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Investigations Into Avian Skeletal Adaptations And Spine Fusion Events, Tori Huey Aug 2022

Investigations Into Avian Skeletal Adaptations And Spine Fusion Events, Tori Huey

All Theses

Avian dinosaurs evolved skeletal adaptations resulting in numerous fused spinal structures, like the synsacrum, present in extant birds. The synsacrum is a highly fused region necessary for stiffening the spine, and it fuses the spine to the pelvic ilium. Conventional wisdom suggests that fusion occurred in ovo during embryonic development via the cartilage matrix that patterns the future vertebrae. We show that post hatch birds have intervertebral discs separating all vertebrae of the spine, leading us to re-examine this notion in chickens up to 10 weeks of age. Intervertebral discs undergo constant remodeling, then degenerate and undergo tissue clearance, followed …


Unraveling The Genetic Architecture Of Somatic Embryogenesis In Upland Cotton, Adam M. Canal May 2022

Unraveling The Genetic Architecture Of Somatic Embryogenesis In Upland Cotton, Adam M. Canal

All Theses

Somatic embryogenesis is the de novo development of asexual embryos because of the plasticity of the plant cell. In tissue culture, the biochemical and genetic mechanisms of dedifferentiated callus tissues can be reprogrammed to transdifferentiate into developed, polarized embryos, which can ultimately regenerate into whole plants. Although this rarely occurs in nature, scientists have exploited this process for decades to regenerate whole plants following gene transformation or for micropropagation. While some species are amenable to in vitro regeneration, upland cotton is particularly recalcitrant, with regenerative potential being confined to only several genotypes. The lack of elite, regenerable genotypes greatly restricts …


Effects Of 2,4-Di-Tert-Butylphenol On Osteogenic And Myogenic Differentiation Of Human Induced Pluripotent Stem Cells, Thanh-Bình Dương May 2022

Effects Of 2,4-Di-Tert-Butylphenol On Osteogenic And Myogenic Differentiation Of Human Induced Pluripotent Stem Cells, Thanh-Bình Dương

All Theses

Synthetic phenolic antioxidants (SPAs) are a class of compounds used to increase the durability of polymers as well as to increase the shelf-life of food, cosmetics, and other personal care items. Of these SPAs is 2,4-di-tert-butylphenol (2,4-DBP). 2,4-DBP is primarily used in polyethylene crosspolymer pipes, which are now used as a replacement for the traditional copper pipes for household water distribution. However, 2,4-DBP has been found to leach from these pipes into the water, enabling a mode of exposure to humans. 2,4-DBP has been detected in human urine, serum, but more importantly, maternal serum, placenta, and cord blood. …