levels present in a sample. They have been identified as a key technology in genomic sciences and emergent medical techniques; however, despite their abundant use in research laboratories, microarrays have not been used in the clinical setting to the fullest potential due to the difficulty of obtaining reproducible results. Microarrays work on the principle of DNA hybridization, and can only be as accurate as this process is robust. Fundamental, molecular-level understanding of hybridization on surfaces is …
Protein-Surface Interactions With Coarse-Grain Simulation Methods, Shuai Wei
Protein-Surface Interactions With Coarse-Grain Simulation Methods, Shuai Wei
Theses and Dissertations
The interaction of proteins with surfaces is a major process involved in protein microarrays. Understanding protein-surface interactions is key to improving the performance of protein microarrays, but current understanding of the behavior of proteins on surfaces is lacking. Prevailing theories on the subject, which suggest that proteins should be stabilized when tethered to surfaces, do not explain the experimentally observed fact that proteins are often denatured on surfaces. This document outlines several studies done to develop a model which is capable of predicting the stabilization and destabilization of proteins tethered to surfaces. As the start point of the research, part …