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Effect Of Differences In The Primary Structure Of The A-Chain On The Aggregation Of Insulin Fragments, Paul P. Nakka, Ke Li, Daniel Forciniti
Effect Of Differences In The Primary Structure Of The A-Chain On The Aggregation Of Insulin Fragments, Paul P. Nakka, Ke Li, Daniel Forciniti
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Bovine and human insulin have similar primary structures. In this article, the region of the insulin A-chain of bovine and human insulin where the amino acid composition is different was studied. Bovine insulin fragment (BIF) and human insulin fragment (HIF) were synthesized in solid-phase peptide synthesis. The effects of pH, temperature, urea, ionic strength, and stirring on the formation of fibrils were studied using a fractional factorial resolution III experimental design. Fibrillation was monitored by fluorescence and infrared spectroscopy and optical microscopy. Both fragments formed fibrils at pH 1.6 and a temperature of 60 °C. The lag time and apparent …