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Quantitative Elucidation Of A Distinct Spatial Gradient-Sensing Mechanism In Fibroblasts, Ian C. Schneider, Jason M. Haugh
Quantitative Elucidation Of A Distinct Spatial Gradient-Sensing Mechanism In Fibroblasts, Ian C. Schneider, Jason M. Haugh
Ian C. Schneider
Migration of eukaryotic cells toward a chemoattractant often relies on their ability to distinguish receptor-mediated signaling at different subcellular locations, a phenomenon known as spatial sensing. A prominent example that is seen during wound healing is fibroblast migration in platelet-derived growth factor (PDGF) gradients. As in the well-characterized chemotactic cells Dictyostelium discoideum and neutrophils, signaling to the cytoskeleton via the phosphoinositide 3-kinase pathway in fibroblasts is spatially polarized by a PDGF gradient; however, the sensitivity of this process and how it is regulated are unknown. Through a quantitative analysis of mathematical models and live cell total internal reflection fluorescence microscopy …